Metabolomics of aging assessed in individual parasitoid wasps
Metabolomics studies of low-biomass organisms, such as small insects, have previously relied on the pooling of biological samples to overcome detection limits, particularly using NMR. We show that the differentiation of metabolite profiles of individual 1 mg parasitoid wasps of different ages is pos...
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| Format: | Article |
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Nature Publishing Group
2016
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| Online Access: | https://eprints.nottingham.ac.uk/37662/ |
| _version_ | 1848795506040373248 |
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| author | Kapranas, Apostolos Snart, Charles J.P. Williams, Huw Edward Llewelyn Hardy, Ian C.W. Barrett, David A. |
| author_facet | Kapranas, Apostolos Snart, Charles J.P. Williams, Huw Edward Llewelyn Hardy, Ian C.W. Barrett, David A. |
| author_sort | Kapranas, Apostolos |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Metabolomics studies of low-biomass organisms, such as small insects, have previously relied on the pooling of biological samples to overcome detection limits, particularly using NMR. We show that the differentiation of metabolite profiles of individual 1 mg parasitoid wasps of different ages is possible when using a modified sample preparation and a combination of untargeted NMR and LC-MS based metabolomics. Changes were observed between newly emerged and older wasps in glycerolipids, amino acids and circulatory sugars. This advance in chemical profiling has important implications for the study of the behaviour and ecology of parasitoids and many other species of small organisms because predictions and observations are typically made at the level of the individual. Thus, the metabolomic state of low-biomass individuals can now be related to their behaviour and ecological performance. We discuss specifically the utility of age-related metabolomic profiling but our new approach can be applied to a wide range of biological research. |
| first_indexed | 2025-11-14T19:33:10Z |
| format | Article |
| id | nottingham-37662 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:33:10Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-376622020-05-04T18:17:42Z https://eprints.nottingham.ac.uk/37662/ Metabolomics of aging assessed in individual parasitoid wasps Kapranas, Apostolos Snart, Charles J.P. Williams, Huw Edward Llewelyn Hardy, Ian C.W. Barrett, David A. Metabolomics studies of low-biomass organisms, such as small insects, have previously relied on the pooling of biological samples to overcome detection limits, particularly using NMR. We show that the differentiation of metabolite profiles of individual 1 mg parasitoid wasps of different ages is possible when using a modified sample preparation and a combination of untargeted NMR and LC-MS based metabolomics. Changes were observed between newly emerged and older wasps in glycerolipids, amino acids and circulatory sugars. This advance in chemical profiling has important implications for the study of the behaviour and ecology of parasitoids and many other species of small organisms because predictions and observations are typically made at the level of the individual. Thus, the metabolomic state of low-biomass individuals can now be related to their behaviour and ecological performance. We discuss specifically the utility of age-related metabolomic profiling but our new approach can be applied to a wide range of biological research. Nature Publishing Group 2016-10-07 Article PeerReviewed Kapranas, Apostolos, Snart, Charles J.P., Williams, Huw Edward Llewelyn, Hardy, Ian C.W. and Barrett, David A. (2016) Metabolomics of aging assessed in individual parasitoid wasps. Scientific Reports, 6 . p. 34848. ISSN 2045-2322 Ageing; behavioural ecology; chemical ecology; lipidomics; metabolomics http://www.nature.com/articles/srep34848 doi:10.1038/srep34848 doi:10.1038/srep34848 |
| spellingShingle | Ageing; behavioural ecology; chemical ecology; lipidomics; metabolomics Kapranas, Apostolos Snart, Charles J.P. Williams, Huw Edward Llewelyn Hardy, Ian C.W. Barrett, David A. Metabolomics of aging assessed in individual parasitoid wasps |
| title | Metabolomics of aging assessed in individual parasitoid wasps |
| title_full | Metabolomics of aging assessed in individual parasitoid wasps |
| title_fullStr | Metabolomics of aging assessed in individual parasitoid wasps |
| title_full_unstemmed | Metabolomics of aging assessed in individual parasitoid wasps |
| title_short | Metabolomics of aging assessed in individual parasitoid wasps |
| title_sort | metabolomics of aging assessed in individual parasitoid wasps |
| topic | Ageing; behavioural ecology; chemical ecology; lipidomics; metabolomics |
| url | https://eprints.nottingham.ac.uk/37662/ https://eprints.nottingham.ac.uk/37662/ https://eprints.nottingham.ac.uk/37662/ |