Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos

Despite ongoing research in a number of species, the efficiency of embryo production by nuclear transfer remains low. Incomplete epigenetic reprogramming of the nucleus introduced in the recipient oocyte is one factor proposed to limit the success of this technique. Nonetheless, knowledge of reprogr...

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Main Authors: Sepulveda-Rincon, Lessly P., Solanas, Edgar del Llano, Serrano-Revuelta, Elisa, Ruddick, Lydia, Maalouf, Walid E., Beaujean, Nathalie
Format: Article
Published: Elsevier 2016
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Online Access:https://eprints.nottingham.ac.uk/40374/
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author Sepulveda-Rincon, Lessly P.
Solanas, Edgar del Llano
Serrano-Revuelta, Elisa
Ruddick, Lydia
Maalouf, Walid E.
Beaujean, Nathalie
author_facet Sepulveda-Rincon, Lessly P.
Solanas, Edgar del Llano
Serrano-Revuelta, Elisa
Ruddick, Lydia
Maalouf, Walid E.
Beaujean, Nathalie
author_sort Sepulveda-Rincon, Lessly P.
building Nottingham Research Data Repository
collection Online Access
description Despite ongoing research in a number of species, the efficiency of embryo production by nuclear transfer remains low. Incomplete epigenetic reprogramming of the nucleus introduced in the recipient oocyte is one factor proposed to limit the success of this technique. Nonetheless, knowledge of reprogramming factors has increased—thanks to comparative studies on reprogramming of the paternal genome brought by sperm on fertilization—and will be reviewed here. Another valuable model of reprogramming is the one obtained in the absence of sperm fertilization through artificial activation—the parthenote—and will also be introduced. Altogether the objective of this review is to have a better understanding on the mechanisms responsible for the resistance to reprogramming, not only because it could improve embryonic development but also as it could benefit therapeutic reprogramming research.
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spelling nottingham-403742020-05-04T17:54:09Z https://eprints.nottingham.ac.uk/40374/ Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos Sepulveda-Rincon, Lessly P. Solanas, Edgar del Llano Serrano-Revuelta, Elisa Ruddick, Lydia Maalouf, Walid E. Beaujean, Nathalie Despite ongoing research in a number of species, the efficiency of embryo production by nuclear transfer remains low. Incomplete epigenetic reprogramming of the nucleus introduced in the recipient oocyte is one factor proposed to limit the success of this technique. Nonetheless, knowledge of reprogramming factors has increased—thanks to comparative studies on reprogramming of the paternal genome brought by sperm on fertilization—and will be reviewed here. Another valuable model of reprogramming is the one obtained in the absence of sperm fertilization through artificial activation—the parthenote—and will also be introduced. Altogether the objective of this review is to have a better understanding on the mechanisms responsible for the resistance to reprogramming, not only because it could improve embryonic development but also as it could benefit therapeutic reprogramming research. Elsevier 2016-07-01 Article PeerReviewed Sepulveda-Rincon, Lessly P., Solanas, Edgar del Llano, Serrano-Revuelta, Elisa, Ruddick, Lydia, Maalouf, Walid E. and Beaujean, Nathalie (2016) Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos. Theriogenology, 86 (1). pp. 91-98. ISSN 1879-3231 Oocyte; Nuclear transfer; Embryonic genome activation; Histones posttranslational modifications; DNA methylation http://dx.doi.org/10.1016/j.theriogenology.2016.04.022 doi:10.1016/j.theriogenology.2016.04.022 doi:10.1016/j.theriogenology.2016.04.022
spellingShingle Oocyte; Nuclear transfer; Embryonic genome activation; Histones posttranslational modifications; DNA methylation
Sepulveda-Rincon, Lessly P.
Solanas, Edgar del Llano
Serrano-Revuelta, Elisa
Ruddick, Lydia
Maalouf, Walid E.
Beaujean, Nathalie
Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos
title Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos
title_full Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos
title_fullStr Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos
title_full_unstemmed Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos
title_short Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos
title_sort early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos
topic Oocyte; Nuclear transfer; Embryonic genome activation; Histones posttranslational modifications; DNA methylation
url https://eprints.nottingham.ac.uk/40374/
https://eprints.nottingham.ac.uk/40374/
https://eprints.nottingham.ac.uk/40374/