The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection
A screen of bacteriophages infecting a panel of Campylobacter jejuni PT14 gene knock-out mutants identified a role for the minor flagellin encoded by the flaB gene, in the defense of the host against CP8unalikevirus bacteriophage CP_F1 infection. Inactivation of the flaB gene resulted in an increase...
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Frontiers Media
2016
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| Online Access: | https://eprints.nottingham.ac.uk/38808/ |
| _version_ | 1848795695821094912 |
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| author | Lis, Lukas Connerton, Ian F. |
| author_facet | Lis, Lukas Connerton, Ian F. |
| author_sort | Lis, Lukas |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | A screen of bacteriophages infecting a panel of Campylobacter jejuni PT14 gene knock-out mutants identified a role for the minor flagellin encoded by the flaB gene, in the defense of the host against CP8unalikevirus bacteriophage CP_F1 infection. Inactivation of the flaB gene resulted in an increase in the susceptibility of PT14 cultures to infection by CP_F1 and an increase in bacteriophage yields. Infection of wild type PT14 with CP_F1 produces turbid plaques in bacterial lawns, from which 78% of the resistant isolates recovered exhibit either attenuation or complete loss of motility. CP_F1 produces clear plaques on the flaB mutant with no regrowth in the lysis zones. Complementation of the mutant restored overgrowth and the development of resistance at the expense of motility. Further analyses revealed an increase in bacteriophage adsorption constant of nearly two-fold and burst-size three-fold, relative to the wild type. Motility analysis showed no major reduction in swarming motility in the flaB mutant. Thus we propose a new role for FlaB in the defense of campylobacters against bacteriophage infection. |
| first_indexed | 2025-11-14T19:36:11Z |
| format | Article |
| id | nottingham-38808 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:36:11Z |
| publishDate | 2016 |
| publisher | Frontiers Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-388082020-05-04T18:19:42Z https://eprints.nottingham.ac.uk/38808/ The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection Lis, Lukas Connerton, Ian F. A screen of bacteriophages infecting a panel of Campylobacter jejuni PT14 gene knock-out mutants identified a role for the minor flagellin encoded by the flaB gene, in the defense of the host against CP8unalikevirus bacteriophage CP_F1 infection. Inactivation of the flaB gene resulted in an increase in the susceptibility of PT14 cultures to infection by CP_F1 and an increase in bacteriophage yields. Infection of wild type PT14 with CP_F1 produces turbid plaques in bacterial lawns, from which 78% of the resistant isolates recovered exhibit either attenuation or complete loss of motility. CP_F1 produces clear plaques on the flaB mutant with no regrowth in the lysis zones. Complementation of the mutant restored overgrowth and the development of resistance at the expense of motility. Further analyses revealed an increase in bacteriophage adsorption constant of nearly two-fold and burst-size three-fold, relative to the wild type. Motility analysis showed no major reduction in swarming motility in the flaB mutant. Thus we propose a new role for FlaB in the defense of campylobacters against bacteriophage infection. Frontiers Media 2016-11-29 Article PeerReviewed Lis, Lukas and Connerton, Ian F. (2016) The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection. Frontiers in Microbiology, 7 . p. 1908. ISSN 1664-302X Campylobacter Bacteriophages Flagellin flaB Phage escape http://journal.frontiersin.org/article/10.3389/fmicb.2016.01908/full doi:10.3389/fmicb.2016.01908 doi:10.3389/fmicb.2016.01908 |
| spellingShingle | Campylobacter Bacteriophages Flagellin flaB Phage escape Lis, Lukas Connerton, Ian F. The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection |
| title | The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection |
| title_full | The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection |
| title_fullStr | The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection |
| title_full_unstemmed | The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection |
| title_short | The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection |
| title_sort | minor flagellin of campylobacter jejuni (flab) confers defensive properties against bacteriophage infection |
| topic | Campylobacter Bacteriophages Flagellin flaB Phage escape |
| url | https://eprints.nottingham.ac.uk/38808/ https://eprints.nottingham.ac.uk/38808/ https://eprints.nottingham.ac.uk/38808/ |