Perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal Leydig cell function, and cryptorchidism and hypospadias in Danish boys (1980–1996)

Background: Exposure to perfluorooctane sulfonate (PFOS) may potentially disturb fetal Leydig cell hormone production and male genital development. Objectives: We aimed to study the associations between levels of amniotic fluid PFOS, fetal steroid hormone, and insulin-like factor 3 (INSL3) and the...

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Main Authors: Toft, Gunnar, Jönsson, Bo A.G., Bonde, Jens Peter, Nørgaard-Pedersen, Bent, Hougaard, David M., Cohen, Arieh, Lindh, Christian H., Ivell, Richard, Anand-Ivell, Ravinder, Lindhard, Morten S.
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Published: National Institute of Environmental Health Sciences 2016
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Online Access:https://eprints.nottingham.ac.uk/41433/
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author Toft, Gunnar
Jönsson, Bo A.G.
Bonde, Jens Peter
Nørgaard-Pedersen, Bent
Hougaard, David M.
Cohen, Arieh
Lindh, Christian H.
Ivell, Richard
Anand-Ivell, Ravinder
Lindhard, Morten S.
author_facet Toft, Gunnar
Jönsson, Bo A.G.
Bonde, Jens Peter
Nørgaard-Pedersen, Bent
Hougaard, David M.
Cohen, Arieh
Lindh, Christian H.
Ivell, Richard
Anand-Ivell, Ravinder
Lindhard, Morten S.
author_sort Toft, Gunnar
building Nottingham Research Data Repository
collection Online Access
description Background: Exposure to perfluorooctane sulfonate (PFOS) may potentially disturb fetal Leydig cell hormone production and male genital development. Objectives: We aimed to study the associations between levels of amniotic fluid PFOS, fetal steroid hormone, and insulin-like factor 3 (INSL3) and the prevalence of cryptorchidism and hypospadias. Methods: Using the Danish National Patient Registry, we selected 270 cryptorchidism cases,75 hypospadias cases, and 300 controls with stored maternal amniotic fluid samples available in a Danish pregnancy-screening biobank (1980–1996). We used mass spectrometry to measure PFOS in amniotic fluid from 645 persons and steroid hormones in samples from 545 persons. INSL3 was measured by immunoassay from 475 persons. Associations between PFOS concentration in amniotic fluid, hormone levels, and genital malformations were assessed by confounder-adjusted linear and logistic regression. Results: The highest tertile of PFOS exposure (> 1.4 ng/mL) in amniotic fluid was associated with a 40% (95% CI: –69, –11%) lower INSL3 level and an 18% (95% CI: 7, 29%) higher testosterone level compared with the lowest tertile (< 0.8 ng/mL). Amniotic fluid PFOS concentration was not associated with cryptorchidism or hypospadias. Conclusions: Environmental PFOS exposure was associated with steroid hormone and INSL3 concentrations in amniotic fluid, but was not associated with cryptorchidism or hypospadias in our study population. Additional studies are needed to determine whether associations with fetal hormone levels may have long-term implications for reproductive health.
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spelling nottingham-414332020-05-04T17:25:01Z https://eprints.nottingham.ac.uk/41433/ Perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal Leydig cell function, and cryptorchidism and hypospadias in Danish boys (1980–1996) Toft, Gunnar Jönsson, Bo A.G. Bonde, Jens Peter Nørgaard-Pedersen, Bent Hougaard, David M. Cohen, Arieh Lindh, Christian H. Ivell, Richard Anand-Ivell, Ravinder Lindhard, Morten S. Background: Exposure to perfluorooctane sulfonate (PFOS) may potentially disturb fetal Leydig cell hormone production and male genital development. Objectives: We aimed to study the associations between levels of amniotic fluid PFOS, fetal steroid hormone, and insulin-like factor 3 (INSL3) and the prevalence of cryptorchidism and hypospadias. Methods: Using the Danish National Patient Registry, we selected 270 cryptorchidism cases,75 hypospadias cases, and 300 controls with stored maternal amniotic fluid samples available in a Danish pregnancy-screening biobank (1980–1996). We used mass spectrometry to measure PFOS in amniotic fluid from 645 persons and steroid hormones in samples from 545 persons. INSL3 was measured by immunoassay from 475 persons. Associations between PFOS concentration in amniotic fluid, hormone levels, and genital malformations were assessed by confounder-adjusted linear and logistic regression. Results: The highest tertile of PFOS exposure (> 1.4 ng/mL) in amniotic fluid was associated with a 40% (95% CI: –69, –11%) lower INSL3 level and an 18% (95% CI: 7, 29%) higher testosterone level compared with the lowest tertile (< 0.8 ng/mL). Amniotic fluid PFOS concentration was not associated with cryptorchidism or hypospadias. Conclusions: Environmental PFOS exposure was associated with steroid hormone and INSL3 concentrations in amniotic fluid, but was not associated with cryptorchidism or hypospadias in our study population. Additional studies are needed to determine whether associations with fetal hormone levels may have long-term implications for reproductive health. National Institute of Environmental Health Sciences 2016-01-01 Article PeerReviewed Toft, Gunnar, Jönsson, Bo A.G., Bonde, Jens Peter, Nørgaard-Pedersen, Bent, Hougaard, David M., Cohen, Arieh, Lindh, Christian H., Ivell, Richard, Anand-Ivell, Ravinder and Lindhard, Morten S. (2016) Perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal Leydig cell function, and cryptorchidism and hypospadias in Danish boys (1980–1996). Environmental Health Perspectives, 124 (1). pp. 151-156. ISSN 0091-6765 Amniotic fluid endocrine disruptors phthalates https://ehp.niehs.nih.gov/1409288/ doi:10.1289/ehp.1409288 doi:10.1289/ehp.1409288
spellingShingle Amniotic fluid
endocrine disruptors
phthalates
Toft, Gunnar
Jönsson, Bo A.G.
Bonde, Jens Peter
Nørgaard-Pedersen, Bent
Hougaard, David M.
Cohen, Arieh
Lindh, Christian H.
Ivell, Richard
Anand-Ivell, Ravinder
Lindhard, Morten S.
Perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal Leydig cell function, and cryptorchidism and hypospadias in Danish boys (1980–1996)
title Perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal Leydig cell function, and cryptorchidism and hypospadias in Danish boys (1980–1996)
title_full Perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal Leydig cell function, and cryptorchidism and hypospadias in Danish boys (1980–1996)
title_fullStr Perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal Leydig cell function, and cryptorchidism and hypospadias in Danish boys (1980–1996)
title_full_unstemmed Perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal Leydig cell function, and cryptorchidism and hypospadias in Danish boys (1980–1996)
title_short Perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal Leydig cell function, and cryptorchidism and hypospadias in Danish boys (1980–1996)
title_sort perfluorooctane sulfonate concentrations in amniotic fluid, biomarkers of fetal leydig cell function, and cryptorchidism and hypospadias in danish boys (1980–1996)
topic Amniotic fluid
endocrine disruptors
phthalates
url https://eprints.nottingham.ac.uk/41433/
https://eprints.nottingham.ac.uk/41433/
https://eprints.nottingham.ac.uk/41433/