Mouse mitochondrial lipid composition is defined by age in brain and muscle
Functionality of the lipid rich mitochondrial organelle declines with increased age. Recent advances in lipidomic technologies allowed us to perform a global characterisation of lipid composition in two different tissue types and age ranges. Ultra-high performance liquid chromatography coupled with...
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Impact Journals
2017
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| Online Access: | https://eprints.nottingham.ac.uk/41327/ |
| _version_ | 1848796249528991744 |
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| author | Pollard, Amelia K. Ortori, Catharine A. Stöger, Reinhard Barrett, David A. Chakrabarti, Lisa |
| author_facet | Pollard, Amelia K. Ortori, Catharine A. Stöger, Reinhard Barrett, David A. Chakrabarti, Lisa |
| author_sort | Pollard, Amelia K. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Functionality of the lipid rich mitochondrial organelle declines with increased age. Recent advances in lipidomic technologies allowed us to perform a global characterisation of lipid composition in two different tissue types and age ranges. Ultra-high performance liquid chromatography coupled with high resolution mass spectrometry was used to establish and compare mitochondrial lipidomes of brain and skeletal muscle from young (4-11 weeks old) and middle age (78 weeks old) healthy mice. In middle age the brain mitochondria had reduced levels of fatty acids, particularly polyunsaturated fatty acids, while skeletal muscle mitochondria had a decreased abundance of phosphatidylethanolamine, but a pronounced increase of triglyceride levels. Reduced levels of phosphatidylethanolamines are known to decrease mitochondrial membrane fluidity and are connected with accelerated ageing. In mitochondria from skeletal muscle we propose that increased age causes a metabolic shift in the conversion of diacylglycerol so that triglycerides predominate compared with phosphatidylethanolamines. This is the first time mitochondrial lipid content in normal healthy mammalian ageing brain and muscle has been catalogued in detail across all lipid classes. We identify distinct mitochondrial lipid signatures that change with age, revealing tissue-specific lipid pathways as possible targets to ameliorate ageing-related mitochondrial decline. |
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| format | Article |
| id | nottingham-41327 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:44:59Z |
| publishDate | 2017 |
| publisher | Impact Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-413272020-05-04T18:38:23Z https://eprints.nottingham.ac.uk/41327/ Mouse mitochondrial lipid composition is defined by age in brain and muscle Pollard, Amelia K. Ortori, Catharine A. Stöger, Reinhard Barrett, David A. Chakrabarti, Lisa Functionality of the lipid rich mitochondrial organelle declines with increased age. Recent advances in lipidomic technologies allowed us to perform a global characterisation of lipid composition in two different tissue types and age ranges. Ultra-high performance liquid chromatography coupled with high resolution mass spectrometry was used to establish and compare mitochondrial lipidomes of brain and skeletal muscle from young (4-11 weeks old) and middle age (78 weeks old) healthy mice. In middle age the brain mitochondria had reduced levels of fatty acids, particularly polyunsaturated fatty acids, while skeletal muscle mitochondria had a decreased abundance of phosphatidylethanolamine, but a pronounced increase of triglyceride levels. Reduced levels of phosphatidylethanolamines are known to decrease mitochondrial membrane fluidity and are connected with accelerated ageing. In mitochondria from skeletal muscle we propose that increased age causes a metabolic shift in the conversion of diacylglycerol so that triglycerides predominate compared with phosphatidylethanolamines. This is the first time mitochondrial lipid content in normal healthy mammalian ageing brain and muscle has been catalogued in detail across all lipid classes. We identify distinct mitochondrial lipid signatures that change with age, revealing tissue-specific lipid pathways as possible targets to ameliorate ageing-related mitochondrial decline. Impact Journals 2017-03-21 Article PeerReviewed Pollard, Amelia K., Ortori, Catharine A., Stöger, Reinhard, Barrett, David A. and Chakrabarti, Lisa (2017) Mouse mitochondrial lipid composition is defined by age in brain and muscle. Aging, 9 (3). pp. 986-995. ISSN 1945-4589 mitochondria global lipidomics ageing mouse skeletal muscle brain http://www.aging-us.com/article/101204 doi:10.18632/aging.101204 doi:10.18632/aging.101204 |
| spellingShingle | mitochondria global lipidomics ageing mouse skeletal muscle brain Pollard, Amelia K. Ortori, Catharine A. Stöger, Reinhard Barrett, David A. Chakrabarti, Lisa Mouse mitochondrial lipid composition is defined by age in brain and muscle |
| title | Mouse mitochondrial lipid composition is defined by age in brain and muscle |
| title_full | Mouse mitochondrial lipid composition is defined by age in brain and muscle |
| title_fullStr | Mouse mitochondrial lipid composition is defined by age in brain and muscle |
| title_full_unstemmed | Mouse mitochondrial lipid composition is defined by age in brain and muscle |
| title_short | Mouse mitochondrial lipid composition is defined by age in brain and muscle |
| title_sort | mouse mitochondrial lipid composition is defined by age in brain and muscle |
| topic | mitochondria global lipidomics ageing mouse skeletal muscle brain |
| url | https://eprints.nottingham.ac.uk/41327/ https://eprints.nottingham.ac.uk/41327/ https://eprints.nottingham.ac.uk/41327/ |