Analysis of root growth from a phenotyping data set using a density-based model

Major research efforts are targeting the improved performance of root systems for more efficient use of water and nutrients by crops. However, characterizing root system architecture (RSA) is challenging, because roots are difficult objects to observe and analyse. A model-based analysis of RSA trait...

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Main Authors: Kalogiros, Dimitris I., Adu, Michael Osei, White, Philip J., Broadley, Martin R., Draye, Xavier, Ptashnyk, Mariya, Bengough, A. Glyn, Dupuy, Lionel X.
Format: Article
Published: Oxford University Press 2016
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Online Access:https://eprints.nottingham.ac.uk/38815/
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author Kalogiros, Dimitris I.
Adu, Michael Osei
White, Philip J.
Broadley, Martin R.
Draye, Xavier
Ptashnyk, Mariya
Bengough, A. Glyn
Dupuy, Lionel X.
author_facet Kalogiros, Dimitris I.
Adu, Michael Osei
White, Philip J.
Broadley, Martin R.
Draye, Xavier
Ptashnyk, Mariya
Bengough, A. Glyn
Dupuy, Lionel X.
author_sort Kalogiros, Dimitris I.
building Nottingham Research Data Repository
collection Online Access
description Major research efforts are targeting the improved performance of root systems for more efficient use of water and nutrients by crops. However, characterizing root system architecture (RSA) is challenging, because roots are difficult objects to observe and analyse. A model-based analysis of RSA traits from phenotyping image data is presented. The model can successfully back-calculate growth parameters without the need to measure individual roots. The mathematical model uses partial differential equations to describe root system development. Methods based on kernel estimators were used to quantify root density distributions from experimental image data, and different optimization approaches to parameterize the model were tested. The model was tested on root images of a set of 89 Brassica rapa L. individuals of the same genotype grown for 14 d after sowing on blue filter paper. Optimized root growth parameters enabled the final (modelled) length of the main root axes to be matched within 1% of their mean values observed in experiments. Parameterized values for elongation rates were within ±4% of the values measured directly on images. Future work should investigate the time dependency of growth parameters using time-lapse image data. The approach is a potentially powerful quantitative technique for identifying crop genotypes with more efficient root systems, using (even incomplete) data from high-throughput phenotyping systems.
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spelling nottingham-388152020-05-04T17:38:03Z https://eprints.nottingham.ac.uk/38815/ Analysis of root growth from a phenotyping data set using a density-based model Kalogiros, Dimitris I. Adu, Michael Osei White, Philip J. Broadley, Martin R. Draye, Xavier Ptashnyk, Mariya Bengough, A. Glyn Dupuy, Lionel X. Major research efforts are targeting the improved performance of root systems for more efficient use of water and nutrients by crops. However, characterizing root system architecture (RSA) is challenging, because roots are difficult objects to observe and analyse. A model-based analysis of RSA traits from phenotyping image data is presented. The model can successfully back-calculate growth parameters without the need to measure individual roots. The mathematical model uses partial differential equations to describe root system development. Methods based on kernel estimators were used to quantify root density distributions from experimental image data, and different optimization approaches to parameterize the model were tested. The model was tested on root images of a set of 89 Brassica rapa L. individuals of the same genotype grown for 14 d after sowing on blue filter paper. Optimized root growth parameters enabled the final (modelled) length of the main root axes to be matched within 1% of their mean values observed in experiments. Parameterized values for elongation rates were within ±4% of the values measured directly on images. Future work should investigate the time dependency of growth parameters using time-lapse image data. The approach is a potentially powerful quantitative technique for identifying crop genotypes with more efficient root systems, using (even incomplete) data from high-throughput phenotyping systems. Oxford University Press 2016-02-04 Article PeerReviewed Kalogiros, Dimitris I., Adu, Michael Osei, White, Philip J., Broadley, Martin R., Draye, Xavier, Ptashnyk, Mariya, Bengough, A. Glyn and Dupuy, Lionel X. (2016) Analysis of root growth from a phenotyping data set using a density-based model. Journal of Experimental Botany, 67 (4). pp. 1045-1058. ISSN 1460-2431 denisty-based models kernel-based non-parametric methods model validation optimization root system architecture time-delay partial differential equations http://jxb.oxfordjournals.org/content/67/4/1045 doi:10.1093/jxb/erv573 doi:10.1093/jxb/erv573
spellingShingle denisty-based models
kernel-based non-parametric methods
model validation
optimization
root system architecture
time-delay partial differential equations
Kalogiros, Dimitris I.
Adu, Michael Osei
White, Philip J.
Broadley, Martin R.
Draye, Xavier
Ptashnyk, Mariya
Bengough, A. Glyn
Dupuy, Lionel X.
Analysis of root growth from a phenotyping data set using a density-based model
title Analysis of root growth from a phenotyping data set using a density-based model
title_full Analysis of root growth from a phenotyping data set using a density-based model
title_fullStr Analysis of root growth from a phenotyping data set using a density-based model
title_full_unstemmed Analysis of root growth from a phenotyping data set using a density-based model
title_short Analysis of root growth from a phenotyping data set using a density-based model
title_sort analysis of root growth from a phenotyping data set using a density-based model
topic denisty-based models
kernel-based non-parametric methods
model validation
optimization
root system architecture
time-delay partial differential equations
url https://eprints.nottingham.ac.uk/38815/
https://eprints.nottingham.ac.uk/38815/
https://eprints.nottingham.ac.uk/38815/