Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography

QR Code

Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography

Raman micro-spectroscopy (RMS) is a non-invasive technique for imaging live cells in-vitro. However, obtaining quantitative molecular information from the Raman spectra is difficult because the intensity of a Raman band is proportional to the number of molecules in the sampled volume, which depends...

Full description

Bibliographic Details
Main Authors:	Boitor, Radu, Sinjab, Faris, Strohbuecker, Stephanie, Sottile, Virginie, Notingher, Ioan
Format:	Article
Published:	Royal Society of Chemistry 2016
Online Access:	https://eprints.nottingham.ac.uk/39602/

Similar Items

Integrated AFM-Raman for molecular characterization of peptide nano- and micro-tubes
by: Sinjab, Faris
Published: (2015)

Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation.
by: Ghita, Adrian, et al.
Published: (2015)

Cytoplasmic RNA in undifferentiated neural stem cells: a potential label-free Raman spectral marker for assessing the undifferentiated status
by: Ghita, Adrian, et al.
Published: (2012)

Label-free Raman hyperspectral imaging of single cells cultured on polymer substrates
by: Sinjab, Faris, et al.
Published: (2017)

Raman spectroscopy: techniques and applications in the life sciences
by: Shipp, Dustin, et al.
Published: (2017)

Applications of spatial light modulators in Raman spectroscopy
by: Sinjab, Faris, et al.
Published: (2019)

Multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery
by: Boitor, Radu
Published: (2019)

Monitoring the mineralisation of bone nodules in vitro by space- and time-resolved Raman micro-spectroscopy.
by: Ghita, Adrian, et al.
Published: (2013)

Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells
by: Sinjab, Faris, et al.
Published: (2018)

Optical sectioning in multi-foci Raman hyperspectral imaging
by: Liao, Zhiyu, et al.
Published: (2018)

Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging
by: Naemat, Abida, et al.
Published: (2018)

Co-localised Raman and force spectroscopy reveal the roles of hydrogen bonds and π-π interactions in defining the mechanical properties of diphenylalanine nano- and micro-tubes
by: Sinjab, Faris, et al.
Published: (2014)

The application of in situ AFM to the study of molecular and macromolecular crystallization
by: Keel, Trevor
Published: (2004)

Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy
by: Ghita, Adrian
Published: (2014)

Polymers with hydro-responsive topography identiﬁed using high throughput AFM of an acrylate microarray
by: Hook, Andrew L., et al.
Published: (2011)

Expression of a SOX1 overlapping transcript in neural differentiation and cancer models
by: Azaz, Ahmad, et al.
Published: (2017)

Diagnosis of tumors during tissue-conserving surgery with integrated autofluorescence and Raman scattering microscopy
by: Kong, Kenny, et al.
Published: (2013)

DMD-based software-configurable spatially-offset Raman spectroscopy for spectral depth-profiling of optically turbid samples
by: Liao, Zhiyu, et al.
Published: (2016)

Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin
by: Rashidi, Hassan, et al.
Published: (2011)

Live quantitative monitoring of mineral deposition in stem cells using tetracycline hydrochloride
by: Macri-Pellizzeri, Laura, et al.
Published: (2018)

Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging
by: Sinjab, Faris, et al.
Published: (2016)

Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy
by: Naemat, Abida, et al.
Published: (2014)

In situ atomic force microscopy (AFM) investigation of kaolinite dissolution in highly caustic environments
by: Chaliha, D., et al.
Published: (2022)

Automated multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery
by: Boitor, Radu, et al.
Published: (2017)

Cell imaging by phonon microscopy: sub-optical wavelength ultrasound for non-invasive imaging
by: Perez-Cota, Fernando, et al.
Published: (2017)

Surface-enhanced Raman spectroscopy study of 4-ATP on gold nanoparticles for basal cell carcinoma fingerprint detection
by: Quynh, Luu Manh, et al.
Published: (2016)

Confocal Raman imaging of live cells
by: Zoladek, Alina
Published: (2011)

Molecular and Genetic Characterisation of Bacterial Cell-Cell Signalling
by: Bastholm, Tahlia
Published: (2023)

Comparative Study of Constant Height Mode and Constant Detection Mode in Atomic Force Microscopy (AFM) Imaging.
by: Lau, Sai Yee
Published: (2016)

In-situ fabrication of gold nanoparticle functionalized probes for tip-enhanced Raman spectroscopy by dielectrophoresis
by: Sweetenham, Claire Sue, et al.
Published: (2016)

Calcium oxalate crystal growth modification; investigations with confocal Raman microscopy
by: McMulkin, Calum J., et al.
Published: (2016)

Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging
by: Naemat, Abida, et al.
Published: (2016)

Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection
by: Corden, Christopher, et al.
Published: (2018)

Feasibility of spatially-offset Raman spectroscopy for in-vitro and in-vivo monitoring mineralisation of bone tissue-engineering scaffolds
by: Liao, Zhiyu, et al.
Published: (2017)

Exposure to the ROCK inhibitor fasudil promotes gliogenesis of neural stem cells in vitro
by: Nizamudeen, Zubair Ahmed, et al.
Published: (2018)

Exposure to the ROCK inhibitor fasudil promotes gliogenesis of neural stem cells in vitro
by: Nizamudeen, Zubair Ahmed, et al.
Published: (2018)

Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings
by: Manzano, Ana Isabel, et al.
Published: (2013)

Molecular quantitation and characterization of Vibrio cholerae from different seafood obtained from wetmarket and supermarket
by: Letchumanan, Vengadesh, et al.
Published: (2012)

Biostatistical analysis of quantitative immunofluorescence microscopy images
by: Giles, C., et al.
Published: (2016)

Effect Of Tetramethylammonium Hydroxide (TMAH) Etchant On The Formation Of Silicon Nanowires Transistor Patterned By Atomic Force Microscopy (AFM) Lithography
by: Lew, Kam Chung
Published: (2011)