Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides
The aim of this work was to develop drug-soft contact lens combination products suitable for controlled release of antimicrobial peptides on the ocular surface. Incorporation of functional monomers and the application of molecular imprinting techniques were explored to endow 2-hydroxyethyl methacryl...
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| Format: | Article |
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Elsevier
2015
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| Online Access: | https://eprints.nottingham.ac.uk/39083/ |
| _version_ | 1848795759646867456 |
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| author | Malakooti, Negin Alexander, Cameron Alvarez-Lorenzo, Carmen |
| author_facet | Malakooti, Negin Alexander, Cameron Alvarez-Lorenzo, Carmen |
| author_sort | Malakooti, Negin |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The aim of this work was to develop drug-soft contact lens combination products suitable for controlled release of antimicrobial peptides on the ocular surface. Incorporation of functional monomers and the application of molecular imprinting techniques were explored to endow 2-hydroxyethyl methacrylate (HEMA) hydrogels with the ability to load and to sustain the release of polymyxin B and vancomycin. Various HEMA–drug–functional monomer–cross–linker molar ratios were evaluated to prepare polymyxin B imprinted and non-imprinted hydrogels. Acrylic acid-functionalized and imprinted hydrogels loaded greater amounts of polymyxin B and led to more sustained release profiles, in comparison with non-functionalized and non-imprinted networks. Polymyxin B-loaded hydrogels showed good biocompatibility in hen’s egg test-chorioallantoic membrane tests. Functionalized hydrogels also loaded vancomycin and sustained its release, but the imprinting effect was only exhibited with polymyxin B, as demonstrated in rebinding tests. Microbiological assays carried out with Pseudomonas aeruginosa allowed identification of the most suitable hydrogel composition for efficient bacteria eradication; some hydrogels being able to stand several continued challenges against this important bacterial pathogen. |
| first_indexed | 2025-11-14T19:37:12Z |
| format | Article |
| id | nottingham-39083 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:37:12Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-390832020-05-04T17:18:22Z https://eprints.nottingham.ac.uk/39083/ Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides Malakooti, Negin Alexander, Cameron Alvarez-Lorenzo, Carmen The aim of this work was to develop drug-soft contact lens combination products suitable for controlled release of antimicrobial peptides on the ocular surface. Incorporation of functional monomers and the application of molecular imprinting techniques were explored to endow 2-hydroxyethyl methacrylate (HEMA) hydrogels with the ability to load and to sustain the release of polymyxin B and vancomycin. Various HEMA–drug–functional monomer–cross–linker molar ratios were evaluated to prepare polymyxin B imprinted and non-imprinted hydrogels. Acrylic acid-functionalized and imprinted hydrogels loaded greater amounts of polymyxin B and led to more sustained release profiles, in comparison with non-functionalized and non-imprinted networks. Polymyxin B-loaded hydrogels showed good biocompatibility in hen’s egg test-chorioallantoic membrane tests. Functionalized hydrogels also loaded vancomycin and sustained its release, but the imprinting effect was only exhibited with polymyxin B, as demonstrated in rebinding tests. Microbiological assays carried out with Pseudomonas aeruginosa allowed identification of the most suitable hydrogel composition for efficient bacteria eradication; some hydrogels being able to stand several continued challenges against this important bacterial pathogen. Elsevier 2015-10-31 Article PeerReviewed Malakooti, Negin, Alexander, Cameron and Alvarez-Lorenzo, Carmen (2015) Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides. Journal of Pharmaceutical Sciences, 104 (10). pp. 3386-3394. ISSN 1520-6017 Hydrogels; Biomaterials; Controlled release; Peptide delivery; Mucosal delivery; Drug-device combination product; Molecular imprinting; Ophthalmic drug delivery; Contact lens; Pseudomonas aeruginosa http://dx.doi.org/10.1002/jps.24537 doi:10.1002/jps.24537 doi:10.1002/jps.24537 |
| spellingShingle | Hydrogels; Biomaterials; Controlled release; Peptide delivery; Mucosal delivery; Drug-device combination product; Molecular imprinting; Ophthalmic drug delivery; Contact lens; Pseudomonas aeruginosa Malakooti, Negin Alexander, Cameron Alvarez-Lorenzo, Carmen Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides |
| title | Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides |
| title_full | Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides |
| title_fullStr | Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides |
| title_full_unstemmed | Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides |
| title_short | Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides |
| title_sort | imprinted contact lenses for sustained release of polymyxin b and related antimicrobial peptides |
| topic | Hydrogels; Biomaterials; Controlled release; Peptide delivery; Mucosal delivery; Drug-device combination product; Molecular imprinting; Ophthalmic drug delivery; Contact lens; Pseudomonas aeruginosa |
| url | https://eprints.nottingham.ac.uk/39083/ https://eprints.nottingham.ac.uk/39083/ https://eprints.nottingham.ac.uk/39083/ |