Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals
HIV-1 infected patients who acquire HCV infection have higher rates of chronicity and liver disease progression than patients with HCV mono-infection. Understanding early events in this pathogenic process is important. We applied single genome sequencing of the E1 to NS3 regions and viral pseudotype...
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| Format: | Article |
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/40451/ |
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| author | Ferns, R. Bridget Tarr, Alexander W. Hue, Stephane Urbanowicz, Richard A. McClure, C. Patrick Gilson, Richard Ball, Jonathan K. Nastouli, Eleni Garson, Jeremy A. Pillay, Deenan |
| author_facet | Ferns, R. Bridget Tarr, Alexander W. Hue, Stephane Urbanowicz, Richard A. McClure, C. Patrick Gilson, Richard Ball, Jonathan K. Nastouli, Eleni Garson, Jeremy A. Pillay, Deenan |
| author_sort | Ferns, R. Bridget |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | HIV-1 infected patients who acquire HCV infection have higher rates of chronicity and liver disease progression than patients with HCV mono-infection. Understanding early events in this pathogenic process is important. We applied single genome sequencing of the E1 to NS3 regions and viral pseudotype neutralization assays to explore the consequences of viral quasispecies evolution from pre-seroconversion to chronicity in four co-infected individuals (mean follow up 566 days). We observed that one to three founder viruses were transmitted. Relatively low viral sequence diversity, possibly related to an impaired immune response, due to HIV infection was observed in three patients. However, the fourth patient, after an early purifying selection displayed increasing E2 sequence evolution, possibly related to being on suppressive antiretroviral therapy. Viral pseudotypes generated from HCV variants showed relative resistance to neutralization by autologous plasma but not to plasma collected from later time points, confirming ongoing virus escape from antibody neutralization. |
| first_indexed | 2025-11-14T19:41:58Z |
| format | Article |
| id | nottingham-40451 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:41:58Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-404512020-05-04T17:41:09Z https://eprints.nottingham.ac.uk/40451/ Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals Ferns, R. Bridget Tarr, Alexander W. Hue, Stephane Urbanowicz, Richard A. McClure, C. Patrick Gilson, Richard Ball, Jonathan K. Nastouli, Eleni Garson, Jeremy A. Pillay, Deenan HIV-1 infected patients who acquire HCV infection have higher rates of chronicity and liver disease progression than patients with HCV mono-infection. Understanding early events in this pathogenic process is important. We applied single genome sequencing of the E1 to NS3 regions and viral pseudotype neutralization assays to explore the consequences of viral quasispecies evolution from pre-seroconversion to chronicity in four co-infected individuals (mean follow up 566 days). We observed that one to three founder viruses were transmitted. Relatively low viral sequence diversity, possibly related to an impaired immune response, due to HIV infection was observed in three patients. However, the fourth patient, after an early purifying selection displayed increasing E2 sequence evolution, possibly related to being on suppressive antiretroviral therapy. Viral pseudotypes generated from HCV variants showed relative resistance to neutralization by autologous plasma but not to plasma collected from later time points, confirming ongoing virus escape from antibody neutralization. Elsevier 2016-03-21 Article PeerReviewed Ferns, R. Bridget, Tarr, Alexander W., Hue, Stephane, Urbanowicz, Richard A., McClure, C. Patrick, Gilson, Richard, Ball, Jonathan K., Nastouli, Eleni, Garson, Jeremy A. and Pillay, Deenan (2016) Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals. Virology, 492 . pp. 213-224. ISSN 0042-6822 HCV quasispecies; HIV-1 co-infection; Single genome sequencing; Founder virus; HCV pseudotype neutralization; Antiretroviral therapy http://www.sciencedirect.com/science/article/pii/S0042682216000465 doi:10.1016/j.virol.2016.02.003 doi:10.1016/j.virol.2016.02.003 |
| spellingShingle | HCV quasispecies; HIV-1 co-infection; Single genome sequencing; Founder virus; HCV pseudotype neutralization; Antiretroviral therapy Ferns, R. Bridget Tarr, Alexander W. Hue, Stephane Urbanowicz, Richard A. McClure, C. Patrick Gilson, Richard Ball, Jonathan K. Nastouli, Eleni Garson, Jeremy A. Pillay, Deenan Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals |
| title | Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals |
| title_full | Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals |
| title_fullStr | Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals |
| title_full_unstemmed | Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals |
| title_short | Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals |
| title_sort | hepatitis c virus quasispecies and pseudotype analysis from acute infection to chronicity in hiv-1 co-infected individuals |
| topic | HCV quasispecies; HIV-1 co-infection; Single genome sequencing; Founder virus; HCV pseudotype neutralization; Antiretroviral therapy |
| url | https://eprints.nottingham.ac.uk/40451/ https://eprints.nottingham.ac.uk/40451/ https://eprints.nottingham.ac.uk/40451/ |