A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain

Rice is a major source of calories and mineral nutrients for over half the world’s human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of...

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Main Authors: Huang, Xin-Yuan, Deng, Fenglin, Yamaji, Naoki, Pinson, Shannon R.M.., Fujii-Kashino, Miho, Danku, John, Douglas, Alex, Guerinot, Mary Lou, Salt, David E., Ma, Jian Feng
Format: Article
Published: Nature Publishing Group 2016
Online Access:https://eprints.nottingham.ac.uk/37471/
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author Huang, Xin-Yuan
Deng, Fenglin
Yamaji, Naoki
Pinson, Shannon R.M..
Fujii-Kashino, Miho
Danku, John
Douglas, Alex
Guerinot, Mary Lou
Salt, David E.
Ma, Jian Feng
author_facet Huang, Xin-Yuan
Deng, Fenglin
Yamaji, Naoki
Pinson, Shannon R.M..
Fujii-Kashino, Miho
Danku, John
Douglas, Alex
Guerinot, Mary Lou
Salt, David E.
Ma, Jian Feng
author_sort Huang, Xin-Yuan
building Nottingham Research Data Repository
collection Online Access
description Rice is a major source of calories and mineral nutrients for over half the world’s human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs.
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spelling nottingham-374712020-05-04T18:02:47Z https://eprints.nottingham.ac.uk/37471/ A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain Huang, Xin-Yuan Deng, Fenglin Yamaji, Naoki Pinson, Shannon R.M.. Fujii-Kashino, Miho Danku, John Douglas, Alex Guerinot, Mary Lou Salt, David E. Ma, Jian Feng Rice is a major source of calories and mineral nutrients for over half the world’s human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs. Nature Publishing Group 2016-07-08 Article PeerReviewed Huang, Xin-Yuan, Deng, Fenglin, Yamaji, Naoki, Pinson, Shannon R.M.., Fujii-Kashino, Miho, Danku, John, Douglas, Alex, Guerinot, Mary Lou, Salt, David E. and Ma, Jian Feng (2016) A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain. Nature Communications, 7 (12138). pp. 1-13. ISSN 2041-1723 http://www.nature.com/articles/ncomms12138 doi:10.1038/ncomms12138 doi:10.1038/ncomms12138
spellingShingle Huang, Xin-Yuan
Deng, Fenglin
Yamaji, Naoki
Pinson, Shannon R.M..
Fujii-Kashino, Miho
Danku, John
Douglas, Alex
Guerinot, Mary Lou
Salt, David E.
Ma, Jian Feng
A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain
title A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain
title_full A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain
title_fullStr A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain
title_full_unstemmed A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain
title_short A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain
title_sort heavy metal p-type atpase oshma4 prevents copper accumulation in rice grain
url https://eprints.nottingham.ac.uk/37471/
https://eprints.nottingham.ac.uk/37471/
https://eprints.nottingham.ac.uk/37471/