Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes
Oocytes treated with the protein synthesis inhibitor cycloheximide (CHX) arrest at the germinal vesicle (GV) stage and undergo accelerated GV breakdown (GVBD) after CHX is removed. However, little is known about the underlying mechanism of accelerated meiotic maturation. Here, we investigated this m...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Society for the Study of Reproduction
2015
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/37068/ |
| _version_ | 1848795386005684224 |
|---|---|
| author | German, Sergio D. Lee, Joon-Hee Campbell, Keith H. Sweetman, Dylan Alberio, Ramiro |
| author_facet | German, Sergio D. Lee, Joon-Hee Campbell, Keith H. Sweetman, Dylan Alberio, Ramiro |
| author_sort | German, Sergio D. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Oocytes treated with the protein synthesis inhibitor cycloheximide (CHX) arrest at the germinal vesicle (GV) stage and undergo accelerated GV breakdown (GVBD) after CHX is removed. However, little is known about the underlying mechanism of accelerated meiotic maturation. Here, we investigated this mechanism and found that oocytes released from CHX arrest have higher amounts of cyclin B1 (CCNB1) and phosphorylated mitogen-activated protein kinase (pMAPK) proteins. Increased levels of these factors were not associated with mRNA polyadenylation or increased transcription rates of CCNB1 and MOS (Moloney murine sarcoma viral oncogene homolog) during CHX arrest. We found that treatment of CHX-arrested oocytes with the actin filament-stabilizing agent Jasplakinolide (Jasp) delayed GVBD following release from CHX arrest and that this was correlated with reduced maturation-promoting factor (MPF) activity. These results suggest that CCNB1 mRNAs released from actin filaments during CHX arrest increase CCNB1 transcripts available for translation after release from CHX arrest, leading to the precocious activation of MPF and accelerated meiotic progression. |
| first_indexed | 2025-11-14T19:31:15Z |
| format | Article |
| id | nottingham-37068 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:31:15Z |
| publishDate | 2015 |
| publisher | Society for the Study of Reproduction |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-370682020-05-04T17:04:29Z https://eprints.nottingham.ac.uk/37068/ Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes German, Sergio D. Lee, Joon-Hee Campbell, Keith H. Sweetman, Dylan Alberio, Ramiro Oocytes treated with the protein synthesis inhibitor cycloheximide (CHX) arrest at the germinal vesicle (GV) stage and undergo accelerated GV breakdown (GVBD) after CHX is removed. However, little is known about the underlying mechanism of accelerated meiotic maturation. Here, we investigated this mechanism and found that oocytes released from CHX arrest have higher amounts of cyclin B1 (CCNB1) and phosphorylated mitogen-activated protein kinase (pMAPK) proteins. Increased levels of these factors were not associated with mRNA polyadenylation or increased transcription rates of CCNB1 and MOS (Moloney murine sarcoma viral oncogene homolog) during CHX arrest. We found that treatment of CHX-arrested oocytes with the actin filament-stabilizing agent Jasplakinolide (Jasp) delayed GVBD following release from CHX arrest and that this was correlated with reduced maturation-promoting factor (MPF) activity. These results suggest that CCNB1 mRNAs released from actin filaments during CHX arrest increase CCNB1 transcripts available for translation after release from CHX arrest, leading to the precocious activation of MPF and accelerated meiotic progression. Society for the Study of Reproduction 2015-03-18 Article PeerReviewed German, Sergio D., Lee, Joon-Hee, Campbell, Keith H., Sweetman, Dylan and Alberio, Ramiro (2015) Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes. Biology of Reproduction, 92 (4). 103-1-103-9. ISSN 1529-7268 actin depolymerization cyclin B1 cycloheximide cytoskeleton GVBD IVM meiotic arrest meiotic maturation oocyte maturation polyadenylation protein kinases RNA granules http://www.biolreprod.org/content/92/4/103 doi:10.1095/biolreprod.114.122341 doi:10.1095/biolreprod.114.122341 |
| spellingShingle | actin depolymerization cyclin B1 cycloheximide cytoskeleton GVBD IVM meiotic arrest meiotic maturation oocyte maturation polyadenylation protein kinases RNA granules German, Sergio D. Lee, Joon-Hee Campbell, Keith H. Sweetman, Dylan Alberio, Ramiro Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes |
| title | Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes |
| title_full | Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes |
| title_fullStr | Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes |
| title_full_unstemmed | Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes |
| title_short | Actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes |
| title_sort | actin depolymerization is associated with meiotic acceleration in cycloheximide-treated ovine oocytes |
| topic | actin depolymerization cyclin B1 cycloheximide cytoskeleton GVBD IVM meiotic arrest meiotic maturation oocyte maturation polyadenylation protein kinases RNA granules |
| url | https://eprints.nottingham.ac.uk/37068/ https://eprints.nottingham.ac.uk/37068/ https://eprints.nottingham.ac.uk/37068/ |