Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap
We present a new technique for the study of model membranes on the length-scale of a single nanosized liposome. Silver decahedral nanoparticles have been encapsulated by a model unilamellar lipid bilayer creating nano-sized lipid vesicles. The metal core has two roles (i) increasing the polarizabili...
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| Format: | Article |
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Royal Society of Chemistry
2016
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| Online Access: | https://eprints.nottingham.ac.uk/37282/ |
| _version_ | 1848795426149367808 |
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| author | Wright, Amanda J. Richens, Joanna L. Bramble, J.P. Cathcart, N. Kitaev, V. O'Shea, Paul Hudson, A.J. |
| author_facet | Wright, Amanda J. Richens, Joanna L. Bramble, J.P. Cathcart, N. Kitaev, V. O'Shea, Paul Hudson, A.J. |
| author_sort | Wright, Amanda J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | We present a new technique for the study of model membranes on the length-scale of a single nanosized liposome. Silver decahedral nanoparticles have been encapsulated by a model unilamellar lipid bilayer creating nano-sized lipid vesicles. The metal core has two roles (i) increasing the polarizability of vesicles, enabling a single vesicle to be isolated and confined in an optical trap, and (ii) enhancing Raman scattering from the bilayer, via the high surface-plasmon field at the sharp vertices of the decahedral particles. Combined this has allowed us to measure a Raman fingerprint from a single vesicle of 50 nmdiameter, containing just ∼104 lipid molecules in a bilayer membrane over a surface area of <0.01 µm2, equivalent to a volume of approximately 1 zepto-litre. Raman scattering is a weak and inefficient process and previous studies have required either a substantially larger bilayer area in order to obtain a detectable signal, or the tagging of lipid molecules with a chromophore to provide an indirect probe of the bilayer. Our approach is fully label-free and bio-compatible and, in the future, it will enable much more localized studies of the heterogeneous structure of lipid bilayers and of membrane-bound components than is currently possible. |
| first_indexed | 2025-11-14T19:31:54Z |
| format | Article |
| id | nottingham-37282 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:31:54Z |
| publishDate | 2016 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-372822024-08-15T15:20:26Z https://eprints.nottingham.ac.uk/37282/ Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap Wright, Amanda J. Richens, Joanna L. Bramble, J.P. Cathcart, N. Kitaev, V. O'Shea, Paul Hudson, A.J. We present a new technique for the study of model membranes on the length-scale of a single nanosized liposome. Silver decahedral nanoparticles have been encapsulated by a model unilamellar lipid bilayer creating nano-sized lipid vesicles. The metal core has two roles (i) increasing the polarizability of vesicles, enabling a single vesicle to be isolated and confined in an optical trap, and (ii) enhancing Raman scattering from the bilayer, via the high surface-plasmon field at the sharp vertices of the decahedral particles. Combined this has allowed us to measure a Raman fingerprint from a single vesicle of 50 nmdiameter, containing just ∼104 lipid molecules in a bilayer membrane over a surface area of <0.01 µm2, equivalent to a volume of approximately 1 zepto-litre. Raman scattering is a weak and inefficient process and previous studies have required either a substantially larger bilayer area in order to obtain a detectable signal, or the tagging of lipid molecules with a chromophore to provide an indirect probe of the bilayer. Our approach is fully label-free and bio-compatible and, in the future, it will enable much more localized studies of the heterogeneous structure of lipid bilayers and of membrane-bound components than is currently possible. Royal Society of Chemistry 2016-09-02 Article PeerReviewed Wright, Amanda J., Richens, Joanna L., Bramble, J.P., Cathcart, N., Kitaev, V., O'Shea, Paul and Hudson, A.J. (2016) Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap. Nanoscale, 8 (36). pp. 16395-16404. ISSN 2040-3364 http://pubs.rsc.org/en/Content/ArticleLanding/2016/NR/C6NR05616D#!divAbstract doi:10.1039/C6NR05616D doi:10.1039/C6NR05616D |
| spellingShingle | Wright, Amanda J. Richens, Joanna L. Bramble, J.P. Cathcart, N. Kitaev, V. O'Shea, Paul Hudson, A.J. Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap |
| title | Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap |
| title_full | Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap |
| title_fullStr | Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap |
| title_full_unstemmed | Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap |
| title_short | Surface-enhanced Raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap |
| title_sort | surface-enhanced raman scattering measurement from a lipid bilayer encapsulating a single decahedral nanoparticle mediated by an optical trap |
| url | https://eprints.nottingham.ac.uk/37282/ https://eprints.nottingham.ac.uk/37282/ https://eprints.nottingham.ac.uk/37282/ |