High resolution 3D imaging of living cells with sub-optical wavelength phonons
Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy. Biologically relevant structural information remains below the Rayleigh limit and beyond the reach of conventional microscopes. Super-resolution techniques are typically based on the...
| Main Authors: | , , , , , |
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| Format: | Article |
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Nature Publishing Group
2016
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| Online Access: | https://eprints.nottingham.ac.uk/39838/ |
| _version_ | 1848795924986331136 |
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| author | Perez-Cota, Fernando Smith, Richard J. Moradi, Emilia Marques, Leonel Webb, Kevin F. Clark, Matt |
| author_facet | Perez-Cota, Fernando Smith, Richard J. Moradi, Emilia Marques, Leonel Webb, Kevin F. Clark, Matt |
| author_sort | Perez-Cota, Fernando |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy. Biologically relevant structural information remains below the Rayleigh limit and beyond the reach of conventional microscopes. Super-resolution techniques are typically based on the nonlinear and stochastic response of fluorescent labels which can be toxic and interfere with cell function. In this paper we present, for the first time, imaging of live cells using sub-optical wavelength phonons. The axial imaging resolution of our system is determined by the acoustic wavelength (λa = λprobe/2n) and not on the NA of the optics allowing sub-optical wavelength acoustic sectioning of samples using the time of flight. The transverse resolution is currently limited to the optical spot size. The contrast mechanism is significantly determined by the mechanical properties of the cells and requires no additional contrast agent, stain or label to image the cell structure. The ability to breach the optical diffraction limit to image living cells acoustically promises to bring a new suite of imaging technologies to bear in answering exigent questions in cell biology and biomedicine. |
| first_indexed | 2025-11-14T19:39:49Z |
| format | Article |
| id | nottingham-39838 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:39:49Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-398382020-05-04T18:25:04Z https://eprints.nottingham.ac.uk/39838/ High resolution 3D imaging of living cells with sub-optical wavelength phonons Perez-Cota, Fernando Smith, Richard J. Moradi, Emilia Marques, Leonel Webb, Kevin F. Clark, Matt Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy. Biologically relevant structural information remains below the Rayleigh limit and beyond the reach of conventional microscopes. Super-resolution techniques are typically based on the nonlinear and stochastic response of fluorescent labels which can be toxic and interfere with cell function. In this paper we present, for the first time, imaging of live cells using sub-optical wavelength phonons. The axial imaging resolution of our system is determined by the acoustic wavelength (λa = λprobe/2n) and not on the NA of the optics allowing sub-optical wavelength acoustic sectioning of samples using the time of flight. The transverse resolution is currently limited to the optical spot size. The contrast mechanism is significantly determined by the mechanical properties of the cells and requires no additional contrast agent, stain or label to image the cell structure. The ability to breach the optical diffraction limit to image living cells acoustically promises to bring a new suite of imaging technologies to bear in answering exigent questions in cell biology and biomedicine. Nature Publishing Group 2016-12-20 Article PeerReviewed Perez-Cota, Fernando, Smith, Richard J., Moradi, Emilia, Marques, Leonel, Webb, Kevin F. and Clark, Matt (2016) High resolution 3D imaging of living cells with sub-optical wavelength phonons. Scientific Reports, 6 . 39326/1-39326/11. ISSN 2045-2322 http://dx.doi.org/10.1038/srep39326 doi:10.1038/srep39326 doi:10.1038/srep39326 |
| spellingShingle | Perez-Cota, Fernando Smith, Richard J. Moradi, Emilia Marques, Leonel Webb, Kevin F. Clark, Matt High resolution 3D imaging of living cells with sub-optical wavelength phonons |
| title | High resolution 3D imaging of living cells with sub-optical wavelength phonons |
| title_full | High resolution 3D imaging of living cells with sub-optical wavelength phonons |
| title_fullStr | High resolution 3D imaging of living cells with sub-optical wavelength phonons |
| title_full_unstemmed | High resolution 3D imaging of living cells with sub-optical wavelength phonons |
| title_short | High resolution 3D imaging of living cells with sub-optical wavelength phonons |
| title_sort | high resolution 3d imaging of living cells with sub-optical wavelength phonons |
| url | https://eprints.nottingham.ac.uk/39838/ https://eprints.nottingham.ac.uk/39838/ https://eprints.nottingham.ac.uk/39838/ |