Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom
Increasing evidence suggests that a new birnavirus, named Chicken proventricular necrosis virus (CPNV), is the aetiological agent of transmissible viral proventriculitis (TVP). The present work aimed to explore the possible presence of both TVP and CPNV in the UK. Forty-four chickens showing TVP-com...
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| Format: | Article |
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Taylor and Francis
2017
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| Online Access: | https://eprints.nottingham.ac.uk/37924/ |
| _version_ | 1848795561697738752 |
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| author | Grau-Roma, Llorenc Reid, Kirsty de Brot, Simone Jennison, Richard Barrow, Paul Sánchez, Raúl Nofrarías, Miquel Clark, Mike Majó, Natàlia |
| author_facet | Grau-Roma, Llorenc Reid, Kirsty de Brot, Simone Jennison, Richard Barrow, Paul Sánchez, Raúl Nofrarías, Miquel Clark, Mike Majó, Natàlia |
| author_sort | Grau-Roma, Llorenc |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Increasing evidence suggests that a new birnavirus, named Chicken proventricular necrosis virus (CPNV), is the aetiological agent of transmissible viral proventriculitis (TVP). The present work aimed to explore the possible presence of both TVP and CPNV in the UK. Forty-four chickens showing TVP-compatible gross lesions were classified into 3 groups based on the histological lesions: i) TVP-affected chickens: lymphocytic infiltration and glandular necrosis (n=15); ii) lymphocytic proventriculitis (LP)-affected chickens: lymphocytic infiltration without necrosis (n=18); and iii) without proventriculitis (WP): no lymphocytic infiltration or necrosis (n=11). Nine proventriculi (7 out of 15 corresponding to TVP, and 2 out of 11 corresponding to LP) were positive for CPNV by RT-PCR. These results support the previously suggested idea of CPNV as causative agent of TVP. Moreover, this data shows that CPNV can also be detected in a number of cases with LP, which do not fulfil the histological TVP criteria. Phylogenetic analysis of partial sequences of gene VP1 showed that British CPNV sequences were closer to other European CPNV sequences and might constitute a different lineage from the American CPNV. TVP cases with negative CPNV PCR results may be due to chronic stages of the disease or to the reduced PCR sensitivity on formalin-fixed paraffin embedded tissues. However, involvement of other agents in some of the cases cannot totally be ruled out. As far as the authors are aware, this is the first peer-reviewed report of TVP as well as of CPNV in the UK, and the first exploratory CPNV phylogenetic study. |
| first_indexed | 2025-11-14T19:34:03Z |
| format | Article |
| id | nottingham-37924 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:34:03Z |
| publishDate | 2017 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-379242020-05-04T19:59:20Z https://eprints.nottingham.ac.uk/37924/ Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom Grau-Roma, Llorenc Reid, Kirsty de Brot, Simone Jennison, Richard Barrow, Paul Sánchez, Raúl Nofrarías, Miquel Clark, Mike Majó, Natàlia Increasing evidence suggests that a new birnavirus, named Chicken proventricular necrosis virus (CPNV), is the aetiological agent of transmissible viral proventriculitis (TVP). The present work aimed to explore the possible presence of both TVP and CPNV in the UK. Forty-four chickens showing TVP-compatible gross lesions were classified into 3 groups based on the histological lesions: i) TVP-affected chickens: lymphocytic infiltration and glandular necrosis (n=15); ii) lymphocytic proventriculitis (LP)-affected chickens: lymphocytic infiltration without necrosis (n=18); and iii) without proventriculitis (WP): no lymphocytic infiltration or necrosis (n=11). Nine proventriculi (7 out of 15 corresponding to TVP, and 2 out of 11 corresponding to LP) were positive for CPNV by RT-PCR. These results support the previously suggested idea of CPNV as causative agent of TVP. Moreover, this data shows that CPNV can also be detected in a number of cases with LP, which do not fulfil the histological TVP criteria. Phylogenetic analysis of partial sequences of gene VP1 showed that British CPNV sequences were closer to other European CPNV sequences and might constitute a different lineage from the American CPNV. TVP cases with negative CPNV PCR results may be due to chronic stages of the disease or to the reduced PCR sensitivity on formalin-fixed paraffin embedded tissues. However, involvement of other agents in some of the cases cannot totally be ruled out. As far as the authors are aware, this is the first peer-reviewed report of TVP as well as of CPNV in the UK, and the first exploratory CPNV phylogenetic study. Taylor and Francis 2017 Article PeerReviewed Grau-Roma, Llorenc, Reid, Kirsty, de Brot, Simone, Jennison, Richard, Barrow, Paul, Sánchez, Raúl, Nofrarías, Miquel, Clark, Mike and Majó, Natàlia (2017) Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom. Avian Pathology, 46 (1). pp. 1-25. ISSN 0307-9457 http://www.tandfonline.com/doi/abs/10.1080/03079457.2016.1207751 doi:10.1080/03079457.2016.1207751 doi:10.1080/03079457.2016.1207751 |
| spellingShingle | Grau-Roma, Llorenc Reid, Kirsty de Brot, Simone Jennison, Richard Barrow, Paul Sánchez, Raúl Nofrarías, Miquel Clark, Mike Majó, Natàlia Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom |
| title | Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom |
| title_full | Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom |
| title_fullStr | Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom |
| title_full_unstemmed | Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom |
| title_short | Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom |
| title_sort | detection of transmissible viral proventriculitis (tvp) and chicken proventricular necrosis virus (cpnv) in the united kingdom |
| url | https://eprints.nottingham.ac.uk/37924/ https://eprints.nottingham.ac.uk/37924/ https://eprints.nottingham.ac.uk/37924/ |