Negative regulation of violacein biosynthesis in Chromobacterium violaceum
In Chromobacteium violaceum, the purple pigment violacein is under positive regulation by the N-acylhomoserine lactone CviI/R quorum sensing system and negative regulation by an uncharacterized putative repressor. In this study we report that the biosynthesis of violacein is negatively controlled by...
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Frontiers
2017
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| Online Access: | https://eprints.nottingham.ac.uk/40663/ |
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| author | Devescovi, Giulia Kojic, Milan Covaceuszach, Sonia Cámara, Miguel Williams, Paul Bertani, Iris Subramoni, Sujatha Venturi, Vittorio |
| author_facet | Devescovi, Giulia Kojic, Milan Covaceuszach, Sonia Cámara, Miguel Williams, Paul Bertani, Iris Subramoni, Sujatha Venturi, Vittorio |
| author_sort | Devescovi, Giulia |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In Chromobacteium violaceum, the purple pigment violacein is under positive regulation by the N-acylhomoserine lactone CviI/R quorum sensing system and negative regulation by an uncharacterized putative repressor. In this study we report that the biosynthesis of violacein is negatively controlled by a novel repressor protein, VioS. The violacein operon is regulated negatively by VioS and positively by the CviI/R system in both C. violaceum and in a heterologous Escherichia coli genetic background. VioS does not regulate the CviI/R system and apart from violacein, VioS and quorum sensing regulate other phenotypes antagonistically. Quorum sensing regulated phenotypes in C. violaceum are therefore further regulated providing an additional level of control. |
| first_indexed | 2025-11-14T19:42:46Z |
| format | Article |
| id | nottingham-40663 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:42:46Z |
| publishDate | 2017 |
| publisher | Frontiers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-406632020-05-04T18:34:10Z https://eprints.nottingham.ac.uk/40663/ Negative regulation of violacein biosynthesis in Chromobacterium violaceum Devescovi, Giulia Kojic, Milan Covaceuszach, Sonia Cámara, Miguel Williams, Paul Bertani, Iris Subramoni, Sujatha Venturi, Vittorio In Chromobacteium violaceum, the purple pigment violacein is under positive regulation by the N-acylhomoserine lactone CviI/R quorum sensing system and negative regulation by an uncharacterized putative repressor. In this study we report that the biosynthesis of violacein is negatively controlled by a novel repressor protein, VioS. The violacein operon is regulated negatively by VioS and positively by the CviI/R system in both C. violaceum and in a heterologous Escherichia coli genetic background. VioS does not regulate the CviI/R system and apart from violacein, VioS and quorum sensing regulate other phenotypes antagonistically. Quorum sensing regulated phenotypes in C. violaceum are therefore further regulated providing an additional level of control. Frontiers 2017-02-20 Article PeerReviewed Devescovi, Giulia, Kojic, Milan, Covaceuszach, Sonia, Cámara, Miguel, Williams, Paul, Bertani, Iris, Subramoni, Sujatha and Venturi, Vittorio (2017) Negative regulation of violacein biosynthesis in Chromobacterium violaceum. Frontiers in Microbiology, 8 . 349/1-349/37. ISSN 1664-302X Chromobacterium violaceum VioS CviI/R quorum sensing regulation Violacein Chitinase activity Protease activity http://journal.frontiersin.org/article/10.3389/fmicb.2017.00349/abstract doi:10.3389/fmicb.2017.00349 doi:10.3389/fmicb.2017.00349 |
| spellingShingle | Chromobacterium violaceum VioS CviI/R quorum sensing regulation Violacein Chitinase activity Protease activity Devescovi, Giulia Kojic, Milan Covaceuszach, Sonia Cámara, Miguel Williams, Paul Bertani, Iris Subramoni, Sujatha Venturi, Vittorio Negative regulation of violacein biosynthesis in Chromobacterium violaceum |
| title | Negative regulation of violacein biosynthesis in Chromobacterium violaceum |
| title_full | Negative regulation of violacein biosynthesis in Chromobacterium violaceum |
| title_fullStr | Negative regulation of violacein biosynthesis in Chromobacterium violaceum |
| title_full_unstemmed | Negative regulation of violacein biosynthesis in Chromobacterium violaceum |
| title_short | Negative regulation of violacein biosynthesis in Chromobacterium violaceum |
| title_sort | negative regulation of violacein biosynthesis in chromobacterium violaceum |
| topic | Chromobacterium violaceum VioS CviI/R quorum sensing regulation Violacein Chitinase activity Protease activity |
| url | https://eprints.nottingham.ac.uk/40663/ https://eprints.nottingham.ac.uk/40663/ https://eprints.nottingham.ac.uk/40663/ |