The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment

In this study, we have produced nanotextured poly(lactic-co-glycolic acid) (PLGA) films by using polystyrene (PS) particles as a template to make a polydimethylsiloxane mould against which PLGA is solvent cast. Biocompatible, biodegradable and nanotextured PLGA films were prepared with PS particles...

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Main Authors: Yaşayan, Gökçen, Xue, Xuan, Collier, Pamela, Clarke, Philip, Alexander, Morgan R., Marlow, Maria
Format: Article
Published: IOP Publishing 2016
Subjects:
Online Access:https://eprints.nottingham.ac.uk/37328/
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author Yaşayan, Gökçen
Xue, Xuan
Collier, Pamela
Clarke, Philip
Alexander, Morgan R.
Marlow, Maria
author_facet Yaşayan, Gökçen
Xue, Xuan
Collier, Pamela
Clarke, Philip
Alexander, Morgan R.
Marlow, Maria
author_sort Yaşayan, Gökçen
building Nottingham Research Data Repository
collection Online Access
description In this study, we have produced nanotextured poly(lactic-co-glycolic acid) (PLGA) films by using polystyrene (PS) particles as a template to make a polydimethylsiloxane mould against which PLGA is solvent cast. Biocompatible, biodegradable and nanotextured PLGA films were prepared with PS particles of diameter of 57, 99, 210, and 280 nm that produced domes of the same dimension in the PLGA surface. The effect of the particulate monolayer templating method was investigated to enable preparation of the films with uniformly ordered surface nanodomes. Cell attachment of a human ovarian cancer cell line (OVCAR3) alone and co-cultured with mesenchymal stem cells (MSCs) was evaluated on flat and topographically nano-patterned surfaces. Cell numbers were observed to increase on the nanotextured surfaces compared to non-textured surfaces both with OVCAR3 cultures and OVCAR3-MSC co-cultures at 24 and 48 h time points.
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publishDate 2016
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spelling nottingham-373282020-05-04T17:51:40Z https://eprints.nottingham.ac.uk/37328/ The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment Yaşayan, Gökçen Xue, Xuan Collier, Pamela Clarke, Philip Alexander, Morgan R. Marlow, Maria In this study, we have produced nanotextured poly(lactic-co-glycolic acid) (PLGA) films by using polystyrene (PS) particles as a template to make a polydimethylsiloxane mould against which PLGA is solvent cast. Biocompatible, biodegradable and nanotextured PLGA films were prepared with PS particles of diameter of 57, 99, 210, and 280 nm that produced domes of the same dimension in the PLGA surface. The effect of the particulate monolayer templating method was investigated to enable preparation of the films with uniformly ordered surface nanodomes. Cell attachment of a human ovarian cancer cell line (OVCAR3) alone and co-cultured with mesenchymal stem cells (MSCs) was evaluated on flat and topographically nano-patterned surfaces. Cell numbers were observed to increase on the nanotextured surfaces compared to non-textured surfaces both with OVCAR3 cultures and OVCAR3-MSC co-cultures at 24 and 48 h time points. IOP Publishing 2016-05-17 Article PeerReviewed Yaşayan, Gökçen, Xue, Xuan, Collier, Pamela, Clarke, Philip, Alexander, Morgan R. and Marlow, Maria (2016) The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment. Nanotechnology, 27 (25). p. 255102. ISSN 1361-6528 nanotexturing films poly(lactic-co-glycolic acid) human ovarian cancer cell http://iopscience.iop.org/article/10.1088/0957-4484/27/25/255102/meta doi:10.1088/0957-4484/27/25/255102 doi:10.1088/0957-4484/27/25/255102
spellingShingle nanotexturing
films
poly(lactic-co-glycolic acid)
human ovarian cancer cell
Yaşayan, Gökçen
Xue, Xuan
Collier, Pamela
Clarke, Philip
Alexander, Morgan R.
Marlow, Maria
The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment
title The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment
title_full The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment
title_fullStr The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment
title_full_unstemmed The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment
title_short The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment
title_sort influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment
topic nanotexturing
films
poly(lactic-co-glycolic acid)
human ovarian cancer cell
url https://eprints.nottingham.ac.uk/37328/
https://eprints.nottingham.ac.uk/37328/
https://eprints.nottingham.ac.uk/37328/