PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus

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PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus

The Pragmatic Insertional Mutation Mapping (PIMMS) laboratory protocol was developed alongside various bioinformatics packages (Blanchard et al., 2015) to enable detection of essential and conditionally essential genes in Streptococcus and related bacteria. This extended the methodology commonly use...

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Bibliographic Details
Main Authors:	Blanchard, Adam M., Egan, Sharon A., Emes, Richard D., Warry, Andrew, Leigh, James A.
Format:	Article
Published:	Frontiers 2016
Subjects:	mutagenesis insertion sequencing essential genome Streptococcus laboratory protocol
Online Access:	https://eprints.nottingham.ac.uk/37925/

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