Sequence and partial functional analysis of canine Bcl-2 family proteins
Dogs present with spontaneous neoplasms biologically similar to human cancers. Apoptotic pathways are deregulated during cancer genesis and progression and are important for therapy. We have assessed the degree of conservation of a set of canine Bcl-2 family members with the human and murine ortholo...
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| Format: | Article |
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/37341/ |
| _version_ | 1848795440116400128 |
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| author | de Brot, Simone Schade, Benjamin Croci, Martina Dettwiler, Martina Guscetti, Franco |
| author_facet | de Brot, Simone Schade, Benjamin Croci, Martina Dettwiler, Martina Guscetti, Franco |
| author_sort | de Brot, Simone |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Dogs present with spontaneous neoplasms biologically similar to human cancers. Apoptotic pathways are deregulated during cancer genesis and progression and are important for therapy. We have assessed the degree of conservation of a set of canine Bcl-2 family members with the human and murine orthologs. To this end, seven complete canine open reading frames were cloned in this family, four of which are novel for the dog, their sequences were analyzed, and their functional interactions were studied in yeasts. We found a high degree of overall and domain sequence homology between canine and human proteins. It was slightly higher than between murine and human proteins. Functional interactions between canine pro-apoptotic Bax and Bak and anti-apoptotic Bcl-xL, Bcl-w, and Mcl-1 were recapitulated in yeasts. Our data provide support for the notion that systems based on canine-derived proteins might faithfully reproduce Bcl-2 family member interactions known from other species and establish the yeast as a useful tool for functional studies with canine proteins. |
| first_indexed | 2025-11-14T19:32:07Z |
| format | Article |
| id | nottingham-37341 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:32:07Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-373412020-05-04T17:35:09Z https://eprints.nottingham.ac.uk/37341/ Sequence and partial functional analysis of canine Bcl-2 family proteins de Brot, Simone Schade, Benjamin Croci, Martina Dettwiler, Martina Guscetti, Franco Dogs present with spontaneous neoplasms biologically similar to human cancers. Apoptotic pathways are deregulated during cancer genesis and progression and are important for therapy. We have assessed the degree of conservation of a set of canine Bcl-2 family members with the human and murine orthologs. To this end, seven complete canine open reading frames were cloned in this family, four of which are novel for the dog, their sequences were analyzed, and their functional interactions were studied in yeasts. We found a high degree of overall and domain sequence homology between canine and human proteins. It was slightly higher than between murine and human proteins. Functional interactions between canine pro-apoptotic Bax and Bak and anti-apoptotic Bcl-xL, Bcl-w, and Mcl-1 were recapitulated in yeasts. Our data provide support for the notion that systems based on canine-derived proteins might faithfully reproduce Bcl-2 family member interactions known from other species and establish the yeast as a useful tool for functional studies with canine proteins. Elsevier 2016-02-28 Article PeerReviewed de Brot, Simone, Schade, Benjamin, Croci, Martina, Dettwiler, Martina and Guscetti, Franco (2016) Sequence and partial functional analysis of canine Bcl-2 family proteins. Research in Veterinary Science, 104 . pp. 126-135. ISSN 1532-2661 Bcl-2 family; Canine; Cloning; Comparative sequence analysis; Functional yeast study http://www.sciencedirect.com/science/article/pii/S0034528815301028 doi:10.1016/j.rvsc.2015.12.001 doi:10.1016/j.rvsc.2015.12.001 |
| spellingShingle | Bcl-2 family; Canine; Cloning; Comparative sequence analysis; Functional yeast study de Brot, Simone Schade, Benjamin Croci, Martina Dettwiler, Martina Guscetti, Franco Sequence and partial functional analysis of canine Bcl-2 family proteins |
| title | Sequence and partial functional analysis of canine Bcl-2 family proteins |
| title_full | Sequence and partial functional analysis of canine Bcl-2 family proteins |
| title_fullStr | Sequence and partial functional analysis of canine Bcl-2 family proteins |
| title_full_unstemmed | Sequence and partial functional analysis of canine Bcl-2 family proteins |
| title_short | Sequence and partial functional analysis of canine Bcl-2 family proteins |
| title_sort | sequence and partial functional analysis of canine bcl-2 family proteins |
| topic | Bcl-2 family; Canine; Cloning; Comparative sequence analysis; Functional yeast study |
| url | https://eprints.nottingham.ac.uk/37341/ https://eprints.nottingham.ac.uk/37341/ https://eprints.nottingham.ac.uk/37341/ |