ELISA in the multiplex era: potentials and pitfalls
Multiplex immunoassays confer several advantages over widely adopted singleplex immunoassays including increased efficiency at a reduced expense, greater output per sample volume ratios and higher throughput predicating more resolute, detailed diagnostics and facilitating personalised medicine. None...
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| Format: | Article |
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Wiley
2015
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| Online Access: | https://eprints.nottingham.ac.uk/40706/ |
| _version_ | 1848796121055363072 |
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| author | Tighe, Patrick J. Ryder, Richard R. Todd, Ian Fairclough, Lucy C. |
| author_facet | Tighe, Patrick J. Ryder, Richard R. Todd, Ian Fairclough, Lucy C. |
| author_sort | Tighe, Patrick J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Multiplex immunoassays confer several advantages over widely adopted singleplex immunoassays including increased efficiency at a reduced expense, greater output per sample volume ratios and higher throughput predicating more resolute, detailed diagnostics and facilitating personalised medicine. Nonetheless, to date, relatively few protein multiplex immunoassays have been validated for in vitro diagnostics in clinical/point-of-care settings. This review article will outline the challenges, which must be ameliorated prior to the widespread integration of multiplex immunoassays in clinical settings: (i) biomarker validation; (ii) standardisation of immunoassay design and quality control (calibration and quantification); (iii) availability, stability, specificity and cross-reactivity of reagents; (iv) assay automation and the use of validated algorithms for transformation of raw data into diagnostic results. A compendium of multiplex immunoassays applicable to in vitro diagnostics and a summary of the diagnostic products currently available commercially are included, along with an analysis of the relative states of development for each format (namely planar slide based, suspension and planar/microtitre plate based) with respect to the aforementioned issues. |
| first_indexed | 2025-11-14T19:42:56Z |
| format | Article |
| id | nottingham-40706 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:42:56Z |
| publishDate | 2015 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-407062020-05-04T17:06:24Z https://eprints.nottingham.ac.uk/40706/ ELISA in the multiplex era: potentials and pitfalls Tighe, Patrick J. Ryder, Richard R. Todd, Ian Fairclough, Lucy C. Multiplex immunoassays confer several advantages over widely adopted singleplex immunoassays including increased efficiency at a reduced expense, greater output per sample volume ratios and higher throughput predicating more resolute, detailed diagnostics and facilitating personalised medicine. Nonetheless, to date, relatively few protein multiplex immunoassays have been validated for in vitro diagnostics in clinical/point-of-care settings. This review article will outline the challenges, which must be ameliorated prior to the widespread integration of multiplex immunoassays in clinical settings: (i) biomarker validation; (ii) standardisation of immunoassay design and quality control (calibration and quantification); (iii) availability, stability, specificity and cross-reactivity of reagents; (iv) assay automation and the use of validated algorithms for transformation of raw data into diagnostic results. A compendium of multiplex immunoassays applicable to in vitro diagnostics and a summary of the diagnostic products currently available commercially are included, along with an analysis of the relative states of development for each format (namely planar slide based, suspension and planar/microtitre plate based) with respect to the aforementioned issues. Wiley 2015-04-20 Article PeerReviewed Tighe, Patrick J., Ryder, Richard R., Todd, Ian and Fairclough, Lucy C. (2015) ELISA in the multiplex era: potentials and pitfalls. Proteomics Clinical Applications, 9 (3-4). pp. 406-422. ISSN 1862-8354 ELISA In vitro diagnostics Multiplex immunoassay http://onlinelibrary.wiley.com/doi/10.1002/prca.201400130/abstract doi:10.1002/prca.201400130 doi:10.1002/prca.201400130 |
| spellingShingle | ELISA In vitro diagnostics Multiplex immunoassay Tighe, Patrick J. Ryder, Richard R. Todd, Ian Fairclough, Lucy C. ELISA in the multiplex era: potentials and pitfalls |
| title | ELISA in the multiplex era: potentials and pitfalls |
| title_full | ELISA in the multiplex era: potentials and pitfalls |
| title_fullStr | ELISA in the multiplex era: potentials and pitfalls |
| title_full_unstemmed | ELISA in the multiplex era: potentials and pitfalls |
| title_short | ELISA in the multiplex era: potentials and pitfalls |
| title_sort | elisa in the multiplex era: potentials and pitfalls |
| topic | ELISA In vitro diagnostics Multiplex immunoassay |
| url | https://eprints.nottingham.ac.uk/40706/ https://eprints.nottingham.ac.uk/40706/ https://eprints.nottingham.ac.uk/40706/ |