Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state
Seed dormancy is a genetically controlled block preventing the germination of imbibed seeds in favorable conditions. It requires a period of dry storage (after-ripening) or certain environmental conditions to be overcome. Dormancy is an important seed trait, which is under selective pressure, to con...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Frontiers Media
2016
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/38963/ |
| _version_ | 1848795729414324224 |
|---|---|
| author | Dekkers, Bas J.W. Pearce, Simon P. van Bolderen-Veldkamp, R.P.M. Holdsworth, Michael J. Bentsink, Leónie |
| author_facet | Dekkers, Bas J.W. Pearce, Simon P. van Bolderen-Veldkamp, R.P.M. Holdsworth, Michael J. Bentsink, Leónie |
| author_sort | Dekkers, Bas J.W. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Seed dormancy is a genetically controlled block preventing the germination of imbibed seeds in favorable conditions. It requires a period of dry storage (after-ripening) or certain environmental conditions to be overcome. Dormancy is an important seed trait, which is under selective pressure, to control the seasonal timing of seed germination. Dormant and non-dormant (after-ripened) seeds are characterized by large sets of differentially expressed genes. However, little information is available concerning the temporal and spatial transcriptional changes during early stages of rehydration in dormant and nondormant seeds. We employed genome-wide transcriptome analysis on seeds of the model plant Arabidopsis thaliana to investigate transcriptional changes in dry seeds upon rehydration. We analyzed gene expression of dormant and after-ripened seeds of the Cvi accession over four time points and two seed compartments (the embryo and surrounding single cell layer endosperm), during the first 24 h after sowing. This work provides a global view of gene expression changes in dormant and non-dormant seeds with temporal and spatial detail, and these may be visualized via a web accessible tool (http://www.wageningenseedlab.nl/resources). A large proportion of transcripts change similarly in both dormant and non-dormant seeds upon rehydration, however, the first differences in transcript abundances become visible shortly after the initiation of imbibition, indicating that changes induced by after-ripening are detected and responded to rapidly upon rehydration. We identified several gene expression profiles which contribute to differential gene expression between dormant and non-dormant samples. Genes with enhanced expression in the endosperm of dormant seeds were overrepresented for stress-related Gene Ontology categories, suggesting a protective role for the endosperm against biotic and abiotic stress to support persistence of the dormant seed in its environment. |
| first_indexed | 2025-11-14T19:36:43Z |
| format | Article |
| id | nottingham-38963 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:36:43Z |
| publishDate | 2016 |
| publisher | Frontiers Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-389632020-05-04T18:05:18Z https://eprints.nottingham.ac.uk/38963/ Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state Dekkers, Bas J.W. Pearce, Simon P. van Bolderen-Veldkamp, R.P.M. Holdsworth, Michael J. Bentsink, Leónie Seed dormancy is a genetically controlled block preventing the germination of imbibed seeds in favorable conditions. It requires a period of dry storage (after-ripening) or certain environmental conditions to be overcome. Dormancy is an important seed trait, which is under selective pressure, to control the seasonal timing of seed germination. Dormant and non-dormant (after-ripened) seeds are characterized by large sets of differentially expressed genes. However, little information is available concerning the temporal and spatial transcriptional changes during early stages of rehydration in dormant and nondormant seeds. We employed genome-wide transcriptome analysis on seeds of the model plant Arabidopsis thaliana to investigate transcriptional changes in dry seeds upon rehydration. We analyzed gene expression of dormant and after-ripened seeds of the Cvi accession over four time points and two seed compartments (the embryo and surrounding single cell layer endosperm), during the first 24 h after sowing. This work provides a global view of gene expression changes in dormant and non-dormant seeds with temporal and spatial detail, and these may be visualized via a web accessible tool (http://www.wageningenseedlab.nl/resources). A large proportion of transcripts change similarly in both dormant and non-dormant seeds upon rehydration, however, the first differences in transcript abundances become visible shortly after the initiation of imbibition, indicating that changes induced by after-ripening are detected and responded to rapidly upon rehydration. We identified several gene expression profiles which contribute to differential gene expression between dormant and non-dormant samples. Genes with enhanced expression in the endosperm of dormant seeds were overrepresented for stress-related Gene Ontology categories, suggesting a protective role for the endosperm against biotic and abiotic stress to support persistence of the dormant seed in its environment. Frontiers Media 2016-08-30 Article PeerReviewed Dekkers, Bas J.W., Pearce, Simon P., van Bolderen-Veldkamp, R.P.M., Holdsworth, Michael J. and Bentsink, Leónie (2016) Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state. Frontiers in Plant Science, 7 . 1323/1-1323/15. ISSN 1664-462X After-ripening Arabidopsis Dormancy Embryo Endosperm Seed Transcriptome http://dx.doi.org/10.3389/fpls.2016.01323 doi:10.3389/fpls.2016.01323 doi:10.3389/fpls.2016.01323 |
| spellingShingle | After-ripening Arabidopsis Dormancy Embryo Endosperm Seed Transcriptome Dekkers, Bas J.W. Pearce, Simon P. van Bolderen-Veldkamp, R.P.M. Holdsworth, Michael J. Bentsink, Leónie Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state |
| title | Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state |
| title_full | Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state |
| title_fullStr | Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state |
| title_full_unstemmed | Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state |
| title_short | Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state |
| title_sort | dormant and after-ripened arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state |
| topic | After-ripening Arabidopsis Dormancy Embryo Endosperm Seed Transcriptome |
| url | https://eprints.nottingham.ac.uk/38963/ https://eprints.nottingham.ac.uk/38963/ https://eprints.nottingham.ac.uk/38963/ |