The application of protein microarray assays in psychoneuroimmunology
Protein microarrays are miniaturized multiplex assays that exhibit many advantages over the commonly used enzyme-linked immunosorbent assay (ELISA). This article aims to introduce protein microarrays to readers of Brain, Behavior, and Immunity and demonstrate its utility and validity for use in psyc...
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| Format: | Article |
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/37067/ |
| _version_ | 1848795385732005888 |
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| author | Ayling, K. Bowden, T. Tighe, Patrick J. Todd, Ian Dilnot, Elizabeth M. Hegm, O.H. Fairclough, Lucy C. Vedhara, Kavita |
| author_facet | Ayling, K. Bowden, T. Tighe, Patrick J. Todd, Ian Dilnot, Elizabeth M. Hegm, O.H. Fairclough, Lucy C. Vedhara, Kavita |
| author_sort | Ayling, K. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Protein microarrays are miniaturized multiplex assays that exhibit many advantages over the commonly used enzyme-linked immunosorbent assay (ELISA). This article aims to introduce protein microarrays to readers of Brain, Behavior, and Immunity and demonstrate its utility and validity for use in psychoneuroimmunological research. As part of an ongoing investigation of psychological and behavioral influences on influenza vaccination responses, we optimized a novel protein microarray to quantify influenza-specific antibody levels in human sera. Reproducibility was assessed by calculating intra- and inter-assay coefficients of variance on serially diluted human IgG concentrations. A random selection of samples was analyzed by microarray and ELISA to establish validity of the assay. For IgG concentrations, intra-assay and inter-assay precision profiles demonstrated a mean coefficient of variance of 6.7% and 11.5% respectively. Significant correlations were observed between microarray and ELISA for all antigens, demonstrating the microarray is a valid alternative to ELISA. Protein microarrays are a highly robust, novel assay method that could be of significant benefit for researchers working in psychoneuroimmunology. They offer high throughput, fewer resources per analyte and can examine concurrent neuro-immune-endocrine mechanisms. |
| first_indexed | 2025-11-14T19:31:15Z |
| format | Article |
| id | nottingham-37067 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:31:15Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-370672020-05-04T18:28:46Z https://eprints.nottingham.ac.uk/37067/ The application of protein microarray assays in psychoneuroimmunology Ayling, K. Bowden, T. Tighe, Patrick J. Todd, Ian Dilnot, Elizabeth M. Hegm, O.H. Fairclough, Lucy C. Vedhara, Kavita Protein microarrays are miniaturized multiplex assays that exhibit many advantages over the commonly used enzyme-linked immunosorbent assay (ELISA). This article aims to introduce protein microarrays to readers of Brain, Behavior, and Immunity and demonstrate its utility and validity for use in psychoneuroimmunological research. As part of an ongoing investigation of psychological and behavioral influences on influenza vaccination responses, we optimized a novel protein microarray to quantify influenza-specific antibody levels in human sera. Reproducibility was assessed by calculating intra- and inter-assay coefficients of variance on serially diluted human IgG concentrations. A random selection of samples was analyzed by microarray and ELISA to establish validity of the assay. For IgG concentrations, intra-assay and inter-assay precision profiles demonstrated a mean coefficient of variance of 6.7% and 11.5% respectively. Significant correlations were observed between microarray and ELISA for all antigens, demonstrating the microarray is a valid alternative to ELISA. Protein microarrays are a highly robust, novel assay method that could be of significant benefit for researchers working in psychoneuroimmunology. They offer high throughput, fewer resources per analyte and can examine concurrent neuro-immune-endocrine mechanisms. Elsevier 2017-01-31 Article PeerReviewed Ayling, K., Bowden, T., Tighe, Patrick J., Todd, Ian, Dilnot, Elizabeth M., Hegm, O.H., Fairclough, Lucy C. and Vedhara, Kavita (2017) The application of protein microarray assays in psychoneuroimmunology. Brain, Behavior and Immunity, 59 . pp. 62-66. ISSN 1090-2139 Microarray; ELISA; Psychoneuroimmunology; Sera; Multiplex; Assay http://www.sciencedirect.com/science/article/pii/S0889159116304226 doi:10.1016/j.bbi.2016.09.013 doi:10.1016/j.bbi.2016.09.013 |
| spellingShingle | Microarray; ELISA; Psychoneuroimmunology; Sera; Multiplex; Assay Ayling, K. Bowden, T. Tighe, Patrick J. Todd, Ian Dilnot, Elizabeth M. Hegm, O.H. Fairclough, Lucy C. Vedhara, Kavita The application of protein microarray assays in psychoneuroimmunology |
| title | The application of protein microarray assays in psychoneuroimmunology |
| title_full | The application of protein microarray assays in psychoneuroimmunology |
| title_fullStr | The application of protein microarray assays in psychoneuroimmunology |
| title_full_unstemmed | The application of protein microarray assays in psychoneuroimmunology |
| title_short | The application of protein microarray assays in psychoneuroimmunology |
| title_sort | application of protein microarray assays in psychoneuroimmunology |
| topic | Microarray; ELISA; Psychoneuroimmunology; Sera; Multiplex; Assay |
| url | https://eprints.nottingham.ac.uk/37067/ https://eprints.nottingham.ac.uk/37067/ https://eprints.nottingham.ac.uk/37067/ |