The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes
Taste is perceived as one of a combination of five sensations, sweet, sour, bitter, salty, and umami. The umami taste is best known as a savoury sensation and plays a central role in food flavour, palatability, and eating satisfaction. Umami flavour can be imparted by the presence of glutamate and i...
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| Format: | Article |
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Springer
2016
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| Online Access: | https://eprints.nottingham.ac.uk/38959/ |
| _version_ | 1848795728632086528 |
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| author | Chew, Bee Lynn Fisk, Ian D. Fray, Rupert Tucker, Gregory A. Bodi, Zsuzsanna Ferguson, Alison Xiao, Wei Seymour, Graham B. |
| author_facet | Chew, Bee Lynn Fisk, Ian D. Fray, Rupert Tucker, Gregory A. Bodi, Zsuzsanna Ferguson, Alison Xiao, Wei Seymour, Graham B. |
| author_sort | Chew, Bee Lynn |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Taste is perceived as one of a combination of five sensations, sweet, sour, bitter, salty, and umami. The umami taste is best known as a savoury sensation and plays a central role in food flavour, palatability, and eating satisfaction. Umami flavour can be imparted by the presence of glutamate and is greatly enhanced by the addition of ribonucleotides, such as inosine monophosphate (IMP) and guanosine monophosphate (GMP). The production of IMP is regulated by the enzyme adenosine monophosphate (AMP) deaminase which functions to convert AMP into IMP. We have generated transgenic tomato (Solanum lycopersicum) lines over expressing AMP deaminase under the control of a fruit-specific promoter. The transgenic lines showed substantially enhanced levels of AMP deaminase expression in comparison to the wild-type control. Elevated AMP deaminase levels resulted in the reduced accumulation of glutamate and increased levels of the umami nucleotide GMP. AMP concentrations were unchanged. The effects on the levels of glutamate and GMP were unexpected and are discussed in relation to the metabolite flux within this pathway. |
| first_indexed | 2025-11-14T19:36:42Z |
| format | Article |
| id | nottingham-38959 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:36:42Z |
| publishDate | 2016 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-389592020-05-04T18:09:53Z https://eprints.nottingham.ac.uk/38959/ The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes Chew, Bee Lynn Fisk, Ian D. Fray, Rupert Tucker, Gregory A. Bodi, Zsuzsanna Ferguson, Alison Xiao, Wei Seymour, Graham B. Taste is perceived as one of a combination of five sensations, sweet, sour, bitter, salty, and umami. The umami taste is best known as a savoury sensation and plays a central role in food flavour, palatability, and eating satisfaction. Umami flavour can be imparted by the presence of glutamate and is greatly enhanced by the addition of ribonucleotides, such as inosine monophosphate (IMP) and guanosine monophosphate (GMP). The production of IMP is regulated by the enzyme adenosine monophosphate (AMP) deaminase which functions to convert AMP into IMP. We have generated transgenic tomato (Solanum lycopersicum) lines over expressing AMP deaminase under the control of a fruit-specific promoter. The transgenic lines showed substantially enhanced levels of AMP deaminase expression in comparison to the wild-type control. Elevated AMP deaminase levels resulted in the reduced accumulation of glutamate and increased levels of the umami nucleotide GMP. AMP concentrations were unchanged. The effects on the levels of glutamate and GMP were unexpected and are discussed in relation to the metabolite flux within this pathway. Springer 2016-09-23 Article PeerReviewed Chew, Bee Lynn, Fisk, Ian D., Fray, Rupert, Tucker, Gregory A., Bodi, Zsuzsanna, Ferguson, Alison, Xiao, Wei and Seymour, Graham B. (2016) The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes. Plant Cell Reports, 36 (1). pp. 81-87. ISSN 1432-203X umami transgenic tomato glumate adenosine monophosphate guanosine monophosphate https://link.springer.com/article/10.1007%2Fs00299-016-2058-z doi:10.1007/s00299-016-2058-z doi:10.1007/s00299-016-2058-z |
| spellingShingle | umami transgenic tomato glumate adenosine monophosphate guanosine monophosphate Chew, Bee Lynn Fisk, Ian D. Fray, Rupert Tucker, Gregory A. Bodi, Zsuzsanna Ferguson, Alison Xiao, Wei Seymour, Graham B. The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes |
| title | The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes |
| title_full | The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes |
| title_fullStr | The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes |
| title_full_unstemmed | The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes |
| title_short | The effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes |
| title_sort | effect of adenosine monophosphate deaminase overexpression on the accumulation of umami-related metabolites in tomatoes |
| topic | umami transgenic tomato glumate adenosine monophosphate guanosine monophosphate |
| url | https://eprints.nottingham.ac.uk/38959/ https://eprints.nottingham.ac.uk/38959/ https://eprints.nottingham.ac.uk/38959/ |