Sterol regulatory element binding protein-1 (SREBP1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer

Introduction: Women with polycystic ovary syndrome (PCOS) have a 3-fold higher risk of endometrial cancer (EC). Insulin resistance and hyperlipidaemia may be pertinent factors in the pathogenesis of both conditions. The aim of this study was to investigate endometrial Sterol Regulatory Element Bindi...

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Main Authors: Shafiee, Mohamad Nasir, Mongan, Nigel P., Seedhouse, Claire, Chapman, Caroline, Deen, Suha, Abu, Jafaru, Atiomo, William
Format: Article
Published: Wiley 2017
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Online Access:https://eprints.nottingham.ac.uk/40461/
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author Shafiee, Mohamad Nasir
Mongan, Nigel P.
Seedhouse, Claire
Chapman, Caroline
Deen, Suha
Abu, Jafaru
Atiomo, William
author_facet Shafiee, Mohamad Nasir
Mongan, Nigel P.
Seedhouse, Claire
Chapman, Caroline
Deen, Suha
Abu, Jafaru
Atiomo, William
author_sort Shafiee, Mohamad Nasir
building Nottingham Research Data Repository
collection Online Access
description Introduction: Women with polycystic ovary syndrome (PCOS) have a 3-fold higher risk of endometrial cancer (EC). Insulin resistance and hyperlipidaemia may be pertinent factors in the pathogenesis of both conditions. The aim of this study was to investigate endometrial Sterol Regulatory Element Binding Protein-1 gene (SREBP1) expression in PCOS and EC endometrium, and to correlate endometrial SREBP1 expression with serum lipid profiles. Material and methods: A cross-sectional study was performed at Nottingham University Hospital, United Kingdom. A total of 102 women (PCOS, EC and controls; 34 participants in each group) were recruited. Clinical and biochemical assessments were performed before endometrial biopsies were obtained from all participants. Taqman real-time PCR for endometrial SREBP1 and its systemic protein expression were analysed. Results: The BMI of women with PCOS (29.28 (±2.91) kg/m2) and controls (28.58 (±2.62) kg/m2) was not significantly different. Women with EC had a higher mean BMI (32.22 (±5.70) kg/m2). SREBP1 gene expression was significantly increased in PCOS and EC endometrium compared to controls (p<0.0001). SREBP1 gene expression was positively correlated with BMI (r=0.017, p=0.921) and waist-hip ratio (r=0.023, p=0.544) in PCOS, but this was not statistically significant. Similarly, statistically insignificant positive correlations were found between endometrial SREBP1 gene expression and BMI in EC (r=0.643, p=0.06) and waist-hip ratio (r=0.096, p=0.073). SREBP1 expression was significantly positively correlated with triglyceride in both PCOS and EC (p= 0.028 and p=0.027). Quantitative serum SREBP1 correlated with endometrial gene expression (p<0.05). Conclusions: SREBP1 gene expression is significantly increased in the endometrium of PCOS and EC women compared with controls and positively correlates with serum triglyceride in both PCOS and EC.
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spelling nottingham-404612024-08-15T15:22:27Z https://eprints.nottingham.ac.uk/40461/ Sterol regulatory element binding protein-1 (SREBP1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer Shafiee, Mohamad Nasir Mongan, Nigel P. Seedhouse, Claire Chapman, Caroline Deen, Suha Abu, Jafaru Atiomo, William Introduction: Women with polycystic ovary syndrome (PCOS) have a 3-fold higher risk of endometrial cancer (EC). Insulin resistance and hyperlipidaemia may be pertinent factors in the pathogenesis of both conditions. The aim of this study was to investigate endometrial Sterol Regulatory Element Binding Protein-1 gene (SREBP1) expression in PCOS and EC endometrium, and to correlate endometrial SREBP1 expression with serum lipid profiles. Material and methods: A cross-sectional study was performed at Nottingham University Hospital, United Kingdom. A total of 102 women (PCOS, EC and controls; 34 participants in each group) were recruited. Clinical and biochemical assessments were performed before endometrial biopsies were obtained from all participants. Taqman real-time PCR for endometrial SREBP1 and its systemic protein expression were analysed. Results: The BMI of women with PCOS (29.28 (±2.91) kg/m2) and controls (28.58 (±2.62) kg/m2) was not significantly different. Women with EC had a higher mean BMI (32.22 (±5.70) kg/m2). SREBP1 gene expression was significantly increased in PCOS and EC endometrium compared to controls (p<0.0001). SREBP1 gene expression was positively correlated with BMI (r=0.017, p=0.921) and waist-hip ratio (r=0.023, p=0.544) in PCOS, but this was not statistically significant. Similarly, statistically insignificant positive correlations were found between endometrial SREBP1 gene expression and BMI in EC (r=0.643, p=0.06) and waist-hip ratio (r=0.096, p=0.073). SREBP1 expression was significantly positively correlated with triglyceride in both PCOS and EC (p= 0.028 and p=0.027). Quantitative serum SREBP1 correlated with endometrial gene expression (p<0.05). Conclusions: SREBP1 gene expression is significantly increased in the endometrium of PCOS and EC women compared with controls and positively correlates with serum triglyceride in both PCOS and EC. Wiley 2017-04-20 Article PeerReviewed Shafiee, Mohamad Nasir, Mongan, Nigel P., Seedhouse, Claire, Chapman, Caroline, Deen, Suha, Abu, Jafaru and Atiomo, William (2017) Sterol regulatory element binding protein-1 (SREBP1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer. Acta Obstetricia et Gynecologica Scandinavica, 96 (5). pp. 556-562. ISSN 1600-0412 Endometrial cancer PCOS SREBP1 Regulatory Element Binding Protein-1 http://onlinelibrary.wiley.com/doi/10.1111/aogs.13106/abstract doi:10.1111/aogs.13106 doi:10.1111/aogs.13106
spellingShingle Endometrial cancer
PCOS
SREBP1
Regulatory Element Binding Protein-1
Shafiee, Mohamad Nasir
Mongan, Nigel P.
Seedhouse, Claire
Chapman, Caroline
Deen, Suha
Abu, Jafaru
Atiomo, William
Sterol regulatory element binding protein-1 (SREBP1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer
title Sterol regulatory element binding protein-1 (SREBP1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer
title_full Sterol regulatory element binding protein-1 (SREBP1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer
title_fullStr Sterol regulatory element binding protein-1 (SREBP1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer
title_full_unstemmed Sterol regulatory element binding protein-1 (SREBP1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer
title_short Sterol regulatory element binding protein-1 (SREBP1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer
title_sort sterol regulatory element binding protein-1 (srebp1) gene expression is similarly increased in polycystic ovary syndrome and endometrial cancer
topic Endometrial cancer
PCOS
SREBP1
Regulatory Element Binding Protein-1
url https://eprints.nottingham.ac.uk/40461/
https://eprints.nottingham.ac.uk/40461/
https://eprints.nottingham.ac.uk/40461/