Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them
For several decades antibodies raised against specific proteins, peptides, or peptide epitopes have proven to be versatile and very powerful tools to demonstrate molecular identity in cells and tissues. New techniques of immunohistochemistry and immunofluorescence have improved both the optical reso...
| Main Authors: | , , |
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| Format: | Article |
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Endocrine Society
2014
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| Online Access: | https://eprints.nottingham.ac.uk/41434/ |
| _version_ | 1848796271774531584 |
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| author | Ivell, Richard Teerds, Katja Hoffman, Gloria E |
| author_facet | Ivell, Richard Teerds, Katja Hoffman, Gloria E |
| author_sort | Ivell, Richard |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | For several decades antibodies raised against specific proteins, peptides, or peptide epitopes have proven to be versatile and very powerful tools to demonstrate molecular identity in cells and tissues. New techniques of immunohistochemistry and immunofluorescence have improved both the optical resolution of such protein identification as well as its sensitivity, particularly through the use of amplification methodology. However, this improved sensitivity has also increased the risks of false-positive and false-negative staining and thereby raised the necessity for proper and adequate controls. In this review, the authors drawonmanyyears of experience to illuminate many of the more common errors and problematic issues in immunohistochemistry, and how these may be avoided. A key factor in all of this is that techniques need to be properly documented and especially antibodies and procedures must be adequately described. Antibodies are a valuable and shared resource within the scientific community; it is essential therefore that mistakes involving antibodies and their controls are not perpetuated through inadequate reporting in the literature. |
| first_indexed | 2025-11-14T19:45:20Z |
| format | Article |
| id | nottingham-41434 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:45:20Z |
| publishDate | 2014 |
| publisher | Endocrine Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-414342020-05-04T16:42:29Z https://eprints.nottingham.ac.uk/41434/ Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them Ivell, Richard Teerds, Katja Hoffman, Gloria E For several decades antibodies raised against specific proteins, peptides, or peptide epitopes have proven to be versatile and very powerful tools to demonstrate molecular identity in cells and tissues. New techniques of immunohistochemistry and immunofluorescence have improved both the optical resolution of such protein identification as well as its sensitivity, particularly through the use of amplification methodology. However, this improved sensitivity has also increased the risks of false-positive and false-negative staining and thereby raised the necessity for proper and adequate controls. In this review, the authors drawonmanyyears of experience to illuminate many of the more common errors and problematic issues in immunohistochemistry, and how these may be avoided. A key factor in all of this is that techniques need to be properly documented and especially antibodies and procedures must be adequately described. Antibodies are a valuable and shared resource within the scientific community; it is essential therefore that mistakes involving antibodies and their controls are not perpetuated through inadequate reporting in the literature. Endocrine Society 2014-03-01 Article PeerReviewed Ivell, Richard, Teerds, Katja and Hoffman, Gloria E (2014) Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them. Endocrinology, 155 (3). pp. 676-687. ISSN 1945-7170 https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2013-1971 doi:10.1210/en.2013-1971 doi:10.1210/en.2013-1971 |
| spellingShingle | Ivell, Richard Teerds, Katja Hoffman, Gloria E Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them |
| title | Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them |
| title_full | Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them |
| title_fullStr | Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them |
| title_full_unstemmed | Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them |
| title_short | Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them |
| title_sort | proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them |
| url | https://eprints.nottingham.ac.uk/41434/ https://eprints.nottingham.ac.uk/41434/ https://eprints.nottingham.ac.uk/41434/ |