Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites
IL-17 has emerged as a key player in the immune system, exhibiting roles in protection from infectious diseases and promoting inflammation in autoimmunity. Initially thought to be CD4 T-cell-derived, the sources of IL-17 are now known to be varied and belong to both the innate and adaptive arms of t...
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| Format: | Article |
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Nature Publishing Group
2014
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| Online Access: | https://eprints.nottingham.ac.uk/38085/ |
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| author | Peckham, R.K. Brill, R. Foster, David S. Bowen, A.L. Leigh, James A. Coffey, Tracey J. Flynn, Robin J. |
| author_facet | Peckham, R.K. Brill, R. Foster, David S. Bowen, A.L. Leigh, James A. Coffey, Tracey J. Flynn, Robin J. |
| author_sort | Peckham, R.K. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | IL-17 has emerged as a key player in the immune system, exhibiting roles in protection from infectious diseases and promoting inflammation in autoimmunity. Initially thought to be CD4 T-cell-derived, the sources of IL-17 are now known to be varied and belong to both the innate and adaptive arms of the immune system. Mechanisms for inducing IL-17 production in lymphoid cells are thought to rely on appropriate antigenic stimulation in the context of TGF-β1, IL-6 and/or IL-1β. Using culture protocols adapted from human studies, we have effectively induced both bovine CD4+ and WC1+ γδ T-cells to produce IL-17 termed Th17 and γδ17 cells, respectively. The negative regulatory effect of IFN-γ on mouse and human IL-17 production can be extended to the bovine model, as addition of IFN-γ decreases IL-17 production in both cell types. Furthermore we show that infection with the protozoan Neospora caninum will induce fibroblasts to secrete pro-IL-17 factors thereby inducing a γδ17 phenotype that preferentially kills infected target cells. Our study identifies two T-cell sources of IL-17, and is the first to demonstrate a protective effect of IL-17+ T-cells in ruminants. Our findings offer further opportunities for future adjuvants or vaccines which could benefit from inducing these responses. |
| first_indexed | 2025-11-14T19:34:34Z |
| format | Article |
| id | nottingham-38085 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:34:34Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-380852020-05-04T16:49:06Z https://eprints.nottingham.ac.uk/38085/ Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites Peckham, R.K. Brill, R. Foster, David S. Bowen, A.L. Leigh, James A. Coffey, Tracey J. Flynn, Robin J. IL-17 has emerged as a key player in the immune system, exhibiting roles in protection from infectious diseases and promoting inflammation in autoimmunity. Initially thought to be CD4 T-cell-derived, the sources of IL-17 are now known to be varied and belong to both the innate and adaptive arms of the immune system. Mechanisms for inducing IL-17 production in lymphoid cells are thought to rely on appropriate antigenic stimulation in the context of TGF-β1, IL-6 and/or IL-1β. Using culture protocols adapted from human studies, we have effectively induced both bovine CD4+ and WC1+ γδ T-cells to produce IL-17 termed Th17 and γδ17 cells, respectively. The negative regulatory effect of IFN-γ on mouse and human IL-17 production can be extended to the bovine model, as addition of IFN-γ decreases IL-17 production in both cell types. Furthermore we show that infection with the protozoan Neospora caninum will induce fibroblasts to secrete pro-IL-17 factors thereby inducing a γδ17 phenotype that preferentially kills infected target cells. Our study identifies two T-cell sources of IL-17, and is the first to demonstrate a protective effect of IL-17+ T-cells in ruminants. Our findings offer further opportunities for future adjuvants or vaccines which could benefit from inducing these responses. Nature Publishing Group 2014-06-25 Article PeerReviewed Peckham, R.K., Brill, R., Foster, David S., Bowen, A.L., Leigh, James A., Coffey, Tracey J. and Flynn, Robin J. (2014) Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites. Scientific Reports, 4 (5431). ISSN 2045-2322 http://www.nature.com/articles/srep05431 doi:10.1038/srep05431 doi:10.1038/srep05431 |
| spellingShingle | Peckham, R.K. Brill, R. Foster, David S. Bowen, A.L. Leigh, James A. Coffey, Tracey J. Flynn, Robin J. Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites |
| title | Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites |
| title_full | Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites |
| title_fullStr | Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites |
| title_full_unstemmed | Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites |
| title_short | Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites |
| title_sort | two distinct populations of bovine il-17+ t-cells can be induced and wc1+il-17+γδ t-cells are effective killers of protozoan parasites |
| url | https://eprints.nottingham.ac.uk/38085/ https://eprints.nottingham.ac.uk/38085/ https://eprints.nottingham.ac.uk/38085/ |