RNA expression of TLR10 in normal equine tissues

Background: Toll like receptors are one of the major innate immune system pathogen recognition systems. There is little data on the expression of the TLR10 member of this family in the horse. Results: This paper describes the genetic structure of the Equine TLR10 gene and its RNA expression in a...

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Main Authors: Tarlinton, Rachael E., Alder, Lauren, Moreton, Joanna, Maboni, G., Emes, Richard D., Tötemeyer, Sabine
Format: Article
Published: BioMed Central 2016
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Online Access:https://eprints.nottingham.ac.uk/37931/
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author Tarlinton, Rachael E.
Alder, Lauren
Moreton, Joanna
Maboni, G.
Emes, Richard D.
Tötemeyer, Sabine
author_facet Tarlinton, Rachael E.
Alder, Lauren
Moreton, Joanna
Maboni, G.
Emes, Richard D.
Tötemeyer, Sabine
author_sort Tarlinton, Rachael E.
building Nottingham Research Data Repository
collection Online Access
description Background: Toll like receptors are one of the major innate immune system pathogen recognition systems. There is little data on the expression of the TLR10 member of this family in the horse. Results: This paper describes the genetic structure of the Equine TLR10 gene and its RNA expression in a range of horse tissues. It describes the phylogenetic analysis of the Equine TLR1,6,10,2 annotations in the horse genome, firmly identifying them in their corresponding gene clades compared to other species and firmly placing the horse gene with other TLR10 genes from odd-toed ungulates. Additional 3’ transcript extensions to that annotated for TLR10 in the horse genome have been identified by analysis of RNAseq data. RNA expression of the equine TLR10 gene was highest in peripheral blood mononucleocytes and lymphoid tissue (lymph nodes and spleen), however some expression was detected in all tissues tested (jejunum, caudal mesenteric lymph nodes, bronchial lymph node, spleen, lung, colon, kidney and liver). Additional data on RNAseq expression of all equine TLR genes (1–4 and 6–10) demonstrate higher expression of TLR4 than other equine TLRs in all tissues. Conclusion: The equine TLR10 gene displays significant homology to other mammalian TLR10 genes and could be reasonably assumed to have similar fuctions. Its RNA level expression is higher in resting state PBMCs in horses than in other tissues.
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spelling nottingham-379312020-05-04T18:00:49Z https://eprints.nottingham.ac.uk/37931/ RNA expression of TLR10 in normal equine tissues Tarlinton, Rachael E. Alder, Lauren Moreton, Joanna Maboni, G. Emes, Richard D. Tötemeyer, Sabine Background: Toll like receptors are one of the major innate immune system pathogen recognition systems. There is little data on the expression of the TLR10 member of this family in the horse. Results: This paper describes the genetic structure of the Equine TLR10 gene and its RNA expression in a range of horse tissues. It describes the phylogenetic analysis of the Equine TLR1,6,10,2 annotations in the horse genome, firmly identifying them in their corresponding gene clades compared to other species and firmly placing the horse gene with other TLR10 genes from odd-toed ungulates. Additional 3’ transcript extensions to that annotated for TLR10 in the horse genome have been identified by analysis of RNAseq data. RNA expression of the equine TLR10 gene was highest in peripheral blood mononucleocytes and lymphoid tissue (lymph nodes and spleen), however some expression was detected in all tissues tested (jejunum, caudal mesenteric lymph nodes, bronchial lymph node, spleen, lung, colon, kidney and liver). Additional data on RNAseq expression of all equine TLR genes (1–4 and 6–10) demonstrate higher expression of TLR4 than other equine TLRs in all tissues. Conclusion: The equine TLR10 gene displays significant homology to other mammalian TLR10 genes and could be reasonably assumed to have similar fuctions. Its RNA level expression is higher in resting state PBMCs in horses than in other tissues. BioMed Central 2016-07-19 Article PeerReviewed Tarlinton, Rachael E., Alder, Lauren, Moreton, Joanna, Maboni, G., Emes, Richard D. and Tötemeyer, Sabine (2016) RNA expression of TLR10 in normal equine tissues. BMC Research Notes, 9 . 353/1-353/8. ISSN 1756-0500 TLR10 Toll-like receptor Horse Equus caballus RNA http://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-016-2161-9 doi:10.1186/s13104-016-2161-9 doi:10.1186/s13104-016-2161-9
spellingShingle TLR10
Toll-like receptor
Horse
Equus caballus
RNA
Tarlinton, Rachael E.
Alder, Lauren
Moreton, Joanna
Maboni, G.
Emes, Richard D.
Tötemeyer, Sabine
RNA expression of TLR10 in normal equine tissues
title RNA expression of TLR10 in normal equine tissues
title_full RNA expression of TLR10 in normal equine tissues
title_fullStr RNA expression of TLR10 in normal equine tissues
title_full_unstemmed RNA expression of TLR10 in normal equine tissues
title_short RNA expression of TLR10 in normal equine tissues
title_sort rna expression of tlr10 in normal equine tissues
topic TLR10
Toll-like receptor
Horse
Equus caballus
RNA
url https://eprints.nottingham.ac.uk/37931/
https://eprints.nottingham.ac.uk/37931/
https://eprints.nottingham.ac.uk/37931/