Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection
Although microRNAs (miRNAs) play an important role in liver homeostasis, the extent to which they can be altered by Toxoplasma gondii infection is unknown. Here, we utilized small RNA sequencing and bioinformatic analyses to characterize miRNA expression profiles in the liver of domestic cats at 7 d...
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Impact Journals
2017
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| Online Access: | https://eprints.nottingham.ac.uk/40648/ |
| _version_ | 1848796106759077888 |
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| author | Cong, Wei Zhang, Xiao-Xuan He, Jun-Jun Li, Fa-Cai Elsheikha, Hany M. Zhu, Xing-Quan |
| author_facet | Cong, Wei Zhang, Xiao-Xuan He, Jun-Jun Li, Fa-Cai Elsheikha, Hany M. Zhu, Xing-Quan |
| author_sort | Cong, Wei |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Although microRNAs (miRNAs) play an important role in liver homeostasis, the extent to which they can be altered by Toxoplasma gondii infection is unknown. Here, we utilized small RNA sequencing and bioinformatic analyses to characterize miRNA expression profiles in the liver of domestic cats at 7 days after oral infection with T. gondii (Type II) strain. A total of 384 miRNAs were identified and 82 were differentially expressed, of which 33 were up-regulated and 49 down-regulated. Also, 5690 predicted host gene targets for the differentially expressed miRNAs were identified using the bioinformatic algorithm miRanda. Gene ontology analysis revealed that the predicted gene targets of the dysregulated miRNAs were significantly enriched in apoptosis. Kyoto Encyclopedia of Genes and Genomes analysis showed that the predicted gene targets were involved in several pathways, including acute myeloid leukemia, central carbon metabolism in cancer, choline metabolism in cancer, estrogen signaling pathway, fatty acid degradation, lysosome, nucleotide excision repair, progesterone-mediated oocyte maturation, and VEGF signaling pathway. The expression level of 6 upregulated miRNAs (mmu-miR-21a-5p, mmu-miR-20a-5p, mmu-miR-17-5p, mmu-miR-30e-3p, mmu-miR-142a-3p, and mmu-miR-106b-3p) was confirmed by stem-loop quantitative reverse transcription PCR, which yielded results consistent with the sequencing data. These findings expand our understanding of the regulatory mechanisms of miRNAs underlying T. gondii pathogenesis and contribute new database information on cat miRNAs, opening a new perspective on the prevention and treatment of T. gondii infection. |
| first_indexed | 2025-11-14T19:42:43Z |
| format | Article |
| id | nottingham-40648 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:42:43Z |
| publishDate | 2017 |
| publisher | Impact Journals |
| recordtype | eprints |
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| spelling | nottingham-406482020-05-04T18:37:22Z https://eprints.nottingham.ac.uk/40648/ Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection Cong, Wei Zhang, Xiao-Xuan He, Jun-Jun Li, Fa-Cai Elsheikha, Hany M. Zhu, Xing-Quan Although microRNAs (miRNAs) play an important role in liver homeostasis, the extent to which they can be altered by Toxoplasma gondii infection is unknown. Here, we utilized small RNA sequencing and bioinformatic analyses to characterize miRNA expression profiles in the liver of domestic cats at 7 days after oral infection with T. gondii (Type II) strain. A total of 384 miRNAs were identified and 82 were differentially expressed, of which 33 were up-regulated and 49 down-regulated. Also, 5690 predicted host gene targets for the differentially expressed miRNAs were identified using the bioinformatic algorithm miRanda. Gene ontology analysis revealed that the predicted gene targets of the dysregulated miRNAs were significantly enriched in apoptosis. Kyoto Encyclopedia of Genes and Genomes analysis showed that the predicted gene targets were involved in several pathways, including acute myeloid leukemia, central carbon metabolism in cancer, choline metabolism in cancer, estrogen signaling pathway, fatty acid degradation, lysosome, nucleotide excision repair, progesterone-mediated oocyte maturation, and VEGF signaling pathway. The expression level of 6 upregulated miRNAs (mmu-miR-21a-5p, mmu-miR-20a-5p, mmu-miR-17-5p, mmu-miR-30e-3p, mmu-miR-142a-3p, and mmu-miR-106b-3p) was confirmed by stem-loop quantitative reverse transcription PCR, which yielded results consistent with the sequencing data. These findings expand our understanding of the regulatory mechanisms of miRNAs underlying T. gondii pathogenesis and contribute new database information on cat miRNAs, opening a new perspective on the prevention and treatment of T. gondii infection. Impact Journals 2017-03-10 Article PeerReviewed Cong, Wei, Zhang, Xiao-Xuan, He, Jun-Jun, Li, Fa-Cai, Elsheikha, Hany M. and Zhu, Xing-Quan (2017) Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection. Oncotarget, 8 . pp. 25599-25611. ISSN 1949-2553 Toxoplasma gondii Domestic cat Liver MircoRNA RNA-seq http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=16108&author-preview=cfg doi:10.18632/oncotarget.16108 doi:10.18632/oncotarget.16108 |
| spellingShingle | Toxoplasma gondii Domestic cat Liver MircoRNA RNA-seq Cong, Wei Zhang, Xiao-Xuan He, Jun-Jun Li, Fa-Cai Elsheikha, Hany M. Zhu, Xing-Quan Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection |
| title | Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection |
| title_full | Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection |
| title_fullStr | Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection |
| title_full_unstemmed | Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection |
| title_short | Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection |
| title_sort | global mirna expression profiling of domestic cat livers following acute toxoplasma gondii infection |
| topic | Toxoplasma gondii Domestic cat Liver MircoRNA RNA-seq |
| url | https://eprints.nottingham.ac.uk/40648/ https://eprints.nottingham.ac.uk/40648/ https://eprints.nottingham.ac.uk/40648/ |