Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants

Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes that enable this activity remain largely unknown. Here we show that BRs participate in the regulation of freezing tolerance. BR signaling-defective mutants of Arabidopsis thal...

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Main Authors: Eremina, Marina, Unterholzner, Simon J., Rathnayake, Ajith I., Castellanos, Marcos, Khan, Mamoona, Kugler, Karl G., May, Sean, Mayer, Klaus F.X., Rozhon, Wilfried, Poppenberger, Brigitte
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Published: National Academy of Sciences 2016
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Online Access:https://eprints.nottingham.ac.uk/38945/
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author Eremina, Marina
Unterholzner, Simon J.
Rathnayake, Ajith I.
Castellanos, Marcos
Khan, Mamoona
Kugler, Karl G.
May, Sean
Mayer, Klaus F.X.
Rozhon, Wilfried
Poppenberger, Brigitte
author_facet Eremina, Marina
Unterholzner, Simon J.
Rathnayake, Ajith I.
Castellanos, Marcos
Khan, Mamoona
Kugler, Karl G.
May, Sean
Mayer, Klaus F.X.
Rozhon, Wilfried
Poppenberger, Brigitte
author_sort Eremina, Marina
building Nottingham Research Data Repository
collection Online Access
description Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes that enable this activity remain largely unknown. Here we show that BRs participate in the regulation of freezing tolerance. BR signaling-defective mutants of Arabidopsis thaliana were hypersensitive to freezing before and after cold acclimation. The constitutive activation of BR signaling, in contrast, enhanced freezing resistance. Evidence is provided that the BR-controlled basic helix–loop–helix transcription factor CESTA (CES) can contribute to the constitutive expression of the C-REPEAT/DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR (CBF) transcriptional regulators that control cold responsive (COR) gene expression. In addition, CBF-independent classes of BR-regulated COR genes are identified that are regulated in a BR- and CES-dependent manner during cold acclimation. A model is presented in which BRs govern different cold-responsive transcriptional cascades through the posttranslational modification of CES and redundantly acting factors. This contributes to the basal resistance against freezing stress, but also to the further improvement of this resistance through cold acclimation.
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spelling nottingham-389452020-05-04T18:18:00Z https://eprints.nottingham.ac.uk/38945/ Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants Eremina, Marina Unterholzner, Simon J. Rathnayake, Ajith I. Castellanos, Marcos Khan, Mamoona Kugler, Karl G. May, Sean Mayer, Klaus F.X. Rozhon, Wilfried Poppenberger, Brigitte Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes that enable this activity remain largely unknown. Here we show that BRs participate in the regulation of freezing tolerance. BR signaling-defective mutants of Arabidopsis thaliana were hypersensitive to freezing before and after cold acclimation. The constitutive activation of BR signaling, in contrast, enhanced freezing resistance. Evidence is provided that the BR-controlled basic helix–loop–helix transcription factor CESTA (CES) can contribute to the constitutive expression of the C-REPEAT/DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR (CBF) transcriptional regulators that control cold responsive (COR) gene expression. In addition, CBF-independent classes of BR-regulated COR genes are identified that are regulated in a BR- and CES-dependent manner during cold acclimation. A model is presented in which BRs govern different cold-responsive transcriptional cascades through the posttranslational modification of CES and redundantly acting factors. This contributes to the basal resistance against freezing stress, but also to the further improvement of this resistance through cold acclimation. National Academy of Sciences 2016-10-04 Article PeerReviewed Eremina, Marina, Unterholzner, Simon J., Rathnayake, Ajith I., Castellanos, Marcos, Khan, Mamoona, Kugler, Karl G., May, Sean, Mayer, Klaus F.X., Rozhon, Wilfried and Poppenberger, Brigitte (2016) Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. Proceedings of the National Academy of Sciences, 113 (40). E5982-E5991. ISSN 1091-6490 Brassinosteroid CESTA Cold Responses CBFs CBF1 http://www.pnas.org/content/113/40/E5982 doi:10.1073/pnas.1611477113 doi:10.1073/pnas.1611477113
spellingShingle Brassinosteroid
CESTA
Cold Responses
CBFs
CBF1
Eremina, Marina
Unterholzner, Simon J.
Rathnayake, Ajith I.
Castellanos, Marcos
Khan, Mamoona
Kugler, Karl G.
May, Sean
Mayer, Klaus F.X.
Rozhon, Wilfried
Poppenberger, Brigitte
Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
title Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
title_full Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
title_fullStr Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
title_full_unstemmed Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
title_short Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
title_sort brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
topic Brassinosteroid
CESTA
Cold Responses
CBFs
CBF1
url https://eprints.nottingham.ac.uk/38945/
https://eprints.nottingham.ac.uk/38945/
https://eprints.nottingham.ac.uk/38945/