Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls
Objective: To elucidate the natural course of circulating insulin-like peptide 3 (INSL3) levels according to puberty as well as its relation to other reproductive hormones. Design: Population-based cohort study. Setting: Not applicable. Patient(s): Healthy peripubertal girls (n¼10) examined every...
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| Format: | Article |
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Elsevier
2015
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| Online Access: | https://eprints.nottingham.ac.uk/41445/ |
| _version_ | 1848796275222249472 |
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| author | Hagen, Casper P. Mieritz, Mikkel G. Nielsen, John E. Anand-Ivell, Ravinder Ivell, Richard Juul, Anders |
| author_facet | Hagen, Casper P. Mieritz, Mikkel G. Nielsen, John E. Anand-Ivell, Ravinder Ivell, Richard Juul, Anders |
| author_sort | Hagen, Casper P. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Objective: To elucidate the natural course of circulating insulin-like peptide 3 (INSL3) levels according to puberty as well as its relation to other reproductive hormones.
Design: Population-based cohort study.
Setting: Not applicable.
Patient(s): Healthy peripubertal girls (n¼10) examined every 6 months; total number of examinations was 84; median (range) per girl:9 (4–10), including staging of pubertal breast development and blood samples.
Intervention(s): None.
Main Outcome Measure(s): Serum levels of INSL3, inhibin B, E2, antim€ullerian hormone, LH, and FSH (validated immunoassays), and T and androstenedione (liquid chromatography–tandem mass spectrometry).
Result(s): Serum levels of INSL3 varied considerably between girls (range, 0.01–0.27 ng/mL) and within each girl as puberty progressed; intraindividual variation, median (range) 102% (65%–143%). Insulin-like peptide 3 increased in late puberty (B1 to B4þB5); geometric mean 0.03 ng/mL to 0.15 ng/mL. Insulin-like peptide 3 levels reflected markers of large follicles (T, androstendione, inhibin B, and E2) better than markers of small follicles (antim€ullerian hormone), and INSL3 staining was localized in theca interna cells of antral follicles.
Conclusion(s): Insulin-like peptide 3 increased in late puberty, albeit inter- and intraindividual variations were substantial. Immunohistochemistry and intraindividual variation, as well as relations to other ovarian hormones, reveal that INSL3 in girls is a unique and specific marker of theca cells surrounding antral follicles. The potential clinical use of INSL3 for evaluation of ovarian function in girls remains to be elucidated. |
| first_indexed | 2025-11-14T19:45:23Z |
| format | Article |
| id | nottingham-41445 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:45:23Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-414452020-05-04T17:01:38Z https://eprints.nottingham.ac.uk/41445/ Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls Hagen, Casper P. Mieritz, Mikkel G. Nielsen, John E. Anand-Ivell, Ravinder Ivell, Richard Juul, Anders Objective: To elucidate the natural course of circulating insulin-like peptide 3 (INSL3) levels according to puberty as well as its relation to other reproductive hormones. Design: Population-based cohort study. Setting: Not applicable. Patient(s): Healthy peripubertal girls (n¼10) examined every 6 months; total number of examinations was 84; median (range) per girl:9 (4–10), including staging of pubertal breast development and blood samples. Intervention(s): None. Main Outcome Measure(s): Serum levels of INSL3, inhibin B, E2, antim€ullerian hormone, LH, and FSH (validated immunoassays), and T and androstenedione (liquid chromatography–tandem mass spectrometry). Result(s): Serum levels of INSL3 varied considerably between girls (range, 0.01–0.27 ng/mL) and within each girl as puberty progressed; intraindividual variation, median (range) 102% (65%–143%). Insulin-like peptide 3 increased in late puberty (B1 to B4þB5); geometric mean 0.03 ng/mL to 0.15 ng/mL. Insulin-like peptide 3 levels reflected markers of large follicles (T, androstendione, inhibin B, and E2) better than markers of small follicles (antim€ullerian hormone), and INSL3 staining was localized in theca interna cells of antral follicles. Conclusion(s): Insulin-like peptide 3 increased in late puberty, albeit inter- and intraindividual variations were substantial. Immunohistochemistry and intraindividual variation, as well as relations to other ovarian hormones, reveal that INSL3 in girls is a unique and specific marker of theca cells surrounding antral follicles. The potential clinical use of INSL3 for evaluation of ovarian function in girls remains to be elucidated. Elsevier 2015-03-01 Article PeerReviewed Hagen, Casper P., Mieritz, Mikkel G., Nielsen, John E., Anand-Ivell, Ravinder, Ivell, Richard and Juul, Anders (2015) Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls. Fertility and Sterility, 103 (3). pp. 780-786. ISSN 0015-0282 INSL3 puberty theca girl follicle http://www.sciencedirect.com/science/article/pii/S0015028214023607 doi:10.1016/j.fertnstert.2014.11.014 doi:10.1016/j.fertnstert.2014.11.014 |
| spellingShingle | INSL3 puberty theca girl follicle Hagen, Casper P. Mieritz, Mikkel G. Nielsen, John E. Anand-Ivell, Ravinder Ivell, Richard Juul, Anders Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls |
| title | Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls |
| title_full | Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls |
| title_fullStr | Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls |
| title_full_unstemmed | Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls |
| title_short | Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls |
| title_sort | longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls |
| topic | INSL3 puberty theca girl follicle |
| url | https://eprints.nottingham.ac.uk/41445/ https://eprints.nottingham.ac.uk/41445/ https://eprints.nottingham.ac.uk/41445/ |