A genetic assay for gene essentiality in Clostridium

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A genetic assay for gene essentiality in Clostridium

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a sta...

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Bibliographic Details
Main Authors:	Walker, David J.F., Heap, John T., Winzer, Klaus, Minton, Nigel P.
Format:	Article
Published:	Elsevier 2016
Subjects:	Clostridium difficile; ClosTron; merodiploid; gene essentiality; therapeutic target
Online Access:	https://eprints.nottingham.ac.uk/35788/

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