Gene pathway development in human epicardial adipose tissue during early life

Studies in rodents and newborn humans demonstrate the influence of brown adipose tissue (BAT) in temperature control and energy balance and a critical role in the regulation of body weight. Here, we obtained samples of epicardial adipose tissue (EAT) from neonates, infants, and children in order to...

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Main Authors: Ojha, Shalini, Fainberg, Hernan P., Wilson, Victoria, Pelella, Giuseppe, Castellanos, Marcos, May, Sean, Lotto, Attilio A., Sacks, Harold, Symonds, Michael E., Budge, Helen
Format: Article
Published: American Society for Clinical Investigation 2016
Online Access:https://eprints.nottingham.ac.uk/37678/
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author Ojha, Shalini
Fainberg, Hernan P.
Wilson, Victoria
Pelella, Giuseppe
Castellanos, Marcos
May, Sean
Lotto, Attilio A.
Sacks, Harold
Symonds, Michael E.
Budge, Helen
author_facet Ojha, Shalini
Fainberg, Hernan P.
Wilson, Victoria
Pelella, Giuseppe
Castellanos, Marcos
May, Sean
Lotto, Attilio A.
Sacks, Harold
Symonds, Michael E.
Budge, Helen
author_sort Ojha, Shalini
building Nottingham Research Data Repository
collection Online Access
description Studies in rodents and newborn humans demonstrate the influence of brown adipose tissue (BAT) in temperature control and energy balance and a critical role in the regulation of body weight. Here, we obtained samples of epicardial adipose tissue (EAT) from neonates, infants, and children in order to evaluate changes in their transcriptional landscape by applying a systems biology approach. Surprisingly, these analyses revealed that the transition to infancy is a critical stage for changes in the morphology of EAT and is reflected in unique gene expression patterns of a substantial proportion of thermogenic gene transcripts (~10%). Our results also indicated that the pattern of gene expression represents a distinct developmental stage, even after the rebound in abundance of thermogenic genes in later childhood. Using weighted gene coexpression network analyses, we found precise anthropometric-specific correlations with changes in gene expression and the decline of thermogenic capacity within EAT. In addition, these results indicate a sequential order of transcriptional events affecting cellular pathways, which could potentially explain the variation in the amount, or activity, of BAT in adulthood. Together, these results provide a resource to elucidate gene regulatory mechanisms underlying the progressive development of BAT during early life.
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spelling nottingham-376782020-05-04T18:06:36Z https://eprints.nottingham.ac.uk/37678/ Gene pathway development in human epicardial adipose tissue during early life Ojha, Shalini Fainberg, Hernan P. Wilson, Victoria Pelella, Giuseppe Castellanos, Marcos May, Sean Lotto, Attilio A. Sacks, Harold Symonds, Michael E. Budge, Helen Studies in rodents and newborn humans demonstrate the influence of brown adipose tissue (BAT) in temperature control and energy balance and a critical role in the regulation of body weight. Here, we obtained samples of epicardial adipose tissue (EAT) from neonates, infants, and children in order to evaluate changes in their transcriptional landscape by applying a systems biology approach. Surprisingly, these analyses revealed that the transition to infancy is a critical stage for changes in the morphology of EAT and is reflected in unique gene expression patterns of a substantial proportion of thermogenic gene transcripts (~10%). Our results also indicated that the pattern of gene expression represents a distinct developmental stage, even after the rebound in abundance of thermogenic genes in later childhood. Using weighted gene coexpression network analyses, we found precise anthropometric-specific correlations with changes in gene expression and the decline of thermogenic capacity within EAT. In addition, these results indicate a sequential order of transcriptional events affecting cellular pathways, which could potentially explain the variation in the amount, or activity, of BAT in adulthood. Together, these results provide a resource to elucidate gene regulatory mechanisms underlying the progressive development of BAT during early life. American Society for Clinical Investigation 2016-08-18 Article PeerReviewed Ojha, Shalini, Fainberg, Hernan P., Wilson, Victoria, Pelella, Giuseppe, Castellanos, Marcos, May, Sean, Lotto, Attilio A., Sacks, Harold, Symonds, Michael E. and Budge, Helen (2016) Gene pathway development in human epicardial adipose tissue during early life. JCI Insight, 1 (13). e87460/1-e87460/13. ISSN 2379-3708 https://insight.jci.org/articles/view/87460 doi:10.1172/jci.insight.87460 doi:10.1172/jci.insight.87460
spellingShingle Ojha, Shalini
Fainberg, Hernan P.
Wilson, Victoria
Pelella, Giuseppe
Castellanos, Marcos
May, Sean
Lotto, Attilio A.
Sacks, Harold
Symonds, Michael E.
Budge, Helen
Gene pathway development in human epicardial adipose tissue during early life
title Gene pathway development in human epicardial adipose tissue during early life
title_full Gene pathway development in human epicardial adipose tissue during early life
title_fullStr Gene pathway development in human epicardial adipose tissue during early life
title_full_unstemmed Gene pathway development in human epicardial adipose tissue during early life
title_short Gene pathway development in human epicardial adipose tissue during early life
title_sort gene pathway development in human epicardial adipose tissue during early life
url https://eprints.nottingham.ac.uk/37678/
https://eprints.nottingham.ac.uk/37678/
https://eprints.nottingham.ac.uk/37678/