A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer's disease risk and increased TREML1 and TREM2 brain gene expression

Introduction: We hypothesized that common Alzheimer's disease (AD)-associated variants within the triggering receptor expressed on myeloid (TREM) gene cluster influence disease through gene expression. Methods: Expression microarrays on temporal cortex and cerebellum from ∼400 neuropathologi...

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Main Authors: Carrasquillo, Minerva, Allen, Mariet, Burgess, Jeremy D., Medway, Christopher, Morgan, Kevin
Format: Article
Published: Elsevier 2017
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Online Access:https://eprints.nottingham.ac.uk/39400/
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author Carrasquillo, Minerva
Allen, Mariet
Burgess, Jeremy D.
Medway, Christopher
Morgan, Kevin
author_facet Carrasquillo, Minerva
Allen, Mariet
Burgess, Jeremy D.
Medway, Christopher
Morgan, Kevin
author_sort Carrasquillo, Minerva
building Nottingham Research Data Repository
collection Online Access
description Introduction: We hypothesized that common Alzheimer's disease (AD)-associated variants within the triggering receptor expressed on myeloid (TREM) gene cluster influence disease through gene expression. Methods: Expression microarrays on temporal cortex and cerebellum from ∼400 neuropathologically diagnosed subjects and two independent RNAseq replication cohorts were used for expression quantitative trait locus analysis. Results: A variant within a DNase hypersensitive site 5′ of TREM2, rs9357347-C, associates with reduced AD risk and increased TREML1 and TREM2 levels (uncorrected P = 6.3 × 10−3 and 4.6 × 10−2, respectively). Meta-analysis on expression quantitative trait locus results from three independent data sets (n = 1006) confirmed these associations (uncorrected P = 3.4 × 10−2 and 3.5 × 10−3, Bonferroni-corrected P = 6.7 × 10−2 and 7.1 × 10−3, respectively). Discussion: Our findings point to rs9357347 as a functional regulatory variant that contributes to a protective effect observed at the TREM locus in the International Genomics of Alzheimer's Project genome-wide association study meta-analysis and suggest concomitant increase in TREML1 and TREM2 brain levels as a potential mechanism for protection from AD.
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spelling nottingham-394002020-05-04T19:56:53Z https://eprints.nottingham.ac.uk/39400/ A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer's disease risk and increased TREML1 and TREM2 brain gene expression Carrasquillo, Minerva Allen, Mariet Burgess, Jeremy D. Medway, Christopher Morgan, Kevin Introduction: We hypothesized that common Alzheimer's disease (AD)-associated variants within the triggering receptor expressed on myeloid (TREM) gene cluster influence disease through gene expression. Methods: Expression microarrays on temporal cortex and cerebellum from ∼400 neuropathologically diagnosed subjects and two independent RNAseq replication cohorts were used for expression quantitative trait locus analysis. Results: A variant within a DNase hypersensitive site 5′ of TREM2, rs9357347-C, associates with reduced AD risk and increased TREML1 and TREM2 levels (uncorrected P = 6.3 × 10−3 and 4.6 × 10−2, respectively). Meta-analysis on expression quantitative trait locus results from three independent data sets (n = 1006) confirmed these associations (uncorrected P = 3.4 × 10−2 and 3.5 × 10−3, Bonferroni-corrected P = 6.7 × 10−2 and 7.1 × 10−3, respectively). Discussion: Our findings point to rs9357347 as a functional regulatory variant that contributes to a protective effect observed at the TREM locus in the International Genomics of Alzheimer's Project genome-wide association study meta-analysis and suggest concomitant increase in TREML1 and TREM2 brain levels as a potential mechanism for protection from AD. Elsevier 2017-06 Article PeerReviewed Carrasquillo, Minerva, Allen, Mariet, Burgess, Jeremy D., Medway, Christopher and Morgan, Kevin (2017) A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer's disease risk and increased TREML1 and TREM2 brain gene expression. Alzheimer's & Dementia, 13 (6). pp. 663-673. ISSN 1552-5260 Alzheimer's disease; eQTL; TREM2; TREML1; Regulatory variant http://www.sciencedirect.com/science/article/pii/S1552526016330710 doi:10.1016/j.jalz.2016.10.005 doi:10.1016/j.jalz.2016.10.005
spellingShingle Alzheimer's disease; eQTL; TREM2; TREML1; Regulatory variant
Carrasquillo, Minerva
Allen, Mariet
Burgess, Jeremy D.
Medway, Christopher
Morgan, Kevin
A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer's disease risk and increased TREML1 and TREM2 brain gene expression
title A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer's disease risk and increased TREML1 and TREM2 brain gene expression
title_full A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer's disease risk and increased TREML1 and TREM2 brain gene expression
title_fullStr A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer's disease risk and increased TREML1 and TREM2 brain gene expression
title_full_unstemmed A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer's disease risk and increased TREML1 and TREM2 brain gene expression
title_short A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer's disease risk and increased TREML1 and TREM2 brain gene expression
title_sort candidate regulatory variant at the trem gene cluster associates with decreased alzheimer's disease risk and increased treml1 and trem2 brain gene expression
topic Alzheimer's disease; eQTL; TREM2; TREML1; Regulatory variant
url https://eprints.nottingham.ac.uk/39400/
https://eprints.nottingham.ac.uk/39400/
https://eprints.nottingham.ac.uk/39400/