Real-time selective sequencing using nanopore technology
The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstra...
| Main Authors: | , , |
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| Format: | Article |
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Nature Publishing Group
2016
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| Online Access: | https://eprints.nottingham.ac.uk/37695/ |
| _version_ | 1848795513710706688 |
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| author | Loose, Matthew Malla, Sunir Stout, Michael |
| author_facet | Loose, Matthew Malla, Sunir Stout, Michael |
| author_sort | Loose, Matthew |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set. |
| first_indexed | 2025-11-14T19:33:17Z |
| format | Article |
| id | nottingham-37695 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:33:17Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-376952020-05-04T17:59:50Z https://eprints.nottingham.ac.uk/37695/ Real-time selective sequencing using nanopore technology Loose, Matthew Malla, Sunir Stout, Michael The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set. Nature Publishing Group 2016-07-25 Article PeerReviewed Loose, Matthew, Malla, Sunir and Stout, Michael (2016) Real-time selective sequencing using nanopore technology. Nature Methods, 13 (9). pp. 751-754. ISSN 1548-7105 http://www.nature.com/nmeth/journal/v13/n9/full/nmeth.3930.html doi:10.1038/nmeth.3930 doi:10.1038/nmeth.3930 |
| spellingShingle | Loose, Matthew Malla, Sunir Stout, Michael Real-time selective sequencing using nanopore technology |
| title | Real-time selective sequencing using nanopore technology |
| title_full | Real-time selective sequencing using nanopore technology |
| title_fullStr | Real-time selective sequencing using nanopore technology |
| title_full_unstemmed | Real-time selective sequencing using nanopore technology |
| title_short | Real-time selective sequencing using nanopore technology |
| title_sort | real-time selective sequencing using nanopore technology |
| url | https://eprints.nottingham.ac.uk/37695/ https://eprints.nottingham.ac.uk/37695/ https://eprints.nottingham.ac.uk/37695/ |