Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression

Haplotypes underlying local adaptation and speciation are predicted to have numerous phenotypic effects, but few genes involved have been identified, with much work to date concentrating on visible, morphological, phenotypes. The link between genes controlling these adaptive morphological phenotypes...

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Main Authors: Robertson, Shaun, Bradley, Janette E., MacColl, Andrew D.C.
Format: Article
Published: Nature Publishing Group 2017
Online Access:https://eprints.nottingham.ac.uk/39904/
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author Robertson, Shaun
Bradley, Janette E.
MacColl, Andrew D.C.
author_facet Robertson, Shaun
Bradley, Janette E.
MacColl, Andrew D.C.
author_sort Robertson, Shaun
building Nottingham Research Data Repository
collection Online Access
description Haplotypes underlying local adaptation and speciation are predicted to have numerous phenotypic effects, but few genes involved have been identified, with much work to date concentrating on visible, morphological, phenotypes. The link between genes controlling these adaptive morphological phenotypes and the immune system has seldom been investigated, even though changes in the immune system could have profound adaptive consequences. The Eda gene in three-spined stickleback is one of the best studied major adaptation genes; it directly controls bony plate architecture and has been associated with additional aspects of adaptation to freshwater. Here, we exposed F2 hybrids, used to separate Eda genotype from genetic background, to contrasting conditions in semi-natural enclosures. We demonstrate an association between the Eda haplotype block and the expression pattern of key immune system genes. Furthermore, low plated fish grew less and experienced higher burdens of a common ectoparasite with fitness consequences. Little is currently known about the role of the immune system in facilitating adaptation to novel environments, but this study provides an indication of its potential importance.
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spelling nottingham-399042020-05-04T18:34:50Z https://eprints.nottingham.ac.uk/39904/ Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression Robertson, Shaun Bradley, Janette E. MacColl, Andrew D.C. Haplotypes underlying local adaptation and speciation are predicted to have numerous phenotypic effects, but few genes involved have been identified, with much work to date concentrating on visible, morphological, phenotypes. The link between genes controlling these adaptive morphological phenotypes and the immune system has seldom been investigated, even though changes in the immune system could have profound adaptive consequences. The Eda gene in three-spined stickleback is one of the best studied major adaptation genes; it directly controls bony plate architecture and has been associated with additional aspects of adaptation to freshwater. Here, we exposed F2 hybrids, used to separate Eda genotype from genetic background, to contrasting conditions in semi-natural enclosures. We demonstrate an association between the Eda haplotype block and the expression pattern of key immune system genes. Furthermore, low plated fish grew less and experienced higher burdens of a common ectoparasite with fitness consequences. Little is currently known about the role of the immune system in facilitating adaptation to novel environments, but this study provides an indication of its potential importance. Nature Publishing Group 2017-02-14 Article PeerReviewed Robertson, Shaun, Bradley, Janette E. and MacColl, Andrew D.C. (2017) Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression. Scientific Reports, 7 . p. 42677. ISSN 2045-2322 http://www.nature.com/articles/srep42677 doi:10.1038/srep42677 doi:10.1038/srep42677
spellingShingle Robertson, Shaun
Bradley, Janette E.
MacColl, Andrew D.C.
Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression
title Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression
title_full Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression
title_fullStr Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression
title_full_unstemmed Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression
title_short Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression
title_sort eda haplotypes in three-spined stickleback are associated with variation in immune gene expression
url https://eprints.nottingham.ac.uk/39904/
https://eprints.nottingham.ac.uk/39904/
https://eprints.nottingham.ac.uk/39904/