Plant embryogenesis requires AUX/LAX-mediated auxin influx
The plant hormone auxin and its directional transport are known to play a crucial role in defining the embryonic axis and subsequent development of the body plan. Although the role of PIN auxin efflux transporters has been clearly assigned during embryonic shoot and root specification, the role of t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Company of Biologists
2015
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| Online Access: | https://eprints.nottingham.ac.uk/37865/ |
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| author | Robert, Hélène S Grunewald, Wim Sauer, Michael Cannoot, Bernard Soriano, Mercedes Swarup, Ranjan Weijers, Dolf Bennett, Malcolm J. Boutilier, Kim Friml, Jiri |
| author_facet | Robert, Hélène S Grunewald, Wim Sauer, Michael Cannoot, Bernard Soriano, Mercedes Swarup, Ranjan Weijers, Dolf Bennett, Malcolm J. Boutilier, Kim Friml, Jiri |
| author_sort | Robert, Hélène S |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The plant hormone auxin and its directional transport are known to play a crucial role in defining the embryonic axis and subsequent development of the body plan. Although the role of PIN auxin efflux transporters has been clearly assigned during embryonic shoot and root specification, the role of the auxin influx carriers AUX1 and LIKE-AUX1 (LAX) proteins is not well established. Here, we used chemical and genetic tools on Brassica napus microspore-derived embryos and Arabidopsis thaliana zygotic embryos, and demonstrate that AUX1, LAX1 and LAX2 are required for both shoot and root pole formation, in concert with PIN efflux carriers. Furthermore, we uncovered a positive-feedback loop between MONOPTEROS (ARF5)-dependent auxin signalling and auxin transport. This MONOPTEROS-dependent transcriptional regulation of auxin influx (AUX1, LAX1 and LAX2) and auxin efflux (PIN1 and PIN4) carriers by MONOPTEROS helps to maintain proper auxin transport to the root tip. These results indicate that auxin-dependent cell specification during embryo development requires balanced auxin transport involving both influx and efflux mechanisms, and that this transport is maintained by a positive transcriptional feedback on auxin signalling. |
| first_indexed | 2025-11-14T19:33:52Z |
| format | Article |
| id | nottingham-37865 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:33:52Z |
| publishDate | 2015 |
| publisher | Company of Biologists |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-378652020-05-04T17:02:39Z https://eprints.nottingham.ac.uk/37865/ Plant embryogenesis requires AUX/LAX-mediated auxin influx Robert, Hélène S Grunewald, Wim Sauer, Michael Cannoot, Bernard Soriano, Mercedes Swarup, Ranjan Weijers, Dolf Bennett, Malcolm J. Boutilier, Kim Friml, Jiri The plant hormone auxin and its directional transport are known to play a crucial role in defining the embryonic axis and subsequent development of the body plan. Although the role of PIN auxin efflux transporters has been clearly assigned during embryonic shoot and root specification, the role of the auxin influx carriers AUX1 and LIKE-AUX1 (LAX) proteins is not well established. Here, we used chemical and genetic tools on Brassica napus microspore-derived embryos and Arabidopsis thaliana zygotic embryos, and demonstrate that AUX1, LAX1 and LAX2 are required for both shoot and root pole formation, in concert with PIN efflux carriers. Furthermore, we uncovered a positive-feedback loop between MONOPTEROS (ARF5)-dependent auxin signalling and auxin transport. This MONOPTEROS-dependent transcriptional regulation of auxin influx (AUX1, LAX1 and LAX2) and auxin efflux (PIN1 and PIN4) carriers by MONOPTEROS helps to maintain proper auxin transport to the root tip. These results indicate that auxin-dependent cell specification during embryo development requires balanced auxin transport involving both influx and efflux mechanisms, and that this transport is maintained by a positive transcriptional feedback on auxin signalling. Company of Biologists 2015-02-15 Article PeerReviewed Robert, Hélène S, Grunewald, Wim, Sauer, Michael, Cannoot, Bernard, Soriano, Mercedes, Swarup, Ranjan, Weijers, Dolf, Bennett, Malcolm J., Boutilier, Kim and Friml, Jiri (2015) Plant embryogenesis requires AUX/LAX-mediated auxin influx. Development, 142 (4). pp. 702-711. ISSN 1477-9129 Arabidopsis thaliana embryogenesis Auxin transport AUX1 LIKE-AUX1 (LAX) MONOPTEROS (ARF5) PIN Brassica napus Microspore http://dev.biologists.org/content/142/4/702 doi:10.1242/dev.115832 doi:10.1242/dev.115832 |
| spellingShingle | Arabidopsis thaliana embryogenesis Auxin transport AUX1 LIKE-AUX1 (LAX) MONOPTEROS (ARF5) PIN Brassica napus Microspore Robert, Hélène S Grunewald, Wim Sauer, Michael Cannoot, Bernard Soriano, Mercedes Swarup, Ranjan Weijers, Dolf Bennett, Malcolm J. Boutilier, Kim Friml, Jiri Plant embryogenesis requires AUX/LAX-mediated auxin influx |
| title | Plant embryogenesis requires AUX/LAX-mediated auxin influx |
| title_full | Plant embryogenesis requires AUX/LAX-mediated auxin influx |
| title_fullStr | Plant embryogenesis requires AUX/LAX-mediated auxin influx |
| title_full_unstemmed | Plant embryogenesis requires AUX/LAX-mediated auxin influx |
| title_short | Plant embryogenesis requires AUX/LAX-mediated auxin influx |
| title_sort | plant embryogenesis requires aux/lax-mediated auxin influx |
| topic | Arabidopsis thaliana embryogenesis Auxin transport AUX1 LIKE-AUX1 (LAX) MONOPTEROS (ARF5) PIN Brassica napus Microspore |
| url | https://eprints.nottingham.ac.uk/37865/ https://eprints.nottingham.ac.uk/37865/ https://eprints.nottingham.ac.uk/37865/ |