Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels
Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated tha...
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| Format: | Article |
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/39999/ |
| _version_ | 1848795962846216192 |
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| author | Bilverstone, T.W. Kinsmore, N.L. Minton, Nigel P. Kuehne, Sarah A. |
| author_facet | Bilverstone, T.W. Kinsmore, N.L. Minton, Nigel P. Kuehne, Sarah A. |
| author_sort | Bilverstone, T.W. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity. |
| first_indexed | 2025-11-14T19:40:25Z |
| format | Article |
| id | nottingham-39999 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:40:25Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-399992020-05-04T18:30:23Z https://eprints.nottingham.ac.uk/39999/ Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels Bilverstone, T.W. Kinsmore, N.L. Minton, Nigel P. Kuehne, Sarah A. Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity. Elsevier 2017-01-17 Article PeerReviewed Bilverstone, T.W., Kinsmore, N.L., Minton, Nigel P. and Kuehne, Sarah A. (2017) Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels. Anaerobe, 44 . pp. 51-54. ISSN 1095-8274 C. difficile; PaLoc; Binary toxin; CdtR; Virulence http://www.sciencedirect.com/science/article/pii/S1075996417300173 doi:10.1016/j.anaerobe.2017.01.009 doi:10.1016/j.anaerobe.2017.01.009 |
| spellingShingle | C. difficile; PaLoc; Binary toxin; CdtR; Virulence Bilverstone, T.W. Kinsmore, N.L. Minton, Nigel P. Kuehne, Sarah A. Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels |
| title | Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels |
| title_full | Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels |
| title_fullStr | Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels |
| title_full_unstemmed | Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels |
| title_short | Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels |
| title_sort | development of clostridium difficile r20291δpaloc model strains and in vitro methodologies reveals cdtr is required for the production of cdt to cytotoxic levels |
| topic | C. difficile; PaLoc; Binary toxin; CdtR; Virulence |
| url | https://eprints.nottingham.ac.uk/39999/ https://eprints.nottingham.ac.uk/39999/ https://eprints.nottingham.ac.uk/39999/ |