The MYB36 transcription factor orchestrates Casparian strip formation
The endodermis in roots acts as a selectivity filter for nutrient and water transport essential for growth and development. This selectivity is enabled by the formation of lignin-based Casparian strips. Casparian strip formation is initiated by the localization of the Casparian strip domain proteins...
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| Format: | Article |
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National Academy of Sciences
2015
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| Online Access: | https://eprints.nottingham.ac.uk/40112/ |
| _version_ | 1848795987377651712 |
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| author | Kamiya, Takehiro Borghi, Monica Wang, Peng Danku, John Kalmbach, Lothar Hosmani, Prashant S. Naseer, Sadaf Fujiwara, Toru Geldner, Niko Salt, David E. |
| author_facet | Kamiya, Takehiro Borghi, Monica Wang, Peng Danku, John Kalmbach, Lothar Hosmani, Prashant S. Naseer, Sadaf Fujiwara, Toru Geldner, Niko Salt, David E. |
| author_sort | Kamiya, Takehiro |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The endodermis in roots acts as a selectivity filter for nutrient and water transport essential for growth and development. This selectivity is enabled by the formation of lignin-based Casparian strips. Casparian strip formation is initiated by the localization of the Casparian strip domain proteins (CASPs) in the plasma membrane, at the site where the Casparian strip will form. Localized CASPs recruit Peroxidase 64 (PER64), a Respiratory Burst Oxidase Homolog F, and Enhanced Suberin 1 (ESB1), a dirigent-like protein, to assemble the lignin polymerization machinery. However, the factors that control both expression of the genes encoding this biosynthetic machinery and its localization to the Casparian strip formation site remain unknown. Here, we identify the transcription factor, MYB36, essential for Casparian strip formation. MYB36 directly and positively regulates the expression of the Casparian strip genes CASP1, PER64, and ESB1. Casparian strips are absent in plants lacking a functional MYB36 and are replaced by ectopic lignin-like material in the corners of endodermal cells. The barrier function of Casparian strips in these plants is also disrupted. Significantly, ectopic expression of MYB36 in the cortex is sufficient to reprogram these cells to start expressing CASP1–GFP, correctly localize the CASP1–GFP protein to form a Casparian strip domain, and deposit a Casparian strip-like structure in the cell wall at this location. These results demonstrate that MYB36 is controlling expression of the machinery required to locally polymerize lignin in a fine band in the cell wall for the formation of the Casparian strip. |
| first_indexed | 2025-11-14T19:40:49Z |
| format | Article |
| id | nottingham-40112 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:40:49Z |
| publishDate | 2015 |
| publisher | National Academy of Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-401122020-05-04T17:14:54Z https://eprints.nottingham.ac.uk/40112/ The MYB36 transcription factor orchestrates Casparian strip formation Kamiya, Takehiro Borghi, Monica Wang, Peng Danku, John Kalmbach, Lothar Hosmani, Prashant S. Naseer, Sadaf Fujiwara, Toru Geldner, Niko Salt, David E. The endodermis in roots acts as a selectivity filter for nutrient and water transport essential for growth and development. This selectivity is enabled by the formation of lignin-based Casparian strips. Casparian strip formation is initiated by the localization of the Casparian strip domain proteins (CASPs) in the plasma membrane, at the site where the Casparian strip will form. Localized CASPs recruit Peroxidase 64 (PER64), a Respiratory Burst Oxidase Homolog F, and Enhanced Suberin 1 (ESB1), a dirigent-like protein, to assemble the lignin polymerization machinery. However, the factors that control both expression of the genes encoding this biosynthetic machinery and its localization to the Casparian strip formation site remain unknown. Here, we identify the transcription factor, MYB36, essential for Casparian strip formation. MYB36 directly and positively regulates the expression of the Casparian strip genes CASP1, PER64, and ESB1. Casparian strips are absent in plants lacking a functional MYB36 and are replaced by ectopic lignin-like material in the corners of endodermal cells. The barrier function of Casparian strips in these plants is also disrupted. Significantly, ectopic expression of MYB36 in the cortex is sufficient to reprogram these cells to start expressing CASP1–GFP, correctly localize the CASP1–GFP protein to form a Casparian strip domain, and deposit a Casparian strip-like structure in the cell wall at this location. These results demonstrate that MYB36 is controlling expression of the machinery required to locally polymerize lignin in a fine band in the cell wall for the formation of the Casparian strip. National Academy of Sciences 2015-08-18 Article PeerReviewed Kamiya, Takehiro, Borghi, Monica, Wang, Peng, Danku, John, Kalmbach, Lothar, Hosmani, Prashant S., Naseer, Sadaf, Fujiwara, Toru, Geldner, Niko and Salt, David E. (2015) The MYB36 transcription factor orchestrates Casparian strip formation. Proceedings of the National Academy of Sciences, 112 (33). pp. 10533-10538. ISSN 1091-6490 Casparian strip transcription factor lignin endodermis cell wall http://www.pnas.org/content/112/33/10533 doi:10.1073/pnas.1507691112 doi:10.1073/pnas.1507691112 |
| spellingShingle | Casparian strip transcription factor lignin endodermis cell wall Kamiya, Takehiro Borghi, Monica Wang, Peng Danku, John Kalmbach, Lothar Hosmani, Prashant S. Naseer, Sadaf Fujiwara, Toru Geldner, Niko Salt, David E. The MYB36 transcription factor orchestrates Casparian strip formation |
| title | The MYB36 transcription factor orchestrates Casparian
strip formation |
| title_full | The MYB36 transcription factor orchestrates Casparian
strip formation |
| title_fullStr | The MYB36 transcription factor orchestrates Casparian
strip formation |
| title_full_unstemmed | The MYB36 transcription factor orchestrates Casparian
strip formation |
| title_short | The MYB36 transcription factor orchestrates Casparian
strip formation |
| title_sort | myb36 transcription factor orchestrates casparian
strip formation |
| topic | Casparian strip transcription factor lignin endodermis cell wall |
| url | https://eprints.nottingham.ac.uk/40112/ https://eprints.nottingham.ac.uk/40112/ https://eprints.nottingham.ac.uk/40112/ |