³¹P magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral ATP metabolic rates at 3 T
Purpose: In vivo ³¹P MRS magnetization transfer (MT) provides a direct measure of neuronal activity at the metabolic level. This work aims to use functional ³¹P MRS-MT to investigate the change in cerebral ATP metabolic rates in healthy adults upon repeated visual stimuli. Methods: A magnetizati...
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| Format: | Article |
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Wiley
2017
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| Online Access: | https://eprints.nottingham.ac.uk/40872/ |
| _version_ | 1848796151952703488 |
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| author | Chen, Chen Stephenson, Mary C. Peters, Andrew Morris, Peter G. Francis, Susan T. Gowland, Penny A. |
| author_facet | Chen, Chen Stephenson, Mary C. Peters, Andrew Morris, Peter G. Francis, Susan T. Gowland, Penny A. |
| author_sort | Chen, Chen |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Purpose: In vivo ³¹P MRS magnetization transfer (MT) provides a direct measure of neuronal activity at the metabolic level. This work aims to use functional ³¹P MRS-MT to investigate the change in cerebral ATP metabolic rates in healthy adults upon repeated visual stimuli.
Methods: A magnetization saturation transfer sequence with narrowband selective saturation of γ-ATP was developed for ³¹P MT experiments at 3 T.
Results: Using progressive saturation of γ-ATP, the intrinsic T1 relaxation times of phosphocreatine (PCr) and inorganic phosphate (Pi) at 3 T were measured to be 5.1±0.8 s and 3.0±1.4 s, respectively. Using steady-state saturation of γ-ATP, a significant 24±14% and 11±7% increase in the forward creatine kinase (CK) pseudo-first-order reaction rate constant, k₁, was observed upon visual stimulation in the first and second cycles respectively of a paradigm consisting of 10min-rest followed by 10min-stimulation, with the measured baseline k₁ being 0.35±0.04 s⁻¹. No significant changes in forward ATP synthase (ATPase) reaction rate, PCr/γ- ATP, Pi/γ-ATP, and NAD(H)/γ-ATP ratios, or intracellular pH were detected upon stimulation.
Conclusion: This work demonstrates the potential of studying cerebral bioenergetics using functional ³¹P MRS-MT to determine the change in the forward CK reaction rate at 3 T. |
| first_indexed | 2025-11-14T19:43:26Z |
| format | Article |
| id | nottingham-40872 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:43:26Z |
| publishDate | 2017 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-408722020-05-04T18:38:03Z https://eprints.nottingham.ac.uk/40872/ ³¹P magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral ATP metabolic rates at 3 T Chen, Chen Stephenson, Mary C. Peters, Andrew Morris, Peter G. Francis, Susan T. Gowland, Penny A. Purpose: In vivo ³¹P MRS magnetization transfer (MT) provides a direct measure of neuronal activity at the metabolic level. This work aims to use functional ³¹P MRS-MT to investigate the change in cerebral ATP metabolic rates in healthy adults upon repeated visual stimuli. Methods: A magnetization saturation transfer sequence with narrowband selective saturation of γ-ATP was developed for ³¹P MT experiments at 3 T. Results: Using progressive saturation of γ-ATP, the intrinsic T1 relaxation times of phosphocreatine (PCr) and inorganic phosphate (Pi) at 3 T were measured to be 5.1±0.8 s and 3.0±1.4 s, respectively. Using steady-state saturation of γ-ATP, a significant 24±14% and 11±7% increase in the forward creatine kinase (CK) pseudo-first-order reaction rate constant, k₁, was observed upon visual stimulation in the first and second cycles respectively of a paradigm consisting of 10min-rest followed by 10min-stimulation, with the measured baseline k₁ being 0.35±0.04 s⁻¹. No significant changes in forward ATP synthase (ATPase) reaction rate, PCr/γ- ATP, Pi/γ-ATP, and NAD(H)/γ-ATP ratios, or intracellular pH were detected upon stimulation. Conclusion: This work demonstrates the potential of studying cerebral bioenergetics using functional ³¹P MRS-MT to determine the change in the forward CK reaction rate at 3 T. Wiley 2017-03-16 Article PeerReviewed Chen, Chen, Stephenson, Mary C., Peters, Andrew, Morris, Peter G., Francis, Susan T. and Gowland, Penny A. (2017) ³¹P magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral ATP metabolic rates at 3 T. Magnetic Resonance in Medicine, 79 (1). pp. 22-30. ISSN 1522-2594 Adenosine triphosphate (ATP) metabolism; Magnetization transfer; In vivo 31P magnetic resonance spectroscopy (MRS); Visual stimulation; Bioenergetics; Brain http://onlinelibrary.wiley.com/doi/10.1002/mrm.26663/full doi:10.1002/mrm.26663 doi:10.1002/mrm.26663 |
| spellingShingle | Adenosine triphosphate (ATP) metabolism; Magnetization transfer; In vivo 31P magnetic resonance spectroscopy (MRS); Visual stimulation; Bioenergetics; Brain Chen, Chen Stephenson, Mary C. Peters, Andrew Morris, Peter G. Francis, Susan T. Gowland, Penny A. ³¹P magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral ATP metabolic rates at 3 T |
| title | ³¹P magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral ATP metabolic rates at 3 T |
| title_full | ³¹P magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral ATP metabolic rates at 3 T |
| title_fullStr | ³¹P magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral ATP metabolic rates at 3 T |
| title_full_unstemmed | ³¹P magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral ATP metabolic rates at 3 T |
| title_short | ³¹P magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral ATP metabolic rates at 3 T |
| title_sort | ³¹p magnetization transfer magnetic resonance spectroscopy: assessing the activation induced change in cerebral atp metabolic rates at 3 t |
| topic | Adenosine triphosphate (ATP) metabolism; Magnetization transfer; In vivo 31P magnetic resonance spectroscopy (MRS); Visual stimulation; Bioenergetics; Brain |
| url | https://eprints.nottingham.ac.uk/40872/ https://eprints.nottingham.ac.uk/40872/ https://eprints.nottingham.ac.uk/40872/ |