Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?

Muscular dystrophies (MDs) are clinically and molecularly a highly heterogeneous group of single-gene disorders that primarily affect striated muscles. Cardiac disease is present in several MDs where it is an important contributor to morbidity and mortality. Careful monitoring of cardiac issues is n...

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Main Authors: Kalra, Spandan, Montanaro, Federica, Denning, Chris
Format: Article
Published: IOS Press 2016
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Online Access:https://eprints.nottingham.ac.uk/37782/
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author Kalra, Spandan
Montanaro, Federica
Denning, Chris
author_facet Kalra, Spandan
Montanaro, Federica
Denning, Chris
author_sort Kalra, Spandan
building Nottingham Research Data Repository
collection Online Access
description Muscular dystrophies (MDs) are clinically and molecularly a highly heterogeneous group of single-gene disorders that primarily affect striated muscles. Cardiac disease is present in several MDs where it is an important contributor to morbidity and mortality. Careful monitoring of cardiac issues is necessary but current management of cardiac involvement does not effectively protect from disease progression and cardiac failure. There is a critical need to gain new knowledge on the diverse molecular underpinnings of cardiac disease in MDs in order to guide cardiac treatment development and assist in reaching a clearer consensus on cardiac disease management in the clinic. Animal models are available for the majority of MDs and have been invaluable tools in probing disease mechanisms and in pre-clinical screens. However, there are recognized genetic, physiological, and structural differences between human and animal hearts that impact disease progression, manifestation, and response to pharmacological interventions. Therefore, there is a need to develop parallel human systems to model cardiac disease in MDs. This review discusses the current status of cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSC) to model cardiac disease, with a focus on Duchenne muscular dystrophy (DMD) and myotonic dystrophy (DM1). We seek to provide a balanced view of opportunities and limitations offered by this system in elucidating disease mechanisms pertinent to human cardiac physiology and as a platform for treatment development or refinement.
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spelling nottingham-377822020-05-04T17:58:26Z https://eprints.nottingham.ac.uk/37782/ Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies? Kalra, Spandan Montanaro, Federica Denning, Chris Muscular dystrophies (MDs) are clinically and molecularly a highly heterogeneous group of single-gene disorders that primarily affect striated muscles. Cardiac disease is present in several MDs where it is an important contributor to morbidity and mortality. Careful monitoring of cardiac issues is necessary but current management of cardiac involvement does not effectively protect from disease progression and cardiac failure. There is a critical need to gain new knowledge on the diverse molecular underpinnings of cardiac disease in MDs in order to guide cardiac treatment development and assist in reaching a clearer consensus on cardiac disease management in the clinic. Animal models are available for the majority of MDs and have been invaluable tools in probing disease mechanisms and in pre-clinical screens. However, there are recognized genetic, physiological, and structural differences between human and animal hearts that impact disease progression, manifestation, and response to pharmacological interventions. Therefore, there is a need to develop parallel human systems to model cardiac disease in MDs. This review discusses the current status of cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSC) to model cardiac disease, with a focus on Duchenne muscular dystrophy (DMD) and myotonic dystrophy (DM1). We seek to provide a balanced view of opportunities and limitations offered by this system in elucidating disease mechanisms pertinent to human cardiac physiology and as a platform for treatment development or refinement. IOS Press 2016-08-01 Article PeerReviewed Kalra, Spandan, Montanaro, Federica and Denning, Chris (2016) Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies? Journal of Neuromuscular Diseases, 3 (3). pp. 309-332. ISSN 2214-3602 Human embryonic stem cells human induced pluripotent stem cells Cas9/CRISPR genome editing cardiomyocytes Duchenne muscular dystrophy (DMD) myotonic dystrophy (DM1) disease modelling exon skipping gene therapy http://content.iospress.com/articles/journal-of-neuromuscular-diseases/jnd150133 doi:10.3233/JND-150133 doi:10.3233/JND-150133
spellingShingle Human embryonic stem cells
human induced pluripotent stem cells
Cas9/CRISPR genome editing
cardiomyocytes
Duchenne muscular dystrophy (DMD)
myotonic dystrophy (DM1)
disease modelling
exon skipping
gene therapy
Kalra, Spandan
Montanaro, Federica
Denning, Chris
Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?
title Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?
title_full Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?
title_fullStr Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?
title_full_unstemmed Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?
title_short Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?
title_sort can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?
topic Human embryonic stem cells
human induced pluripotent stem cells
Cas9/CRISPR genome editing
cardiomyocytes
Duchenne muscular dystrophy (DMD)
myotonic dystrophy (DM1)
disease modelling
exon skipping
gene therapy
url https://eprints.nottingham.ac.uk/37782/
https://eprints.nottingham.ac.uk/37782/
https://eprints.nottingham.ac.uk/37782/