Systems analysis of auxin transport in the Arabidopsis root apex
Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the cellular scale is essential to understanding how...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
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American Society of Plant Biologists
2014
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| Online Access: | https://eprints.nottingham.ac.uk/37628/ |
| _version_ | 1848795500662226944 |
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| author | Band, Leah R. Wells, Darren M. Fozard, John A. Ghetiu, Teodor French, Andrew P. Pound, Michael P. Wilson, Michael H. Yu, Lei Li, Wenda Hijazi, Hussein I. Oh, Jaesung Pearce, Simon P. Perez-Amador, Miguel A. Yun, Jeonga Kramer, Eric Alonso, Jose M. Godin, Christophe Vernoux, Teva Hodgman, T. C. Pridmore, Tony P. Swarup, Ranjan King, John R. Bennett, Malcolm J. |
| author_facet | Band, Leah R. Wells, Darren M. Fozard, John A. Ghetiu, Teodor French, Andrew P. Pound, Michael P. Wilson, Michael H. Yu, Lei Li, Wenda Hijazi, Hussein I. Oh, Jaesung Pearce, Simon P. Perez-Amador, Miguel A. Yun, Jeonga Kramer, Eric Alonso, Jose M. Godin, Christophe Vernoux, Teva Hodgman, T. C. Pridmore, Tony P. Swarup, Ranjan King, John R. Bennett, Malcolm J. |
| author_sort | Band, Leah R. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the cellular scale is essential to understanding how these processes are controlled. In this study, we developed an auxin transport model based on actual root cell geometries and carrier subcellular localizations. We tested model predictions using the DII-VENUS auxin sensor in conjunction with state-of-the-art segmentation tools. Our study revealed that auxin efflux carriers alone cannot create the pattern of auxin distribution at the root tip and that AUX1/LAX influx carriers are also required. We observed that AUX1 in lateral root cap (LRC) and elongating epidermal cells greatly enhance auxin’s shootward flux, with this flux being predominantly through the LRC, entering the epidermal cells only as they enter the elongation zone. We conclude that the nonpolar AUX1/LAX influx carriers control which tissues have high auxin levels, whereas the polar PIN carriers control the direction of auxin transport within these tissues. |
| first_indexed | 2025-11-14T19:33:05Z |
| format | Article |
| id | nottingham-37628 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:33:05Z |
| publishDate | 2014 |
| publisher | American Society of Plant Biologists |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-376282020-05-04T16:44:46Z https://eprints.nottingham.ac.uk/37628/ Systems analysis of auxin transport in the Arabidopsis root apex Band, Leah R. Wells, Darren M. Fozard, John A. Ghetiu, Teodor French, Andrew P. Pound, Michael P. Wilson, Michael H. Yu, Lei Li, Wenda Hijazi, Hussein I. Oh, Jaesung Pearce, Simon P. Perez-Amador, Miguel A. Yun, Jeonga Kramer, Eric Alonso, Jose M. Godin, Christophe Vernoux, Teva Hodgman, T. C. Pridmore, Tony P. Swarup, Ranjan King, John R. Bennett, Malcolm J. Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the cellular scale is essential to understanding how these processes are controlled. In this study, we developed an auxin transport model based on actual root cell geometries and carrier subcellular localizations. We tested model predictions using the DII-VENUS auxin sensor in conjunction with state-of-the-art segmentation tools. Our study revealed that auxin efflux carriers alone cannot create the pattern of auxin distribution at the root tip and that AUX1/LAX influx carriers are also required. We observed that AUX1 in lateral root cap (LRC) and elongating epidermal cells greatly enhance auxin’s shootward flux, with this flux being predominantly through the LRC, entering the epidermal cells only as they enter the elongation zone. We conclude that the nonpolar AUX1/LAX influx carriers control which tissues have high auxin levels, whereas the polar PIN carriers control the direction of auxin transport within these tissues. American Society of Plant Biologists 2014-03-14 Article PeerReviewed Band, Leah R., Wells, Darren M., Fozard, John A., Ghetiu, Teodor, French, Andrew P., Pound, Michael P., Wilson, Michael H., Yu, Lei, Li, Wenda, Hijazi, Hussein I., Oh, Jaesung, Pearce, Simon P., Perez-Amador, Miguel A., Yun, Jeonga, Kramer, Eric, Alonso, Jose M., Godin, Christophe, Vernoux, Teva, Hodgman, T. C., Pridmore, Tony P., Swarup, Ranjan, King, John R. and Bennett, Malcolm J. (2014) Systems analysis of auxin transport in the Arabidopsis root apex. Plant Cell, 26 (3). pp. 862-875. ISSN 1040-4651 http://dx.doi.org/10.1105/tpc.113.119495 doi:10.1105/tpc.113.119495 doi:10.1105/tpc.113.119495 |
| spellingShingle | Band, Leah R. Wells, Darren M. Fozard, John A. Ghetiu, Teodor French, Andrew P. Pound, Michael P. Wilson, Michael H. Yu, Lei Li, Wenda Hijazi, Hussein I. Oh, Jaesung Pearce, Simon P. Perez-Amador, Miguel A. Yun, Jeonga Kramer, Eric Alonso, Jose M. Godin, Christophe Vernoux, Teva Hodgman, T. C. Pridmore, Tony P. Swarup, Ranjan King, John R. Bennett, Malcolm J. Systems analysis of auxin transport in the Arabidopsis root apex |
| title | Systems analysis of auxin transport in the Arabidopsis root apex |
| title_full | Systems analysis of auxin transport in the Arabidopsis root apex |
| title_fullStr | Systems analysis of auxin transport in the Arabidopsis root apex |
| title_full_unstemmed | Systems analysis of auxin transport in the Arabidopsis root apex |
| title_short | Systems analysis of auxin transport in the Arabidopsis root apex |
| title_sort | systems analysis of auxin transport in the arabidopsis root apex |
| url | https://eprints.nottingham.ac.uk/37628/ https://eprints.nottingham.ac.uk/37628/ https://eprints.nottingham.ac.uk/37628/ |