Genetic diversity of porcine group A rotavirus strains in the UK

Rotavirus is endemic in pig farms where it causes a loss in production. This study is the first to characterise porcine rotavirus circulating in UK pigs. Samples from diarrheic pigs with rotavirus enteritis obtained between 2010 and 2012 were genotyped in order to determine the diversity of group A...

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Main Authors: Chandler-Bostock, Rebecca, Hancox, Laura R., Nawaz, Sameena, Watts, Oliver, Iturriza-Gomara, Miren, Mellits, Kenneth M.
Format: Article
Published: Elsevier 2014
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Online Access:https://eprints.nottingham.ac.uk/41476/
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author Chandler-Bostock, Rebecca
Hancox, Laura R.
Nawaz, Sameena
Watts, Oliver
Iturriza-Gomara, Miren
Mellits, Kenneth M.
author_facet Chandler-Bostock, Rebecca
Hancox, Laura R.
Nawaz, Sameena
Watts, Oliver
Iturriza-Gomara, Miren
Mellits, Kenneth M.
author_sort Chandler-Bostock, Rebecca
building Nottingham Research Data Repository
collection Online Access
description Rotavirus is endemic in pig farms where it causes a loss in production. This study is the first to characterise porcine rotavirus circulating in UK pigs. Samples from diarrheic pigs with rotavirus enteritis obtained between 2010 and 2012 were genotyped in order to determine the diversity of group A rotavirus (GARV) in UK pigs. A wide range of rotavirus genotypes were identified in UK pigs: six G types (VP7); G2, G3, G4, G5, G9 and G11 and six P types (VP4); P[6], P[7], P[8], P[13], P[23], and P[32]. With the exception of a single P[8] isolate, there was less than 95% nucleotide identity between sequences from this study and any available rotavirus sequences. The G9 and P[6] genotypes are capable of infecting both humans and pigs, but showed no species cross-over within the UK as they were shown to be genetically distinct, which suggested zoonotic transmission is rare within the UK. We identified the P[8] genotype in one isolate, this genotype is almost exclusively found in humans. The P[8] was linked to a human Irish rotavirus isolate in the same year. The discovery of human genotype P[8] rotavirus in a UK pig confirms this common human genotype can infect pigs and also highlights the necessity of surveillance of porcine rotavirus genotypes to safeguard human as well as porcine health.
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spelling nottingham-414762020-05-04T16:53:52Z https://eprints.nottingham.ac.uk/41476/ Genetic diversity of porcine group A rotavirus strains in the UK Chandler-Bostock, Rebecca Hancox, Laura R. Nawaz, Sameena Watts, Oliver Iturriza-Gomara, Miren Mellits, Kenneth M. Rotavirus is endemic in pig farms where it causes a loss in production. This study is the first to characterise porcine rotavirus circulating in UK pigs. Samples from diarrheic pigs with rotavirus enteritis obtained between 2010 and 2012 were genotyped in order to determine the diversity of group A rotavirus (GARV) in UK pigs. A wide range of rotavirus genotypes were identified in UK pigs: six G types (VP7); G2, G3, G4, G5, G9 and G11 and six P types (VP4); P[6], P[7], P[8], P[13], P[23], and P[32]. With the exception of a single P[8] isolate, there was less than 95% nucleotide identity between sequences from this study and any available rotavirus sequences. The G9 and P[6] genotypes are capable of infecting both humans and pigs, but showed no species cross-over within the UK as they were shown to be genetically distinct, which suggested zoonotic transmission is rare within the UK. We identified the P[8] genotype in one isolate, this genotype is almost exclusively found in humans. The P[8] was linked to a human Irish rotavirus isolate in the same year. The discovery of human genotype P[8] rotavirus in a UK pig confirms this common human genotype can infect pigs and also highlights the necessity of surveillance of porcine rotavirus genotypes to safeguard human as well as porcine health. Elsevier 2014-09-17 Article PeerReviewed Chandler-Bostock, Rebecca, Hancox, Laura R., Nawaz, Sameena, Watts, Oliver, Iturriza-Gomara, Miren and Mellits, Kenneth M. (2014) Genetic diversity of porcine group A rotavirus strains in the UK. Veterinary Microbiology, 173 (1-2). pp. 27-37. ISSN 1873-2542 Rotavirus; Porcine; Phylogenetic; Zoonosis http://www.sciencedirect.com/science/article/pii/S0378113514003228 doi:10.1016/j.vetmic.2014.06.030 doi:10.1016/j.vetmic.2014.06.030
spellingShingle Rotavirus; Porcine; Phylogenetic; Zoonosis
Chandler-Bostock, Rebecca
Hancox, Laura R.
Nawaz, Sameena
Watts, Oliver
Iturriza-Gomara, Miren
Mellits, Kenneth M.
Genetic diversity of porcine group A rotavirus strains in the UK
title Genetic diversity of porcine group A rotavirus strains in the UK
title_full Genetic diversity of porcine group A rotavirus strains in the UK
title_fullStr Genetic diversity of porcine group A rotavirus strains in the UK
title_full_unstemmed Genetic diversity of porcine group A rotavirus strains in the UK
title_short Genetic diversity of porcine group A rotavirus strains in the UK
title_sort genetic diversity of porcine group a rotavirus strains in the uk
topic Rotavirus; Porcine; Phylogenetic; Zoonosis
url https://eprints.nottingham.ac.uk/41476/
https://eprints.nottingham.ac.uk/41476/
https://eprints.nottingham.ac.uk/41476/