Lateral root emergence in Arabidopsisis dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3
Lateral root primordia (LRP) originate from pericycle stem cells located deep within parental root tissues. LRP emerge through overlying root tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells. The auxin-inducible auxin influx carrier LAX3 plays a key role co...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
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Company of Biologists
2016
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| Online Access: | https://eprints.nottingham.ac.uk/37859/ |
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| author | Porco, Silvana Larrieu, Antoine Du, Yujuan Gaudinier, Allison Goh, Tatsuaki Swarup, Kamal Swarup, Ranjan Kuempers, Britta Bishopp, Anthony Lavenus, Julien Casimiro, Ilda Hill, Kristine Benkova, Eva Fukaki, Hidehiro Brady, Siobhan M. Scheres, Ben Peret, Benjamin Bennett, Malcolm J. |
| author_facet | Porco, Silvana Larrieu, Antoine Du, Yujuan Gaudinier, Allison Goh, Tatsuaki Swarup, Kamal Swarup, Ranjan Kuempers, Britta Bishopp, Anthony Lavenus, Julien Casimiro, Ilda Hill, Kristine Benkova, Eva Fukaki, Hidehiro Brady, Siobhan M. Scheres, Ben Peret, Benjamin Bennett, Malcolm J. |
| author_sort | Porco, Silvana |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Lateral root primordia (LRP) originate from pericycle stem cells located deep within parental root tissues. LRP emerge through overlying root tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells. The auxin-inducible auxin influx carrier LAX3 plays a key role concentrating this signal in cells overlying LRP. Delimiting LAX3 expression to two adjacent cell files overlying new LRP is crucial to ensure that auxin-regulated cell separation occurs solely along their shared walls. Multiscale modeling has predicted that this highly focused pattern of expression requires auxin to sequentially induce auxin efflux and influx carriers PIN3 and LAX3, respectively. Consistent with model predictions, we report that auxin-inducible LAX3 expression is regulated indirectly by AUXIN RESPONSE FACTOR 7 (ARF7). Yeast one-hybrid screens revealed that the LAX3 promoter is bound by the transcription factor LBD29, which is a direct target for regulation by ARF7. Disrupting auxin-inducible LBD29 expression or expressing an LBD29-SRDX transcriptional repressor phenocopied the lax3 mutant, resulting in delayed lateral root emergence. We conclude that sequential LBD29 and LAX3 induction by auxin is required to coordinate cell separation and organ emergence. |
| first_indexed | 2025-11-14T19:33:51Z |
| format | Article |
| id | nottingham-37859 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:33:51Z |
| publishDate | 2016 |
| publisher | Company of Biologists |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-378592020-05-04T18:05:25Z https://eprints.nottingham.ac.uk/37859/ Lateral root emergence in Arabidopsisis dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3 Porco, Silvana Larrieu, Antoine Du, Yujuan Gaudinier, Allison Goh, Tatsuaki Swarup, Kamal Swarup, Ranjan Kuempers, Britta Bishopp, Anthony Lavenus, Julien Casimiro, Ilda Hill, Kristine Benkova, Eva Fukaki, Hidehiro Brady, Siobhan M. Scheres, Ben Peret, Benjamin Bennett, Malcolm J. Lateral root primordia (LRP) originate from pericycle stem cells located deep within parental root tissues. LRP emerge through overlying root tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells. The auxin-inducible auxin influx carrier LAX3 plays a key role concentrating this signal in cells overlying LRP. Delimiting LAX3 expression to two adjacent cell files overlying new LRP is crucial to ensure that auxin-regulated cell separation occurs solely along their shared walls. Multiscale modeling has predicted that this highly focused pattern of expression requires auxin to sequentially induce auxin efflux and influx carriers PIN3 and LAX3, respectively. Consistent with model predictions, we report that auxin-inducible LAX3 expression is regulated indirectly by AUXIN RESPONSE FACTOR 7 (ARF7). Yeast one-hybrid screens revealed that the LAX3 promoter is bound by the transcription factor LBD29, which is a direct target for regulation by ARF7. Disrupting auxin-inducible LBD29 expression or expressing an LBD29-SRDX transcriptional repressor phenocopied the lax3 mutant, resulting in delayed lateral root emergence. We conclude that sequential LBD29 and LAX3 induction by auxin is required to coordinate cell separation and organ emergence. Company of Biologists 2016-08-30 Article PeerReviewed Porco, Silvana, Larrieu, Antoine, Du, Yujuan, Gaudinier, Allison, Goh, Tatsuaki, Swarup, Kamal, Swarup, Ranjan, Kuempers, Britta, Bishopp, Anthony, Lavenus, Julien, Casimiro, Ilda, Hill, Kristine, Benkova, Eva, Fukaki, Hidehiro, Brady, Siobhan M., Scheres, Ben, Peret, Benjamin and Bennett, Malcolm J. (2016) Lateral root emergence in Arabidopsisis dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3. Development, 143 (18). pp. 3340-3349. ISSN 1477-9129 Arabidopsis; Root development; Lateral root emergence; Auxin; LBD29 http://dx.doi.org/10.1242/dev.136283 doi:10.1242/dev.136283 doi:10.1242/dev.136283 |
| spellingShingle | Arabidopsis; Root development; Lateral root emergence; Auxin; LBD29 Porco, Silvana Larrieu, Antoine Du, Yujuan Gaudinier, Allison Goh, Tatsuaki Swarup, Kamal Swarup, Ranjan Kuempers, Britta Bishopp, Anthony Lavenus, Julien Casimiro, Ilda Hill, Kristine Benkova, Eva Fukaki, Hidehiro Brady, Siobhan M. Scheres, Ben Peret, Benjamin Bennett, Malcolm J. Lateral root emergence in Arabidopsisis dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3 |
| title | Lateral root emergence in Arabidopsisis dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3 |
| title_full | Lateral root emergence in Arabidopsisis dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3 |
| title_fullStr | Lateral root emergence in Arabidopsisis dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3 |
| title_full_unstemmed | Lateral root emergence in Arabidopsisis dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3 |
| title_short | Lateral root emergence in Arabidopsisis dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3 |
| title_sort | lateral root emergence in arabidopsisis dependent on transcription factor lbd29 regulation of auxin influx carrier lax3 |
| topic | Arabidopsis; Root development; Lateral root emergence; Auxin; LBD29 |
| url | https://eprints.nottingham.ac.uk/37859/ https://eprints.nottingham.ac.uk/37859/ https://eprints.nottingham.ac.uk/37859/ |