Entometabolomics: applications of modern analytical techniques to insect studies
Metabolomic analyses can reveal associations between an organism's metabolome and further aspects of its phenotypic state, an attractive prospect for many life-sciences researchers. The metabolomic approach has been employed in some, but not many, insect study systems, starting in 1990 with the...
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| Format: | Article |
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Wiley
2015
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| Online Access: | https://eprints.nottingham.ac.uk/41500/ |
| _version_ | 1848796288187891712 |
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| author | Snart, Charles J.P. Hardy, Ian C.W. Barrett, David A. |
| author_facet | Snart, Charles J.P. Hardy, Ian C.W. Barrett, David A. |
| author_sort | Snart, Charles J.P. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Metabolomic analyses can reveal associations between an organism's metabolome and further aspects of its phenotypic state, an attractive prospect for many life-sciences researchers. The metabolomic approach has been employed in some, but not many, insect study systems, starting in 1990 with the evaluation of the metabolic effects of parasitism on moth larvae. Metabolomics has now been applied to a variety of aspects of insect biology, including behaviour, infection, temperature stress responses, CO2 sedation, and bacteria–insect symbiosis. From a technical and reporting standpoint, these studies have adopted a range of approaches utilising established experimental methodologies. Here, we review current literature and evaluate the metabolomic approaches typically utilised by entomologists. We suggest that improvements can be made in several areas, including sampling procedures, the reduction in sampling and equipment variation, the use of sample extracts, statistical analyses, confirmation, and metabolite identification. Overall, it is clear that metabolomics can identify correlations between phenotypic states and underlying cellular metabolism that previous, more targeted, approaches are incapable of measuring. The unique combination of untargeted global analyses with high-resolution quantitative analyses results in a tool with great potential for future entomological investigations. |
| first_indexed | 2025-11-14T19:45:36Z |
| format | Article |
| id | nottingham-41500 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:45:36Z |
| publishDate | 2015 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-415002020-05-04T20:09:21Z https://eprints.nottingham.ac.uk/41500/ Entometabolomics: applications of modern analytical techniques to insect studies Snart, Charles J.P. Hardy, Ian C.W. Barrett, David A. Metabolomic analyses can reveal associations between an organism's metabolome and further aspects of its phenotypic state, an attractive prospect for many life-sciences researchers. The metabolomic approach has been employed in some, but not many, insect study systems, starting in 1990 with the evaluation of the metabolic effects of parasitism on moth larvae. Metabolomics has now been applied to a variety of aspects of insect biology, including behaviour, infection, temperature stress responses, CO2 sedation, and bacteria–insect symbiosis. From a technical and reporting standpoint, these studies have adopted a range of approaches utilising established experimental methodologies. Here, we review current literature and evaluate the metabolomic approaches typically utilised by entomologists. We suggest that improvements can be made in several areas, including sampling procedures, the reduction in sampling and equipment variation, the use of sample extracts, statistical analyses, confirmation, and metabolite identification. Overall, it is clear that metabolomics can identify correlations between phenotypic states and underlying cellular metabolism that previous, more targeted, approaches are incapable of measuring. The unique combination of untargeted global analyses with high-resolution quantitative analyses results in a tool with great potential for future entomological investigations. Wiley 2015-04 Article PeerReviewed Snart, Charles J.P., Hardy, Ian C.W. and Barrett, David A. (2015) Entometabolomics: applications of modern analytical techniques to insect studies. Entomologia Experimentalis et Applicata, 155 (1). pp. 1-17. ISSN 1570-7458 nuclear magnetic resonance gas chromatography-mass spectrometry liquid chromatography-mass spectrometry multivariate data analysis http://onlinelibrary.wiley.com/doi/10.1111/eea.12281/abstract doi:10.1111/eea.12281 doi:10.1111/eea.12281 |
| spellingShingle | nuclear magnetic resonance gas chromatography-mass spectrometry liquid chromatography-mass spectrometry multivariate data analysis Snart, Charles J.P. Hardy, Ian C.W. Barrett, David A. Entometabolomics: applications of modern analytical techniques to insect studies |
| title | Entometabolomics: applications of modern analytical techniques to insect studies |
| title_full | Entometabolomics: applications of modern analytical techniques to insect studies |
| title_fullStr | Entometabolomics: applications of modern analytical techniques to insect studies |
| title_full_unstemmed | Entometabolomics: applications of modern analytical techniques to insect studies |
| title_short | Entometabolomics: applications of modern analytical techniques to insect studies |
| title_sort | entometabolomics: applications of modern analytical techniques to insect studies |
| topic | nuclear magnetic resonance gas chromatography-mass spectrometry liquid chromatography-mass spectrometry multivariate data analysis |
| url | https://eprints.nottingham.ac.uk/41500/ https://eprints.nottingham.ac.uk/41500/ https://eprints.nottingham.ac.uk/41500/ |