Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif
Sortase (a transamidase) has been shown to be responsible for the covalent attachment of proteins to the bacterial cell wall. Anchoring is effected on secreted proteins containing a specific cell wall motif toward their C-terminus; that for sortase A (SrtA) in Gram-positive bacteria often incorporat...
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| Format: | Article |
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American Chemical Society
2009
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| Online Access: | https://eprints.nottingham.ac.uk/37634/ |
| _version_ | 1848795502070464512 |
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| author | Egan, Sharon A. Kurian, Dominic Ward, Philip N. Hunt, Lawrence Leigh, James A. |
| author_facet | Egan, Sharon A. Kurian, Dominic Ward, Philip N. Hunt, Lawrence Leigh, James A. |
| author_sort | Egan, Sharon A. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Sortase (a transamidase) has been shown to be responsible for the covalent attachment of proteins to the bacterial cell wall. Anchoring is effected on secreted proteins containing a specific cell wall motif toward their C-terminus; that for sortase A (SrtA) in Gram-positive bacteria often incorporates the sequence LPXTG. Such surface proteins are often characterized as virulence determinants and play important roles during the establishment and persistence of infection. Intramammary infection with Streptococcus uberis is a common cause of bovine mastitis, which impacts on animal health and welfare and the economics of milk production. Comparison of stringently produced cell wall fractions from S. uberis and an isogenic mutant strain lacking SrtA permitted identification of 9 proteins likely to be covalently anchored at the cell surface. Analysis of these sequences implied the presence of two anchoring motifs for S. uberis, the classical LPXTG motif and an additional LPXXXD motif. |
| first_indexed | 2025-11-14T19:33:06Z |
| format | Article |
| id | nottingham-37634 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:33:06Z |
| publishDate | 2009 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-376342020-05-04T16:28:49Z https://eprints.nottingham.ac.uk/37634/ Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif Egan, Sharon A. Kurian, Dominic Ward, Philip N. Hunt, Lawrence Leigh, James A. Sortase (a transamidase) has been shown to be responsible for the covalent attachment of proteins to the bacterial cell wall. Anchoring is effected on secreted proteins containing a specific cell wall motif toward their C-terminus; that for sortase A (SrtA) in Gram-positive bacteria often incorporates the sequence LPXTG. Such surface proteins are often characterized as virulence determinants and play important roles during the establishment and persistence of infection. Intramammary infection with Streptococcus uberis is a common cause of bovine mastitis, which impacts on animal health and welfare and the economics of milk production. Comparison of stringently produced cell wall fractions from S. uberis and an isogenic mutant strain lacking SrtA permitted identification of 9 proteins likely to be covalently anchored at the cell surface. Analysis of these sequences implied the presence of two anchoring motifs for S. uberis, the classical LPXTG motif and an additional LPXXXD motif. American Chemical Society 2009-12-29 Article PeerReviewed Egan, Sharon A., Kurian, Dominic, Ward, Philip N., Hunt, Lawrence and Leigh, James A. (2009) Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif. Journal of Proteome Research, 9 (2). pp. 1088-1095. ISSN 1535-3907 Sortase A LPXTG Cell wall proteins Mastitis Streptococcus uberis http://pubs.acs.org/doi/abs/10.1021/pr901025w doi:10.1021/pr901025w doi:10.1021/pr901025w |
| spellingShingle | Sortase A LPXTG Cell wall proteins Mastitis Streptococcus uberis Egan, Sharon A. Kurian, Dominic Ward, Philip N. Hunt, Lawrence Leigh, James A. Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif |
| title | Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif |
| title_full | Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif |
| title_fullStr | Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif |
| title_full_unstemmed | Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif |
| title_short | Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif |
| title_sort | identification of sortase a (srta) substrates in streptococcus uberis: evidence for an additional hexapeptide (lpxxxd) sorting motif |
| topic | Sortase A LPXTG Cell wall proteins Mastitis Streptococcus uberis |
| url | https://eprints.nottingham.ac.uk/37634/ https://eprints.nottingham.ac.uk/37634/ https://eprints.nottingham.ac.uk/37634/ |