An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms
A key aim in microbiology is to determine the genetic and phenotypic bases of bacterial virulence, persistence and antimicrobial resistance in chronic biofilm infections. This requires tractable, high-throughput models that reflect the physical and chemical environment encountered in specific infect...
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| Format: | Article |
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Society for General Microbiology
2016
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| Online Access: | https://eprints.nottingham.ac.uk/35952/ |
| _version_ | 1848795197092134912 |
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| author | Harrison, Freya Diggle, Stephen P. |
| author_facet | Harrison, Freya Diggle, Stephen P. |
| author_sort | Harrison, Freya |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | A key aim in microbiology is to determine the genetic and phenotypic bases of bacterial virulence, persistence and antimicrobial resistance in chronic biofilm infections. This requires tractable, high-throughput models that reflect the physical and chemical environment encountered in specific infection contexts. Such models will increase the predictive power of microbiological experiments and provide platforms for enhanced testing of novel antibacterial or antivirulence therapies. We present an optimised ex vivo model of cystic fibrosis lung infection: ex vivo culture of pig bronchiolar tissue in artificial cystic fibrosis mucus. We focus on the formation of biofilms by Pseudomonas aeruginosa. We show highly repeatable and specific formation of biofilms that resemble clinical biofilms by a commonly-studied lab strain and ten cystic fibrosis isolates of this key opportunistic pathogen. |
| first_indexed | 2025-11-14T19:28:15Z |
| format | Article |
| id | nottingham-35952 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:28:15Z |
| publishDate | 2016 |
| publisher | Society for General Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-359522020-05-04T18:08:19Z https://eprints.nottingham.ac.uk/35952/ An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms Harrison, Freya Diggle, Stephen P. A key aim in microbiology is to determine the genetic and phenotypic bases of bacterial virulence, persistence and antimicrobial resistance in chronic biofilm infections. This requires tractable, high-throughput models that reflect the physical and chemical environment encountered in specific infection contexts. Such models will increase the predictive power of microbiological experiments and provide platforms for enhanced testing of novel antibacterial or antivirulence therapies. We present an optimised ex vivo model of cystic fibrosis lung infection: ex vivo culture of pig bronchiolar tissue in artificial cystic fibrosis mucus. We focus on the formation of biofilms by Pseudomonas aeruginosa. We show highly repeatable and specific formation of biofilms that resemble clinical biofilms by a commonly-studied lab strain and ten cystic fibrosis isolates of this key opportunistic pathogen. Society for General Microbiology 2016-10-01 Article PeerReviewed Harrison, Freya and Diggle, Stephen P. (2016) An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms. Microbiology, 162 . pp. 1755-1760. ISSN 1465-2080 Biofilm 3Rs Chronic infection Cystic fibrosis Respiratory infection Pseudomonas aeruginosa http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000352 doi:10.1099/mic.0.000352 doi:10.1099/mic.0.000352 |
| spellingShingle | Biofilm 3Rs Chronic infection Cystic fibrosis Respiratory infection Pseudomonas aeruginosa Harrison, Freya Diggle, Stephen P. An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms |
| title | An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms |
| title_full | An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms |
| title_fullStr | An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms |
| title_full_unstemmed | An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms |
| title_short | An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms |
| title_sort | ex vivo lung model to study bronchioles infected with pseudomonas aeruginosa biofilms |
| topic | Biofilm 3Rs Chronic infection Cystic fibrosis Respiratory infection Pseudomonas aeruginosa |
| url | https://eprints.nottingham.ac.uk/35952/ https://eprints.nottingham.ac.uk/35952/ https://eprints.nottingham.ac.uk/35952/ |