Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles
Nanoparticles capable of generating controlled amounts of intracellular reactive oxygen species (ROS), that advance the study of oxidative stress and cellular communication, were synthesized by functionalizing polyacrylamide nanoparticles with zinc(II) porphyrin photosensitisers. Controlled ROS prod...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2015
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| Online Access: | https://eprints.nottingham.ac.uk/37557/ |
| _version_ | 1848795483562049536 |
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| author | Lavado, Andrea S. Chauhan, Veeren M. Alhaj Zen, Amer Giuntini, Francesca Jones, D. Rhodri E. Boyle, Ross W. Beeby, Andrew Chan, Weng C. Aylott, Jonathan W. |
| author_facet | Lavado, Andrea S. Chauhan, Veeren M. Alhaj Zen, Amer Giuntini, Francesca Jones, D. Rhodri E. Boyle, Ross W. Beeby, Andrew Chan, Weng C. Aylott, Jonathan W. |
| author_sort | Lavado, Andrea S. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Nanoparticles capable of generating controlled amounts of intracellular reactive oxygen species (ROS), that advance the study of oxidative stress and cellular communication, were synthesized by functionalizing polyacrylamide nanoparticles with zinc(II) porphyrin photosensitisers. Controlled ROS production was demonstrated in human mesenchymal stem cells (hMSCs) through (1) production of nanoparticles functionalized with varying percentages of Zn(II) porphyrin and (2) modulating the number of doses of excitation light to internalized nanoparticles. hMSCs challenged with nanoparticles functionalized with increasing percentages of Zn(II) porphyrin and high numbers of irradiations of excitation light were found to generate greater amounts of ROS. A novel dye, which is transformed into fluorescent 7-hydroxy-4- trifluoromethyl-coumarin in the presence of hydrogen peroxide, provided an indirect indicator for cumulative ROS production. The mitochondrial membrane potential was monitored to investigate the destructive effect of increased intracellular ROS production. Flow cytometric analysis of nanoparticle treated hMSCs suggested irradiation with excitation light signalled controlled apoptotic cell death, rather than uncontrolled necrotic cell death. Increased intracellular ROS production did not induce phenotypic changes in hMSC subcultures. |
| first_indexed | 2025-11-14T19:32:48Z |
| format | Article |
| id | nottingham-37557 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:32:48Z |
| publishDate | 2015 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-375572020-05-04T17:17:18Z https://eprints.nottingham.ac.uk/37557/ Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles Lavado, Andrea S. Chauhan, Veeren M. Alhaj Zen, Amer Giuntini, Francesca Jones, D. Rhodri E. Boyle, Ross W. Beeby, Andrew Chan, Weng C. Aylott, Jonathan W. Nanoparticles capable of generating controlled amounts of intracellular reactive oxygen species (ROS), that advance the study of oxidative stress and cellular communication, were synthesized by functionalizing polyacrylamide nanoparticles with zinc(II) porphyrin photosensitisers. Controlled ROS production was demonstrated in human mesenchymal stem cells (hMSCs) through (1) production of nanoparticles functionalized with varying percentages of Zn(II) porphyrin and (2) modulating the number of doses of excitation light to internalized nanoparticles. hMSCs challenged with nanoparticles functionalized with increasing percentages of Zn(II) porphyrin and high numbers of irradiations of excitation light were found to generate greater amounts of ROS. A novel dye, which is transformed into fluorescent 7-hydroxy-4- trifluoromethyl-coumarin in the presence of hydrogen peroxide, provided an indirect indicator for cumulative ROS production. The mitochondrial membrane potential was monitored to investigate the destructive effect of increased intracellular ROS production. Flow cytometric analysis of nanoparticle treated hMSCs suggested irradiation with excitation light signalled controlled apoptotic cell death, rather than uncontrolled necrotic cell death. Increased intracellular ROS production did not induce phenotypic changes in hMSC subcultures. Royal Society of Chemistry 2015-09-14 Article PeerReviewed Lavado, Andrea S., Chauhan, Veeren M., Alhaj Zen, Amer, Giuntini, Francesca, Jones, D. Rhodri E., Boyle, Ross W., Beeby, Andrew, Chan, Weng C. and Aylott, Jonathan W. (2015) Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles. Nanoscale, 7 (34). pp. 14525-14531. ISSN 2040-3372 http://pubs.rsc.org/en/Content/ArticleLanding/2015/NR/C5NR00795J#!divAbstract doi:10.1039/c5nr00795j doi:10.1039/c5nr00795j |
| spellingShingle | Lavado, Andrea S. Chauhan, Veeren M. Alhaj Zen, Amer Giuntini, Francesca Jones, D. Rhodri E. Boyle, Ross W. Beeby, Andrew Chan, Weng C. Aylott, Jonathan W. Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles |
| title | Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles |
| title_full | Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles |
| title_fullStr | Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles |
| title_full_unstemmed | Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles |
| title_short | Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles |
| title_sort | controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles |
| url | https://eprints.nottingham.ac.uk/37557/ https://eprints.nottingham.ac.uk/37557/ https://eprints.nottingham.ac.uk/37557/ |