A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut
Bambara groundnut (Vigna subterranea (L.) Verdc.) is an underutilised legume crop, which has long been recognised as a protein-rich and drought-tolerant crop, used extensively in Sub-Saharan Africa. The aim of the study was to identify quantitative trait loci (QTL) involved in agronomic and drought-...
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MDPI
2017
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| Online Access: | https://eprints.nottingham.ac.uk/40897/ |
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| author | Chai, Hui Ho, Wai Graham, Neil May, Sean Massawe, Festo Mayes, Sean |
| author_facet | Chai, Hui Ho, Wai Graham, Neil May, Sean Massawe, Festo Mayes, Sean |
| author_sort | Chai, Hui |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Bambara groundnut (Vigna subterranea (L.) Verdc.) is an underutilised legume crop, which has long been recognised as a protein-rich and drought-tolerant crop, used extensively in Sub-Saharan Africa. The aim of the study was to identify quantitative trait loci (QTL) involved in agronomic and drought-related traits using an expression marker-based genetic map based on major crop resources developed in soybean. The gene expression markers (GEMs) were generated at the (unmasked) probe-pair level after cross-hybridisation of bambara groundnut leaf RNA to the Affymetrix Soybean Genome GeneChip. A total of 753 markers grouped at an LOD (Logarithm of odds) of three, with 527 markers mapped into linkage groups. From this initial map, a spaced expression marker-based genetic map consisting of 13 linkage groups containing 218 GEMs, spanning 982.7 cM (centimorgan) of the bambara groundnut genome, was developed. Of the QTL detected, 46% were detected in both control and drought treatment populations, suggesting that they are the result of intrinsic trait differences between the parental lines used to construct the cross, with 31% detected in only one of the conditions. The present GEM map in bambara groundnut provides one technically feasible route for the translation of information and resources from major and model plant species to underutilised and resource-poor crops. |
| first_indexed | 2025-11-14T19:43:31Z |
| format | Article |
| id | nottingham-40897 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T21:03:20Z |
| publishDate | 2017 |
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| recordtype | eprints |
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| spelling | nottingham-408972025-09-09T13:49:52Z https://eprints.nottingham.ac.uk/40897/ A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut Chai, Hui Ho, Wai Graham, Neil May, Sean Massawe, Festo Mayes, Sean Bambara groundnut (Vigna subterranea (L.) Verdc.) is an underutilised legume crop, which has long been recognised as a protein-rich and drought-tolerant crop, used extensively in Sub-Saharan Africa. The aim of the study was to identify quantitative trait loci (QTL) involved in agronomic and drought-related traits using an expression marker-based genetic map based on major crop resources developed in soybean. The gene expression markers (GEMs) were generated at the (unmasked) probe-pair level after cross-hybridisation of bambara groundnut leaf RNA to the Affymetrix Soybean Genome GeneChip. A total of 753 markers grouped at an LOD (Logarithm of odds) of three, with 527 markers mapped into linkage groups. From this initial map, a spaced expression marker-based genetic map consisting of 13 linkage groups containing 218 GEMs, spanning 982.7 cM (centimorgan) of the bambara groundnut genome, was developed. Of the QTL detected, 46% were detected in both control and drought treatment populations, suggesting that they are the result of intrinsic trait differences between the parental lines used to construct the cross, with 31% detected in only one of the conditions. The present GEM map in bambara groundnut provides one technically feasible route for the translation of information and resources from major and model plant species to underutilised and resource-poor crops. MDPI 2017-02-22 Article PeerReviewed Chai, Hui, Ho, Wai, Graham, Neil, May, Sean, Massawe, Festo and Mayes, Sean (2017) A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut. Genes, 8 (3). p. 84. ISSN 2073-4425 Affymetrix GeneChip; cross-hybridisation; gene expression markers; quantitative trait loci http://www.mdpi.com/2073-4425/8/2/84 doi:10.3390/genes8020084 doi:10.3390/genes8020084 |
| spellingShingle | Affymetrix GeneChip; cross-hybridisation; gene expression markers; quantitative trait loci Chai, Hui Ho, Wai Graham, Neil May, Sean Massawe, Festo Mayes, Sean A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut |
| title | A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut |
| title_full | A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut |
| title_fullStr | A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut |
| title_full_unstemmed | A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut |
| title_short | A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut |
| title_sort | cross-species gene expression marker-based genetic map and qtl analysis in bambara groundnut |
| topic | Affymetrix GeneChip; cross-hybridisation; gene expression markers; quantitative trait loci |
| url | https://eprints.nottingham.ac.uk/40897/ https://eprints.nottingham.ac.uk/40897/ https://eprints.nottingham.ac.uk/40897/ |