Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules
Flow cytometry was used to identify mAbs that recognize conserved epitopes on hamster leukocyte differentiation molecules (hLDM) and also to characterize mAbs developed against hLDM. Initial screening of mAbs developed against LDMs in other species yielded mAbs specific for the major histocompatibil...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
Elsevier
2016
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/39753/ |
| _version_ | 1848795905412562944 |
|---|---|
| author | Rees, Jennifer Haig, David Mack, Victoria Davis, William C. |
| author_facet | Rees, Jennifer Haig, David Mack, Victoria Davis, William C. |
| author_sort | Rees, Jennifer |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Flow cytometry was used to identify mAbs that recognize conserved epitopes on hamster leukocyte differentiation molecules (hLDM) and also to characterize mAbs developed against hLDM. Initial screening of mAbs developed against LDMs in other species yielded mAbs specific for the major histocompatibility (MHC) II molecule, CD4 and CD18. Screening of sets of mAbs developed against hLDM yielded 22 new mAbs, including additional mAbs to MHC II molecules and mAbs that recognize LDMs expressed on all leukocytes, granulocytes, all lymphocytes, all T cells, a subset of T cells, or on all B cells. Based on comparison of the pattern of expression of LDMs expressed on all hamster leukocytes with the patterns of expression of known LDMs in other species, as detected by flow cytometry (FC), four mAbs are predicted to recognize CD11a, CD44, and CD45. Cross comparison of mAbs specific for a subset of hamster T cells with a cross reactive mAb known to recognize CD4 in mice and one recognising CD8 revealed they recognize CD4. The characterization of these mAbs expands opportunities to use hamsters as an additional model species to investigate the mechanisms of immunopathogenesis of infectious diseases. |
| first_indexed | 2025-11-14T19:39:31Z |
| format | Article |
| id | nottingham-39753 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:39:31Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-397532020-05-04T18:27:27Z https://eprints.nottingham.ac.uk/39753/ Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules Rees, Jennifer Haig, David Mack, Victoria Davis, William C. Flow cytometry was used to identify mAbs that recognize conserved epitopes on hamster leukocyte differentiation molecules (hLDM) and also to characterize mAbs developed against hLDM. Initial screening of mAbs developed against LDMs in other species yielded mAbs specific for the major histocompatibility (MHC) II molecule, CD4 and CD18. Screening of sets of mAbs developed against hLDM yielded 22 new mAbs, including additional mAbs to MHC II molecules and mAbs that recognize LDMs expressed on all leukocytes, granulocytes, all lymphocytes, all T cells, a subset of T cells, or on all B cells. Based on comparison of the pattern of expression of LDMs expressed on all hamster leukocytes with the patterns of expression of known LDMs in other species, as detected by flow cytometry (FC), four mAbs are predicted to recognize CD11a, CD44, and CD45. Cross comparison of mAbs specific for a subset of hamster T cells with a cross reactive mAb known to recognize CD4 in mice and one recognising CD8 revealed they recognize CD4. The characterization of these mAbs expands opportunities to use hamsters as an additional model species to investigate the mechanisms of immunopathogenesis of infectious diseases. Elsevier 2016-12-06 Article PeerReviewed Rees, Jennifer, Haig, David, Mack, Victoria and Davis, William C. (2016) Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules. Veterinary Immunology and Immunopathology, 183 . pp. 40-44. ISSN 1873-2534 Hamster Monoclonal antibody Flow cytometry CD4 CD8 http://www.sciencedirect.com/science/article/pii/S0165242716303464 doi:10.1016/j.vetimm.2016.12.003 doi:10.1016/j.vetimm.2016.12.003 |
| spellingShingle | Hamster Monoclonal antibody Flow cytometry CD4 CD8 Rees, Jennifer Haig, David Mack, Victoria Davis, William C. Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules |
| title | Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules |
| title_full | Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules |
| title_fullStr | Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules |
| title_full_unstemmed | Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules |
| title_short | Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules |
| title_sort | characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules |
| topic | Hamster Monoclonal antibody Flow cytometry CD4 CD8 |
| url | https://eprints.nottingham.ac.uk/39753/ https://eprints.nottingham.ac.uk/39753/ https://eprints.nottingham.ac.uk/39753/ |