Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility

Retrospective examination of breeding records enabled the identification of 10 dogs of normal fertility and 10 dogs with established infertility of at least 12 months of duration. Comparisons of testicular palpation, semen evaluation, testicular ultrasound examination, Doppler ultrasound measurement...

Full description

Bibliographic Details
Main Authors: de Souza, Mírley B., England, Gary C.W., Mota Filho, Antônio Cavalcante, Ackermann, Camila Louise, Sousa, Carmen Vládia S., de Carvalho, Gabriela G., Silva, Herlon Victor Redrigues, Pinto, José N., Linhares, Jussiara C.S., Oba, Eunice, da Silva, Lucia Daniel Machado
Format: Article
Published: Elsevier 2015
Subjects:
Online Access:https://eprints.nottingham.ac.uk/38920/
_version_ 1848795720995307520
author de Souza, Mírley B.
England, Gary C.W.
Mota Filho, Antônio Cavalcante
Ackermann, Camila Louise
Sousa, Carmen Vládia S.
de Carvalho, Gabriela G.
Silva, Herlon Victor Redrigues
Pinto, José N.
Linhares, Jussiara C.S.
Oba, Eunice
da Silva, Lucia Daniel Machado
author_facet de Souza, Mírley B.
England, Gary C.W.
Mota Filho, Antônio Cavalcante
Ackermann, Camila Louise
Sousa, Carmen Vládia S.
de Carvalho, Gabriela G.
Silva, Herlon Victor Redrigues
Pinto, José N.
Linhares, Jussiara C.S.
Oba, Eunice
da Silva, Lucia Daniel Machado
author_sort de Souza, Mírley B.
building Nottingham Research Data Repository
collection Online Access
description Retrospective examination of breeding records enabled the identification of 10 dogs of normal fertility and 10 dogs with established infertility of at least 12 months of duration. Comparisons of testicular palpation, semen evaluation, testicular ultrasound examination, Doppler ultrasound measurement of testicular artery blood flow, and measurement of serum testosterone concentration were made between the two groups over weekly examinations performed on three occasions. There were no differences in testicular volume (cm3) between the two groups (fertile right testis = 10.77 ± 1.66; fertile left testis = 12.17 ± 2.22); (infertile right testis = 10.25 ± 3.33; infertile left testis = 11.37 ± 3.30), although the infertile dogs all had subjectively softer testes compared with the fertile dogs. Infertile dogs were either azoospermic or when they ejaculated, they had lower sperm concentration, sperm motility, and percentage of morphologically normal spermatozoa than fertile dogs. Furthermore, infertile dogs had reduced sperm membrane integrity measured via the hypoosmotic swelling test. Infertile dogs had significantly lower basal serum testosterone concentrations (1.40 ± 0.62 ng/mL) than fertile dogs (1.81 ± 0.87 ng/mL; P < 0.05). There were subjective differences in testicular echogenicity in some of the infertile dogs, and important differences in testicular artery blood flow with lower peak systolic and end-diastolic velocities measured in the distal supratesticular artery, marginal testicular artery, and intratesticular artery of infertile dogs (P < 0.05). Notably, resistance index and pulsatility index did not differ between infertile and fertile dogs. These findings report important differences between infertile and fertile dogs which may be detected within an expanded breeding soundness examination.
first_indexed 2025-11-14T19:36:35Z
format Article
id nottingham-38920
institution University of Nottingham Malaysia Campus
institution_category Local University
last_indexed 2025-11-14T19:36:35Z
publishDate 2015
publisher Elsevier
recordtype eprints
repository_type Digital Repository
spelling nottingham-389202020-05-04T17:07:58Z https://eprints.nottingham.ac.uk/38920/ Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility de Souza, Mírley B. England, Gary C.W. Mota Filho, Antônio Cavalcante Ackermann, Camila Louise Sousa, Carmen Vládia S. de Carvalho, Gabriela G. Silva, Herlon Victor Redrigues Pinto, José N. Linhares, Jussiara C.S. Oba, Eunice da Silva, Lucia Daniel Machado Retrospective examination of breeding records enabled the identification of 10 dogs of normal fertility and 10 dogs with established infertility of at least 12 months of duration. Comparisons of testicular palpation, semen evaluation, testicular ultrasound examination, Doppler ultrasound measurement of testicular artery blood flow, and measurement of serum testosterone concentration were made between the two groups over weekly examinations performed on three occasions. There were no differences in testicular volume (cm3) between the two groups (fertile right testis = 10.77 ± 1.66; fertile left testis = 12.17 ± 2.22); (infertile right testis = 10.25 ± 3.33; infertile left testis = 11.37 ± 3.30), although the infertile dogs all had subjectively softer testes compared with the fertile dogs. Infertile dogs were either azoospermic or when they ejaculated, they had lower sperm concentration, sperm motility, and percentage of morphologically normal spermatozoa than fertile dogs. Furthermore, infertile dogs had reduced sperm membrane integrity measured via the hypoosmotic swelling test. Infertile dogs had significantly lower basal serum testosterone concentrations (1.40 ± 0.62 ng/mL) than fertile dogs (1.81 ± 0.87 ng/mL; P < 0.05). There were subjective differences in testicular echogenicity in some of the infertile dogs, and important differences in testicular artery blood flow with lower peak systolic and end-diastolic velocities measured in the distal supratesticular artery, marginal testicular artery, and intratesticular artery of infertile dogs (P < 0.05). Notably, resistance index and pulsatility index did not differ between infertile and fertile dogs. These findings report important differences between infertile and fertile dogs which may be detected within an expanded breeding soundness examination. Elsevier 2015-05-27 Article PeerReviewed de Souza, Mírley B., England, Gary C.W., Mota Filho, Antônio Cavalcante, Ackermann, Camila Louise, Sousa, Carmen Vládia S., de Carvalho, Gabriela G., Silva, Herlon Victor Redrigues, Pinto, José N., Linhares, Jussiara C.S., Oba, Eunice and da Silva, Lucia Daniel Machado (2015) Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility. Theriogenology, 84 (5). pp. 805-810. ISSN 1879-3231 Ultrasonography; Pulse-wave Doppler; Dog; Testis; Infertility http://www.sciencedirect.com/science/article/pii/S0093691X15002794 doi:10.1016/j.theriogenology.2015.05.015 doi:10.1016/j.theriogenology.2015.05.015
spellingShingle Ultrasonography; Pulse-wave Doppler; Dog; Testis; Infertility
de Souza, Mírley B.
England, Gary C.W.
Mota Filho, Antônio Cavalcante
Ackermann, Camila Louise
Sousa, Carmen Vládia S.
de Carvalho, Gabriela G.
Silva, Herlon Victor Redrigues
Pinto, José N.
Linhares, Jussiara C.S.
Oba, Eunice
da Silva, Lucia Daniel Machado
Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility
title Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility
title_full Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility
title_fullStr Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility
title_full_unstemmed Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility
title_short Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility
title_sort semen quality, testicular b-mode and doppler ultrasound, and serum testosterone concentrations in dogs with established infertility
topic Ultrasonography; Pulse-wave Doppler; Dog; Testis; Infertility
url https://eprints.nottingham.ac.uk/38920/
https://eprints.nottingham.ac.uk/38920/
https://eprints.nottingham.ac.uk/38920/