Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes
Kinetochores are multiprotein complexes that couple eukaryotic chromosomes to the mitotic spindle to ensure proper segregation. The model for kinetochore assembly is conserved between humans and yeast, and homologues of several components are widely distributed in eukaryotes, but key components are...
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| Format: | Article |
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Rockefeller University Press
2016
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| Online Access: | https://eprints.nottingham.ac.uk/40078/ |
| _version_ | 1848795980010356736 |
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| author | D’Archivio, Simon Wickstead, Bill |
| author_facet | D’Archivio, Simon Wickstead, Bill |
| author_sort | D’Archivio, Simon |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Kinetochores are multiprotein complexes that couple eukaryotic chromosomes to the mitotic spindle to ensure proper segregation. The model for kinetochore assembly is conserved between humans and yeast, and homologues of several components are widely distributed in eukaryotes, but key components are absent in some lineages. The recent discovery in a lineage of protozoa called kinetoplastids of unconventional kinetochores with no apparent homology to model organisms suggests that more than one system for eukaryotic chromosome segregation may exist. In this study, we report a new family of proteins distantly related to outer kinetochore proteins Ndc80 and Nuf2. The family member in kinetoplastids, KKT-interacting protein 1 (KKIP1), associates with the kinetochore, and its depletion causes severe defects in karyokinesis, loss of individual chromosomes, and gross defects in spindle assembly or stability. Immunopurification of KKIP1 from stabilized kinetochores identifies six further components, which form part of a trypanosome outer kinetochore complex. These findings suggest that kinetochores in organisms such as kinetoplastids are built from a divergent, but not ancestrally distinct, set of components and that Ndc80/Nuf2-like proteins are universal in eukaryotic division. |
| first_indexed | 2025-11-14T19:40:42Z |
| format | Article |
| id | nottingham-40078 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:40:42Z |
| publishDate | 2016 |
| publisher | Rockefeller University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-400782020-05-04T18:24:04Z https://eprints.nottingham.ac.uk/40078/ Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes D’Archivio, Simon Wickstead, Bill Kinetochores are multiprotein complexes that couple eukaryotic chromosomes to the mitotic spindle to ensure proper segregation. The model for kinetochore assembly is conserved between humans and yeast, and homologues of several components are widely distributed in eukaryotes, but key components are absent in some lineages. The recent discovery in a lineage of protozoa called kinetoplastids of unconventional kinetochores with no apparent homology to model organisms suggests that more than one system for eukaryotic chromosome segregation may exist. In this study, we report a new family of proteins distantly related to outer kinetochore proteins Ndc80 and Nuf2. The family member in kinetoplastids, KKT-interacting protein 1 (KKIP1), associates with the kinetochore, and its depletion causes severe defects in karyokinesis, loss of individual chromosomes, and gross defects in spindle assembly or stability. Immunopurification of KKIP1 from stabilized kinetochores identifies six further components, which form part of a trypanosome outer kinetochore complex. These findings suggest that kinetochores in organisms such as kinetoplastids are built from a divergent, but not ancestrally distinct, set of components and that Ndc80/Nuf2-like proteins are universal in eukaryotic division. Rockefeller University Press 2016-12-29 Article PeerReviewed D’Archivio, Simon and Wickstead, Bill (2016) Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes. Journal of Cell Biology . ISSN 1540-8140 http://dx.doi.org/10.1083/jcb.201608043 doi:10.1083/jcb.201608043 doi:10.1083/jcb.201608043 |
| spellingShingle | D’Archivio, Simon Wickstead, Bill Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes |
| title | Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes |
| title_full | Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes |
| title_fullStr | Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes |
| title_full_unstemmed | Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes |
| title_short | Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes |
| title_sort | trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes |
| url | https://eprints.nottingham.ac.uk/40078/ https://eprints.nottingham.ac.uk/40078/ https://eprints.nottingham.ac.uk/40078/ |